The distribution and nucleotide sequences of chrysanthemum stunt viroid in dahlia fields in Japan and the effect of its infection on the growth of dahlias

  • Shunsuke AsanoEmail author
  • Kandai Yoshida
  • Terufumi Naka
  • Yoshihiko Hirayama
  • Yosuke Matsushita


Chrysanthemum stunt viroid (CSVd) is known to infect a wide range of Dahlia spp; however, to the best of our knowledge, no detailed study has been conducted on the distribution of CSVd variants, the infection efficacy of sap inoculations, and the symptoms caused by infection of CSVd in dahlias. We have detected CSVd in 94 of 214 dahlia samples, from across eight different Japanese Prefectures, using RT-PCR.

Six sequence variants of CSVd were detected from 32 CSVd-infected dahlia samples including 3 variants which have not been previously reported and three previously isolated variants from dahlias or chrysanthemums. Five CSVd variants infected and replicated in both dahlia and chrysanthemum plants by sap and RNA inoculation three months post inoculation. The sequence mutations among those variants did not significantly affect the infection efficacy.

Infection of CSVd significantly reduced fresh plant weight, plant height, size of compound leaves, flower diameter, and fresh flower weight of dahlias in two cultivars, though it did not affect the number of nodes. In addition, it altered the color of ray florets and increased the rate at which flowers exposed their disks and that of disk florets. The altered color of ray florets in the ‘Magokoro’ cultivar were darker pink and those of the ‘Portlight pair beauty’ cultivar were darker orange than the uninfected samples.

Our results show that CSVd infects a wide range of dahlias in the field and is an important pathogen to manage for their cultivation as it negatively affects their productivity.


CSVd Stunting Sequence variant 



infections affect growth of dahlias



We thank Mr. Yoshida, Ms. Funato, Ms. Hayashi, Ms. Onji and Ms. Yamanaka for technical assistance.

Compliance with ethical standards

The authors declare that the work is in compliance with ethical standards.

Disclosure of potential conflicts of interest

The authors declare that they have no disclosure of potential conflicts of interest.

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

The authors declare that the manuscript does not contain research involving Human Participants and/or Animals.


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  1. 1.Nara Prefecture Agricultural Research and Development CenterNaraJapan
  2. 2.Institute of Vegetable and Floriculture ScienceNAROIbarakiJapan

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