Morphological and molecular description of Longidorus behshahrensis n. sp. (Nematoda: Longidoridae) from natural forests of Abbas Abad, north of Iran

  • Shahrbanou Bakhshi Amrei
  • Vlada Peneva
  • Farshad Rakhshandehroo
  • Majid PedramEmail author


Longidorus behshahrensis n. sp. is described and illustrated using morphological and molecular data. It was recovered from the rhizosphere of a wild cherry tree in Mazandaran province, northeastern Iran. The new species is characterized by having 6.8–8.4 mm long females, lip region separated from the rest of the body by a shallow depression, amphidial fovea pocket shaped not bilobed, guiding ring at 25–31 μm from the anterior end, 81–91 and 51–63 μm long odontostyle and odontophore, respectively, 109–130 μm long pharyngeal bulb, didelphic-amphidelphic reproductive system with long tubular bipartite uteri with sperm, vulva at 44–54%, tail bluntly conical, dorsally convex, ventrally concave with widely rounded terminus, hyaline region with discernible radial lines; males common with 50–60 μm long spicules and 9–11 ventromedian single supplements. Only the two latter juvenile developmental stages were recovered for the new species. Codes for identifying the new species are A3-B1-C23-D2-E1-F34-G23-H2-I2. Morphologically, the new species comes close to eight known species of the genus namely L. artemisiae, L. dunensis, L. elongatus, L. euonymus, L. goodeyi, L. iranicus, L. protae and L. tabrizicus. The morphological differences of the new species and the aforementioned species are discussed. In molecular phylogenetic analyses using LSU rDNA D2-D3 sequences, the position of the new species among closely related species was not resolved due to polytomy. In ITS1 phylogeny, the new species formed a clade with L. distinctus.


Bayesian ITS1 LSU rDNA D2-D3 Mazandaran province Phylogeny Taxonomy 



We acknowledge the Science and Research Branch of Islamic Azad University (Iran) and Tarbiat Modares University (Iran) for their financial support. The study was partly supported by BAS, ANIDIV3 project.

Compliance with ethical standards

Conflict of interest

All the authors certify that they do not have any conflict of interest, the presented data are original and the described species is not published, and is not under evaluation for publication elsewhere, all prevailing local, national and international regulations and conventions, and normal scientific ethical practices, have been respected. They also certify that the final version of this manuscript has been reviewed by all authors.

Research involving human participants and/or animals

No specific permits were required for this study. The soil samples analyzed in this study were obtained in public areas, forests, and grasslands, that are not under protection.

Informed consent

The authors certify that they have accepted the principles of ethical standards and that guidelines of professional conduct have been followed. The funders had no role in designing of this study, collecting and analyzing of the data, preparing the manuscript and are not involved in evaluating or publishing process of this research.

Supplementary material

10658_2019_1884_MOESM1_ESM.jpg (550 kb)
ESM 1 The LSU Bayesian tree inferred using maximal number of available data. This tree is used to infer a pruned smaller LSU tree. (JPG 550 kb)


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  • Shahrbanou Bakhshi Amrei
    • 1
  • Vlada Peneva
    • 2
  • Farshad Rakhshandehroo
    • 1
  • Majid Pedram
    • 3
    Email author
  1. 1.Department of Plant Protection, College of Agricultural Sciences and Food Industries, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Institute of Biodiversity and Ecosystem ResearchBulgarian Academy of SciencesSofiaBulgaria
  3. 3.Department of Plant Pathology, Faculty of AgricultureTarbiat Modares UniversityTehranIran

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