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Proteomic analysis of the tripartite interaction between black pepper, Trichoderma harzianum and Phytophthora capsici provides insights into induced systemic resistance mediated by Trichoderma spp.

  • P. Umadevi
  • M. AnandarajEmail author
Article
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Abstract

Trichoderma harzianum (MTCC5179) is the biocontrol agent in the black pepper (Piper nigrum.L) production system against the destructive pathogen Phytophthora capsici which causes foot and root rot. We employed label-free quantitative proteomics to study the T. harzianum mediated induced systemic response in this system. We studied the defence response in leaves in T. harziznum primed plant roots which are also infected with P. capsici. The pattern of interactions was studied as black pepper × T. harzianum (two-way), black pepper × P. capsici (two-way) and black pepper × T. harzianum × P. capsici (three-way). The proteins induced only in the three-way interaction were identified as Trichoderma induced resistance proteins. Eighteen reactive oxygen species-related proteins and 22 defence-related proteins were identified as marker proteins. Apart from these groups, the ethylene synthesis, isoflavanoid pathway and lignin synthesis proteins were found to be enhanced. We report the early induced systemic resistance in leaves after Trichoderma priming at roots (72, and 96 h after interaction) against Phytophthora capsici after 12 and 24 h of infection at roots. The peptides/proteins from this study will serve as important marker peptides/proteins for the induced systemic resistance in plants by Trichoderma.

Keywords

Proteomics Peptides ROS related proteins Defense-related proteins Phytophthora capsici T-ISR 

Notes

Acknowledgments

The authors acknowledge with thanks the financial support from ICAR-Outreach programme on Phytophthora, Fusarium and Ralstonia diseases of horticultural and field crops (PhytoFuRa). The mass spectrometry performed by C-CAMP, Bangalore, is gratefully acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Involvement of human participants and /or animals

The present research did not involve any experimentation on humans or animals.

Informed consent

Authors are ready to provide any additional information of the present study to the readers.

Supplementary material

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Supplementary file 1 (XLSX 13 kb)
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Supplementary file 4 (DOCX 1826 kb)

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© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  1. 1.ICAR- Indian Institute of Spices ResearchKozhikodeIndia

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