Detection and isolation of Ceratocystis fimbriata in mango trees on semi-selective medium
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Ceratocystis wilt in mango, caused by Ceratocystis fimbriata, is a problem in Brazil, Oman and Pakistan. There is a lack of knowledge regarding this disease, including field detection of the pathogen. This study evaluated the detection of C. fimbriata through carrot baits and a semi-selective medium of stem-inoculated 1-year-old mango plants in a greenhouse and naturally infected 15-year-old mango trees in a commercial orchard. The pathogen was detected with the carrot baits from samples collected at the inoculation point in some greenhouse plants but not from the field trees. C. fimbriata was detected using the semi-selective medium up to 16 cm below the inoculation site of the greenhouse plants, in most of the samples taken from the lesion sites in the field trees, and in one of ten asymptomatic trees that were sampled from the orchard. We concluded that semi-selective medium is an effective and rapid method for diagnosis of C. fimbriata. This is useful for epidemiological studies and more importantly, to manage Ceratocystis wilt in the field.
KeywordsMango wilt Wood detection Mangifera indica Carrot baits
We are grateful to Vale for funding this project and supporting this work.
Compliance with ethical standards
I, Renata Aparecida dos Santos Brito, agree with all the information contained in this manuscript. This work is being submitted only in the European Journal of Plant Pathology, with exclusivity. No other information has been published previously. All information and results are fully described. No data has been manufactured or manipulated and no data or text has been copied from published works. All information in the literature is cited. Tests with participation of humans and animals was not used for this research. All co-authors are aware of the submission of this manuscript and there is no conflict of interest. The authors of this scientific paper contributed sufficiently and, therefore, have responsibility for the results showed.
- Baker, C. J., & Harrington, T. C. (2004). Ceratocystis fimbriata. Crop protection compendium. In Kew. Surrey: CABI Publishing.Google Scholar
- Laia, M. L., Alfenas, A. C., & Harrington, T. C. (1999). Isolation, detection in soil, and inoculation of Ceratocystis fimbriata, causal agent of wilting, die-back and canker in eucalyptus. In Proceedings of the 12th biennial conference of the Australasian plant pathology society (p. 77).Google Scholar
- Masood, A., Saeed, S., Erbilgin, N., & Jung Kwon, Y. (2010). Role of stressed mango host conditions in attraction of and colonization by the mango bark beetle Hypocryphalus mangiferae Stebbing (Coleoptera: Curculionidae: Scolytinae) and in the symptom development of quick decline of mango trees in Pakistan. Entomological Research, 40(6), 316–327.CrossRefGoogle Scholar
- Moller, W. J., & DeVay, J. E. (1968). Carrot as a species-selective isolation medium for Ceratocystis fimbriata. Phytopathology, 58, 123–124.Google Scholar
- Moutia, Y., & Saumtally, S. (2001). Detection from soil and distribution of Ceratocystis paradoxa Moreau, causal agent of the pineapple disease of sugarcane. Food and Agricultural Research Council (pp. 75–82).Google Scholar
- Rossetto, C. J., & Ribeiro, I. J. A. (1990). Seca-da-mangueira XII. Recomendações de controle. Revista de Agricultura, 65, 173–180.Google Scholar
- Terao, D., Batista, D. C., & Ribeiro, I. J. A. (2016). Doenças da Mangueira. In L. Amorin, J. A. Rezende, A. Bergamin Filho, & L. E. A. Camargo (Eds.), Manual de Fitopatologia Volume 2. Doenças de Plantas Cultivadas (5th ed., pp. 523–543). Ouro Fino-MG: Agronômica Ceres.Google Scholar