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Histopathology of infection and colonisation of Elsinoë ampelina on grapevine leaves

  • Zélia Valente Braga
  • Ricardo Feliciano dos Santos
  • Lilian AmorimEmail author
  • Beatriz Appezzato-da-GlóriaEmail author
Article
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Abstract

Elsinoë ampelina, the causal agent of grapevine anthracnose, infects all aboveground succulent parts of the plant, causing serious yield losses. There are no histopathological reports on this pathogen interaction with grapevine tissues. This study aimed at understanding the infection and colonisation processes of E. ampelina on Vitis labrusca cv. Niagara Rosada leaves by light, transmission and scanning microscopy. From one to five germ tubes per conidia were formed during conidia germination, predominantly one or two. Cuticle degradation was observed on plant surface underneath germ tubes and appressorium-like structures were rare. Monilioid and branched hyphae were observed 48 h post inoculation. Direct penetration occurred only on the adaxial leaf surface due to high trichome density on the abaxial leaf surface and conidia trapped on trichomes were frequently observed. The colonisation process was similar in the leaf blade, veins and petiole with the formation of inter- and intracellular hyphae. Phenolic compounds accumulated in infected areas; however, they did not prevent the colonisation progress. Tissues of foliar blade were totally changed by cell collapse and conidiogenous cells were observed on the surface of necrotic lesions. Ascus-like structures were observed within deeply unstructured tissues of leaf veins and necrotic areas of the petiole.

Keywords

Vitis labrusca Sphaceloma ampelinum Necrotic symptoms Direct penetration Grapevine black spot 

Notes

Acknowledgements

We would like to FAPESP for financial support (project n° 2013/24003-9), NAP-MEPA, ESALQ/USP for electron microscope facilities. This study was also financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.

Compliance with ethical standards

This manuscript is original and complies to the ethical rules applicable for this journal.

Conflict of interest

The authors do not present any actual or potential conflict of interests.

Supplementary material

10658_2019_1721_MOESM1_ESM.docx (929 kb)
ESM 1 (DOCX 929 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  • Zélia Valente Braga
    • 1
  • Ricardo Feliciano dos Santos
    • 2
  • Lilian Amorim
    • 2
    Email author
  • Beatriz Appezzato-da-Glória
    • 1
    Email author
  1. 1.Departamento de Ciências Biológicas, Escola Superior de Agricultura “Luiz de Queiroz”Universidade de São PauloPiracicabaBrazil
  2. 2.Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura “Luiz de Queiroz”Universidade de São PauloPiracicabaBrazil

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