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In vivo and in vitro screening for resistance against Penicillium allii in garlic accessions

  • María C. Salinas
  • Pablo F. CavagnaroEmail author
Article
  • 17 Downloads

Abstract

Garlic blue mold disease, caused by Penicillium allii, is responsible for important economic losses produced yearly in garlic field crops and in postharvest storage. The identification of genetic resistance sources may help in the management of this disease. The present study investigated in vivo and in vitro antifungal effects of a genetically-diverse garlic collection against P. allii in two growing seasons. Cloves of garlic accessions were inoculated with P. allii conidia, and lesion area and sporulation of the fungus in the clove were estimated as a means to characterize the accessions response to the infection. Significant and continuous variation was found among the accessions (p < 0.001), suggesting a quantitative mode of resistance against P. allii in this garlic collection. ‘Castaño’ and ‘Peteco’ were consistently the most resistant and susceptible genotypes, respectively. Culture media of the fungus supplemented with crude garlic extracts (CGEs) revealed significant inhibition of P. allii growth in vitro by all the garlic accessions, with fungal-growth inhibition being dose-dependent up to a concentration of 20–26% (v/v), beyond which the CGEs became fungicidal. These data indicate that garlic bulbs have antifungal compounds, which at high concentration can be fungicidal for P. allii. CGEs of ‘Castaño’ and a few other accessions were consistently the more inhibitory extracts. Total phenolics and pyruvate (an estimator of the total thiosulfinates content) concentration in the garlic bulbs was not associated with P. allii resistance in vivo, but their content in the CGEs correlated significantly and negatively with the fungal growth in vitro (r = −0.38 to −0.72), suggesting that these compounds have antifungal effect, but their content is not the main factor conditioning P. allii resistance in vivo.

Keywords

Allium sativum Blue mold Penicillium allii Disease resistance Thiosulfinates Phenolic compounds 

Notes

Acknowledgements

The authors gratefully acknowledge Jorge Valdez and Jose Luis Burba (INTA La Consulta) for providing the P. allii isolate and the garlic materials used in this study, respectively. This work was supported by grant A008 from the ‘Secretaría de Ciencia, Técnica y Posgrado’ of the National University of Cuyo, Mendoza, Argentina.

Compliance with ethical standards

The authors declare that the research complies with ethical standards.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  1. 1.National Council of Scientific and Technical Research (CONICET)MendozaArgentina
  2. 2.National Institute of Agricultural Technology (INTA) – E.E.A. La ConsultaMendozaArgentina
  3. 3.Faculty of Agricultural SciencesNational University of CuyoMendozaArgentina

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