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European Journal of Plant Pathology

, Volume 155, Issue 3, pp 871–880 | Cite as

Cell wall degrading enzymes and their impact on Fusarium proliferatum pathogenicity

  • Anwar H. Sharafaddin
  • Younis K. Hamad
  • Mahmoud H. El_Komy
  • Yasser E. Ibrahim
  • Arya Widyawan
  • Younes Y. Molan
  • Amgad A. SalehEmail author
Article
  • 259 Downloads

Abstract

Fusarium proliferatum is one of the most pathogenic Fusarium species on date palm worldwide. We evaluated the colonization ability of 42 F. proliferatum strains collected from date palm trees with disease symptoms on detached leaflets of cvs Sheeshee and Sukkary. Cell wall degrading enzyme (CWDE) production by these strains was assessed with multiple relative enzyme activity (RA) indices. The ability of F. proliferatum strains to colonize leaflet cuttings varied, and there were significant differences (P < 0.05) in susceptibility between the Sheeshee and Sukkary cvs. All F. proliferatum strains tested could hydrolyze carboxymethylcellulose (CMC), citrus pectin and starch, with different capabilities. RA indices calculated from direct assessment of halo zones were consistent with fungal strain colonization ability, but ratio-based RA indices were not. Based on RA4 index of cellulases, F. proliferatum strains were divided into three groups. The first group (33% of strains) showed very strong reaction in hydrolysing CMC, the second group (62% of strains) showed strong reaction in hydrolysing CMC, and the third group (5%) showed moderate reaction in hydrolysing CMC. Most strains had moderate pectin-hydrolysis activities. Starch-hydrolysis activities ranged from weak to strong. Overall, there was a positive correlation between cellulase production and a strain’s colonization ability. Thus, CWDEs produced by F. proliferatum play a role in the colonization of date palm leaflets and hence may be important pathogenicity factors.

Keywords

Colonization ability Hydrolysis Cellulases Pectinases Amylases 

Notes

Acknowledgements

The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding this work through the research group no. RG-1440-001.

Compliance with ethical standards

Ethical statement

I testify, along with the co-authors, that the submitted manuscript has not been either submitted to more than one journal for simultaneous consideration or published previously in whole or in part elsewhere. All authors whose names appear on the manuscript have contributed sufficiently to the present study and therefore share collective responsibility and accountability for the results.

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

The presented work does not contain any studies with human participants or animals.

Informed consent

All the authors on the presented work agreed to submit this manuscript to be considered for publication in the European Journal of Plant Pathology.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  • Anwar H. Sharafaddin
    • 1
  • Younis K. Hamad
    • 1
    • 2
  • Mahmoud H. El_Komy
    • 1
    • 3
  • Yasser E. Ibrahim
    • 1
    • 3
  • Arya Widyawan
    • 1
  • Younes Y. Molan
    • 1
  • Amgad A. Saleh
    • 1
    • 4
    Email author
  1. 1.Department of Plant Protection, College of Food and Agriculture SciencesKing Saud UniversityRiyadhKingdom of Saudi Arabia
  2. 2.Plant Pathology Department, Faculty of AgricultureAlexandria UniversityAlexandriaEgypt
  3. 3.Plant Pathology InstituteAgriculture Research CenterGizaEgypt
  4. 4.Agricultural Genetic Engineering Research InstituteAgriculture Research CenterGizaEgypt

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