Cinnamon powder: an in vitro and in vivo evaluation of antifungal and plant growth promoting activity
In this study, the effects of organic powder of Cinnamomum zeylanicum on the development of Botrytis cinerea and its influence on tomato plants were evaluated. The cinnamon bark powder and its water suspensions and filtrates were used at 0.5 and 1% rates. After 6 days of the start of an in vitro experiment the mycelium growth was inhibited by both 0.5 and 1% cinnamon water filtrates - to a greater degree in the case of the higher concentration, by 54.4 and 81.4%, respectively. Spraying with cinnamon water filtrates positively influenced the growth of plants both in the greenhouse and the field. Antifungal activity of cinnamon was proved in the greenhouse tests - the disease symptoms of grey mould on infected tomato plants decreased. The fresh weight of non-inoculated tomato plants treated with cinnamon filtrates was significantly higher than control plants (17.17 g compared to 12.83 g) showing a stimulating effect of cinnamon filtrates. In the case of inoculated plants due to treatment, their weight increased from 7.83 to 10.50 g. In the field experiment, tomato plants sprayed six times with cinnamon were better developed than the control plants. The most significant effect was observed for Hamlet variety - the mean number of leaves was higher by 27.3% and the mean number of branches by 19.7% compared to the untreated control plants. Thus it was proved that cinnamon powder has potential to inhibit B. cinerea growth and also has a stimulating effect for tomato plants.
KeywordsBotrytis cinerea Basic substance Mycelium growth inhibition Grey mould symptoms inhibition Tomato vegetative development
There is a worldwide trend to explore new alternatives to control plant diseases, giving priority to methods that reduce disease symptoms and avoid negative side effects on human health resulting from excessive application of synthetic fungicides. The use of food substances, which are also classified as basic substances, demonstrating a protective action in growing crops, is an alternative method for organic farmers or sustainable agriculture farms.
The possibility of limiting the development of pathogenic fungi by applying agents, which are natural and alternative to pesticides, is a popular research direction. But also the use of plants or plant products as fungicides has been of a great importance for some time and needs more attention (Zaker 2016). New pesticides, including natural product-based pesticides are being discovered and developed to replace the compounds lost due to the new registration requirements (Regulation (EC) No1107/2009 of the European Parliament and of the Council of 21 October 2009 concerning the placing of plant protection products on the market and repealing council directives 79/117/EEC and 91/414/EE’ 2009). The use of natural products is one of biological control methods in fungal disease control. The method is still not common and investigations are required to find suitable plants that can be used to control pathogenic fungi (El-Mougy Nehal and Abdel-Kader Mokhtar 2007; Kowalska 2011; Remlein-Starosta et al. 2016).
Antifungal activity of essential oils (EOs) such as cinnamon, marigold, basil and spearmint was assessed against pathogens such as Botrytis cinerea Pers., Fusarium gramineraum Corda, Fusarium oxysporum Schltdl. and Sclerotinia sclerotiorum (Lib.) de Bary (Kocic-Tanackov et al. 2011; Al-Taisan et al. 2014; Ghalem 2016). The use of EOs and various plant extracts is aligned with current thinking on the future of agriculture in the EU, and with consumer preference for natural products (Brenes and Roura 2010). The antimicrobial properties of EOs have been widely reported (Dorman and Deans 2000; Mourey and Canillac 2002; Rota et al. 2004). Besides antibacterial properties, EOs or their components have exhibited anti-parasitic (Pandey et al. 2000; Pessoa et al. 2002), and insecticidal (Konstantopoulou et al. 1992; Karpouhtsis et al. 1998) properties. EOs are widely used in various fields, including the cosmetics, pharmaceutical, and food industries (Harkat-Madouri et al. 2015). The constituents of these compounds have antibacterial, viricidal, fungicidal, anti-parasitic, insecticidal, and medicinal properties which are considered to be plants protectants (Bakkali et al. 2008).
Cinnamon oils and extracts showed good antifungal properties against important plant diseases. Wilson et al. (1997) found that among 49 essential oils tested, Cinnamomum zeylanicum J. Presl demonstrated a great antifungal activity against B. cinerea. Cinnamomum zeylanicum commonly known as cinnamon, refers to a tropical evergreen tree as well as a bark that is extracted from the plant. Substances such as cinnamon have been known to the mankind for a very long time. They are universally recognized as harmless and safe for human consumption. Cinnamon powder is considered to have high potential as a biological control agent (Sikes et al. 2005; Xing et al. 2010; Kocić-Tanackov and Dimić 2013).
B. cinerea Pers. Fr. (teleomorph Botryotinia fuckeliana (de Bary) Whetzel) is an economically important phytopathogenic fungus and a cause for a broad spectrum of plants diseases (van Kan 2006; Bolton et al. 2006; Dean et al. 2012). It can cause serious damage in over 200 plant species both in pre- and post-harvest stages and is difficult to control due to various modes of attack and many hosts (Williamson et al. 2007). Very few reports concerning the use of commercially available powdered cinnamon can be found in the literature. Therefore, the aim of the study was to evaluate the potential of commercially available cinnamon powder originating from organic crops used in the liquid form in different concentrations as a control agent against B. cinerea and growth stimulator in both in vitro and in vivo conditions.
Materials and methods
Plant pathogenic fungus preparation
For all tests B. cinerea strain 2235 (plant source: tomato stem) from the Bank of Plant Pathogens in the Institute of Plant Protection, Poland, collection was used. Pure cultures were maintained on Potato Dextrose Agar, Sigma-Aldrich (PDA) media at 4 °C and sub-cultured at monthly intervals. Ten days old cultures were used for the in vitro experiment.
For the greenhouse and field experiments conidia were harvested from ten days old cultures. The concentration was determined using the Thoma slide and adjusted to 3.1 × 105 ml−1. The resulting suspension was used to inoculate the plants.
Cinnamon suspensions and filtrates preparation
The preparation method was developed by the authors. Commercially available organic cinnamon (C. zeylanicum) powder (manufactured by Dary Natury, Grodzisk Poland and certified by Ekogwarancja PTRE sp. z o.o.) was used to obtain water suspensions and filtrates. For the in vitro experiment modified PDA media was used. The cinnamon suspensions were prepared using warm sterile distilled water (26 °C) and added to PDA media to obtain 0.5 and 1% cinnamon PDA suspensions (0.5 and 1 g of cinnamon added to 20 ml of water and 80 ml of the PDA media, respectively). Later cinnamon water suspensions were filtered through a nylon flour mesh (150 μm) and added to PDA to obtain 0.5 and 1% cinnamon filtrates PDA suspensions (0.5 and 1 g of cinnamon powder added to 20 ml of water, filtrated; 20 ml of the filtrate added to 80 ml of the PDA media). For the greenhouse and field experiments 0.5% water filtrate was used. Five grams of cinnamon was added to 1 l of warm tap water (26 °C) and filtered through a flour mesh. The filtrate was prepared shortly before spraying. Water suspensions and filtrates were chosen due to their practical application mode. The filtrates are clearer and do not cause problems with the foliar spraying using a sprayer with a nozzle, while in the case of a suspension containing powder particles, it is necessary to strain the suspension twice before using a fine mesh and to apply with a coarse nozzle.
In vitro biological control activity evaluation
The evaluation of biocontrol activity of cinnamon water suspensions and filtrates against the growth of B. cinerea mycelium was done (El-Mougy Nehal et al. 2004). Disks (0.5 cm diameter each) from pure cultures of the pathogenic fungus were placed in the middle of a 90 mm Petri dish containing PDA with or without the addition of cinnamon water suspensions and filtrates in five replications. Fungi were grown at 23 °C. Mean colony diameter (mm) was measured after 3 and 6 days (i.e. until a control Petri dish was fully covered by the mycelium; the maximum growth was 85 mm – the diameter of the dish excluding the diameter of the initial culture).
Plant development evaluation in greenhouse conditions
Experiments were performed in pots according to methods developed by the authors. Ten tomato plants (Ozarowska variety) in the 3–4 leaf phase were planted into each pot. Each repetition consisted of five pots. Altogether five repetitions were made. Later, plants were inoculated by foliar spray with a water suspension of B. cinerea spores (3.1 × 105 ml−1), 1 ml per plant. Three and six days after inoculation water filtrates of cinnamon at 0.5% was sprayed on the plants surface. The control plants were: a) in the first control group, inoculated with B. cinerea and sprayed with water; and b) in the second control group, not inoculated and sprayed with cinnamon water filtrates. After an additional 5 days, the foliage lesions were assessed (the presence of B. cinerea was verified microscopically). The impact of spraying with cinnamon on plants was evaluated as the above ground fresh weight of plants collected after 3 and 6 weeks of cultivation.
Plant development evaluation in field conditions
The field trial was carried out on a certified organic farm according to methods developed by the authors. Three field tomato varieties (Agro, Country and Hamlet) were used. Seedlings were planted into soil in June, at the 5-leaf phase, with five plants from each cultivar used in each repetition. Altogether four repetitions were made for each cultivar. Six sprayings of water cinnamon filtrates (0.5%) were performed at 4–5 day intervals. The controls were not treated with cinnamon filtrates. The number of leaves and branches was assessed after one and three months of planting to evaluate plants development.
A one-way analysis of variance (ANOVA) was used to test significance with Tukey-Kramer’s post hoc test for multiple comparisons. A p value <0.05 was accepted as statistically significant.
Results and discussion
The influence of cinnamon water suspensions and filtrates on an average lesion diameter [mm] of B. cinerea measured on day 3 and 6 after beginning of in vitro tests
Treatment (cinnamon formulation)
3 days after beginning of test
6 days after beginning of test
Lesion diameter, mm
Reduction of lesion, %
Lesion diameter, mm
Reduction of lesion, %
16.10 ± 1.29a
85.00 ± 0.00a
0.5% water suspension
10.10 ± 1.43a
76.90 ± 18.11a
1% water suspension
5.80 ± 0.84b
85.00 ± 0.00a
0.5% water filtrate
13.70 ± 3.63a
38.80 ± 8.06b
1% water filtrate
8.40 ± 0.65b
15.80 ± 2.02c
The average fresh weight (g) of tomato plant inoculated with B. cinerea and treated with 0.5% cinnamon water filtrates in the pot experiment
Number of weeks of tomato cultivation in pots
Control (water spraying)
Non-inoculated plants sprayed with cinnamon filtrate
Inoculated plants sprayed with tap water
Inoculated plants sprayed with cinnamon filtrate
Tomato fresh weight after 3 weeks,
2,14 ± 0.17a
2,09 ± 0.11a
1,71 ± 0.14a
1,85 ± 0.14a
Tomato fresh weight after 6 weeks
12.83 ± 0.87b
17.17 ± 1.07a
7.83 ± 0.62c
10.50 ± 1.12b
The influence of cinnamon water filtrate (0.5%) on vegetative development of tomato plants after 1 month (July) and 3 months (September) of application under field conditions
Control (water spraying)
Cinnamon water suspension spraying
Number of leaves
12.6 ± 0.58a
11.6 ± 0.08b
11.0 ± 0.71b
11.3 ± 0.55b
12.2 ± 0.50a
14.0 ± 0.71a
Number of branches
14.3 ± 0.58
17.8 ± 1.62a
12.7 ± 1.10b
16.5 ± 0.55a
18.0 ± 1.65a
15.2 ± 0.82a
According to other studies, both in vitro and in planta, essential oils derived from cinnamon proved to have bio-control properties against many pathogens (Siripornvisal et al. 2009; Hyldgaard et al. 2012). Shabana et al. (2015) tested cinnamon essential oil against B. cinerea in vitro and noted a significant suppression of the pathogen growth up to 7 days, comparable to the chemical fungicide Topsin M70-WP. Allam et al. (2017) noted that higher concentration of cinnamon completely inhibited the mycelial growth of B. cinerea in vitro. The antifungal potential of cinnamon was confirmed for others pathogens by other authors (Barrera-Necha et al. 2009; Xing et al. 2014; Sarkhosh et al. 2018). The significant antifungal activity of cinnamon oil (both in vitro and in vivo) against various Fusarium species which was proportional to its concentration was shown. In the study by Horváth et al. (2013) the cinnamon oil effectively inhibited mycelial growth of Fusarium head blight of winter wheat in vitro. Jiang et al. (2013), Al-Taisan et al. (2014) and Moraes et al. (2018) examined the inhibitory effects of cinnamon against S. sclerotiorum among other essential oils and microelements in in vitro experiments and as soil application. All concentrations of cinnamon completely inhibited the mycelial growth of the fungus. According to Moraes et al. (2018) the result was comparable to a commercial fungicide (thiophanate-methyl +chlorothalonil). Ojaghian et al. (2014) proved that crude extracts of cinnamon are able to reduce in vitro mycelial growth, sclerotial myceliogenic and carpogenic germination of S. sclerotiorum. In other studies, during the liquid bioassay, C. zeylanicum was fungicidal against pathogens isolated from banana, including Colletotrichum musae (Berk. & M.A. Curtis), Lasiodiplodia thebromae (Pat.) Griffon & Maubl., and Fusarium proliferatum (Matsush.) Nirenberg ex Gerlach & Nirenberg (Ranasinghe et al. 2002); exerted antifungal activity towards Oidium murrayae Hosag., U. Braun & Rabindran (Chu et al. 2006) and inhibited conidial germination of Colletotrichum gloesporioides Penz.) Penz. & Sacc. (Barrera-Necha et al. 2008). In in vitro experiments it was found to have a good mycelial inhibition of the corn rot F. oxysporum f.sp. gladioli (Massey) (Barrera-Necha et al. 2009) and to be highly effective against the growth of Rhizoctonia solani Kühn (Nguyen et al. 2009), as well as to have excellent antifungal activity against early blight of tomato Alternaria solani Sorauer (Yeole et al. 2014). The investigations of Wang et al. (2014) showed that cinnamon microemulsions had a high in vivo control activity against gray mould of pears B. cinerea. The results obtained by the authors also confirmed activity of cinnamon towards control of grey mould in all experimental areas. In presented research water filtrates of cinnamon were more efficient than water suspensions, this fact has two implications, i) it is more applicable to use and, ii) water filtrates should be used in future tests including also chemical analysis of compounds. So far in planta studies on cinnamon as a bio-control agent against B. cinerea mainly reported its effectiveness on post-harvest products. It applied to such plants as pears (Wang et al. 2014), citrus fruit (Bouchra et al. 2003), peppers and tomatoes (Kong et al. 2016). In most cases cinnamon oil was used. It had no significant negative effect on plant product qualities such as firmness and colour. The control for decay incidence and lesion diameter was reported to be promising.
While existing literature focuses on antifungal properties of cinnamon, the authors have noticed a significant positive effect of cinnamon filtrate spraying on plant branching in the field experiment. In addition to the fact that the antifungal, antibacterial and pest deterring properties of cinnamon improved the general well-being of plants and thus enabled improved growth and branching, there might be another process undergoing on a different level. Therefore, further studies are needed to determine the cause.
To conclude, in laboratory conditions cinnamon water filtrates were more effective against B. cinerea than water suspensions and based on literature it seems to be as effective as cinnamon oil. Further studies are required to recognise the mechanisms of cinnamon improving plants growth and development. Cinnamon water filtrates have also proven to be effective in in planta experiments, including both greenhouse and field tomatoes, where the stimulating effect on plant development and plant health was confirmed. Formulation of water suspensions and its water filtrates were chosen because of its practical application of being used by growers, while the use of cinnamon oil can be difficult in practise. The inhibitory effect of cinnamon water filtrates may be used for practical application provided the treatments are carried out several times at 4–5 day intervals. This frequency of treatments is sufficient to maintain the inhibitory effect on the developing pathogen. This conclusion has practical implications especially for organic growers who have less plant protection products to use than the farmers growing crops the conventional way.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Research involving human participants and/or animals
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