Advertisement

Distribution and molecular characterization of Citrus leaf blotch virus from Actinidia in Shaanxi province, China

  • Huan Liu
  • Shuang Song
  • Wei Wu
  • Weili Mi
  • Chuan Shen
  • Bixin Bai
  • Yunfeng WuEmail author
Article
  • 91 Downloads

Abstract

Kiwi fruit (Actinidia spp.) is one of the most important fruit crops in the world. Shaanxi is the largest kiwi fruit growing area in China. In this study, field investigation and virus detection showed that Citrus leaf blotch virus (CLBV) commonly occurred in Shaanxi Province and the average incidence rate was 28.53%. In total, some 60 complete nucleotide sequences of movement protein (MP) and coat protein (CP) genes of CLBV were obtained from different regions of Shaanxi. Phylogenetic analysis showed that CLBV had obvious host specialization. The study of CLBV isolates in kiwi fruit preliminarily revealed that CLBV was not significantly associated with the host’s geographic location. The results provide important information for further clarifying the distribution and genetic diversity of CLBV in regions of kiwi fruit production in Shaanxi and lay the foundation for in-depth study of the molecular characteristics and epidemiology of the virus.

Keywords

Kiwi fruit Citrus leaf blotch virus Distribution Molecular characterization 

Notes

Funding

This study was supported by key research and development plan in shaanxi province(2018ZDXM-NY-058) and the 111 Project from the Education Ministry of China (B07049).

Compliance with ethical standards

Conflict of interest

No conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication. I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part. All the authors listed have approved the manuscript that is enclosed.

Supplementary material

10658_2019_1677_MOESM1_ESM.docx (26 kb)
ESM 1 (DOCX 26 kb)
10658_2019_1677_MOESM2_ESM.docx (2.6 mb)
ESM 2 (DOCX 2666 kb)

References

  1. Adams, M. J., Candresse, T., Hammond, J., Kreuze, J. F., Martelli, G. P., Namba, S., Pearson, M. N., Ryu, K. H., Saldarelli, P., & Yoshikawa, N. (2012). Family Betaflexiviridae. In A. M. Q. King, M. J. Adams, E. B. Carstens, & E. J. Lefkowitz (Eds.), Virus taxonomy: Ninth report of the international committee on taxonomy of viruses (pp. 920–941). London: Elsevier Academic Press.Google Scholar
  2. Aguero, J., Vives, M. C., Velazquez, K., Ruiz-Ruiz, S., Juarez, J., Navarro, L., Moreno, P., & Guerri, J. (2013). Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus. Molecular Plant Pathology, 14, 610–616.CrossRefGoogle Scholar
  3. Blouin, A. G., Pearson, M. N., Chavan, R. R., Woo, E. N. Y., Lebas, B. S. M., Veerakone, S., Ratti, C., Biccheri, R., MacDiarmid, R. M., & Cohen, D. (2013). Viruses of kiwifruit (Actinidia species). Journal of Plant Pathology, 95, 221–235.Google Scholar
  4. Blouin, A. G., Biccheri, R., Khalifa, M. E., Pearson, M. N., Pollini, C. P., Hamiaux, C., & Ratti, C. (2018). Characterization of Actinidia virus 1, a new member of the family Closteroviridae encoding a thaumatin-like protein. Archives of Virology, 163, 229–234.CrossRefGoogle Scholar
  5. Cao, M. J., Yu, Y. Q., Tian, X., Yang, F. Y., Li, R. H., & Zhou, C. Y. (2017). First report of Citrus leaf blotch virus in lemon in China. Plant Disease, 101, 1561–1561.CrossRefGoogle Scholar
  6. Chavan, R. R., Blouin, A. G., Cohen, D., & Pearson, M. N. (2013). Characterization of the complete genome of a novel citrivirus infecting Actinidia chinensis. Archives of Virology, 158, 1679–1686.CrossRefGoogle Scholar
  7. Ferrante, P., & Scortichini, M. (2014). Frost promotes the pathogenicity of Pseudomonas syringae pv. Actinidiae in Actinidia chinensis and A. deliciosa plants. Plant Pathology, 63, 12–19.CrossRefGoogle Scholar
  8. Gress, J. C., Smith, S., & Tzanetakis, I. E. (2017). First report of Citrus leaf blotch virus in Peony in the USA. Plant Disease, 101, 637–637.CrossRefGoogle Scholar
  9. Guardo, M., Sorrentino, G., Marietta, T., & Caruso, A. (2007). First report of Citrus leaf blotch virus on kumquat in Italy. Plant Disease, 91, 1054–1054.CrossRefGoogle Scholar
  10. Guardo, M., Potere, O., Castellano, M. A., Savino, V., & Caruso, A. (2009). A new herbaceous host of Citrus leaf blotch virus. Journal of Plant Pathology, 91, 485–488.Google Scholar
  11. Heath, L., van der Walt, E., Varsani, A., & Martin, D. P. (2006). Recombination patterns in aphthoviruses mirror those found in other picornaviruses. Journal of Virology, 80, 11827–11832.CrossRefGoogle Scholar
  12. Li, Z., Kondo, H., Andika, I. B., Liu, P., Sun, L., & Wu, Y. (2016a). Identification of genome recombination among apple stem pitting virus isolates. Journal of Plant Pathology, 98, 595–601.Google Scholar
  13. Li, Y., Zhong, Y., Huang, K., & Cheng, Z. M. (2016b). Genomewide analysis of NBS-encoding genes in kiwi fruit (Actinidia chinensis). Journal of Genetics, 95, 997–1001.CrossRefGoogle Scholar
  14. Li, L., Pan, H., Chen, M. Y., Zhang, S. J., & Zhong, C. H. (2017). First report of anthracnose caused by Colletotrichum gloeosporioides on kiwifruit (Actinidia chinensis) in China. Plant Disease, 101, 2151–2151.CrossRefGoogle Scholar
  15. Li, P., Li, M., Zhang, S., Wang, J., Yang, F. Y., Cao, M. J., & Li, Z. A. (2018). Complete genome sequences of four isolates of Citrus leaf blotch virus from citrus in China. Journal of Integrative Agriculture, 17, 712–715.CrossRefGoogle Scholar
  16. Liu, P., Li, Z., Song, S., & Wu, Y. (2014). Molecular variability of apple chlorotic leaf spot virus in Shaanxi, China. Phytoparasitica, 42, 445–454.CrossRefGoogle Scholar
  17. Marais, A., Faure, C., Mustafayev, E., & Candresse, T. (2015). Characterization of new isolates of apricot vein clearing-associated virus and of a new Prunus-infecting virus: Evidence for recombination as a driving force in Betaflexiviridae evolution. PLoS One, 10, e0129469.CrossRefGoogle Scholar
  18. Martelli, G. P., Adams, M. J., Kreuze, J. F., & Dolja, V. V. (2007). Family Flexiviridae: A case study in virion and genome plasticity. Annual Review of Phytopathology, 45, 73–100.CrossRefGoogle Scholar
  19. Veerakone, S., Liefting, L. W., Tang, J., & Ward, L. I. (2018). The complete nucleotide sequence and genome organisation of a novel member of the family Betaflexiviridae from Actinidia chinensis. Archives of Virology, 163, 1367–1370.CrossRefGoogle Scholar
  20. Vives, M. C., Galipienso, L., Navarro, L., Moreno, P., & Guerri, J. (2001). The nucleotide sequence and genomic organization of Citrus leaf blotch virus: Candidate type species for a new virus genus. Virology, 287, 225–233.CrossRefGoogle Scholar
  21. Vives, M. C., Galipienso, L., Navarro, L., Moreno, P., & Guerri, J. (2002). Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus. Virology, 295, 328–336.CrossRefGoogle Scholar
  22. Wang, K. X., Xie, Y. L., Yuan, G. Q., Li, Q. Q., & Lin, W. (2015). First report of root and collar rot caused by Phytopythium helicoides on kiwifruit (Actinidia chinensis). Plant Disease, 99, 725–726.CrossRefGoogle Scholar
  23. Wang, Y. X., Hong, N., Wang, G. P., Yang, Z. K., Wang, L. P., & Li, L. (2016a). First report of the Tospovirus tomato necrotic spot associated virus infecting kiwifruit (Actinidia sp.) in China. Plant Disease, 100, 2539.CrossRefGoogle Scholar
  24. Wang, J., Zhu, D., Tan, Y., Zong, X., Wei, H., & Liu, Q. (2016b). First report of Citrus leaf blotch virus in sweet cherry. Plant Disease, 100, 1027–1027.CrossRefGoogle Scholar
  25. Wang, D., Liu, X. X., Li, T. T., Li, Q., Liu, Y., Gong, G. S., & Yang, H. (2018a). First report of cucumber mosaic virus infection in kiwifruit (Actinidia chinensis) in China. Plant Disease, 102, 1180–1180.CrossRefGoogle Scholar
  26. Wang, Y. X., Zhuang, H. Q., Yang, Z. K., Wen, L. H., Wang, G. P., & Hong, N. (2018b). Molecular characterization of an apple stem grooving virus isolate from kiwifruit (Actinidia chinensis) in China. Canadian Journal of Plant Pathology, 40, 76–83.CrossRefGoogle Scholar
  27. Wu, B. L., Blanchard-Letort, A., Liu, Y., Zhou, G. H., Wang, X. F., & Elena, S. F. (2011). Dynamics of molecular evolution and Phylogeography of barley yellow dwarf virus-PAV. PLoS One, 6.Google Scholar
  28. Zheng, Y. Z., Navarro, B., Wang, G. P., Wang, Y. X., Yang, Z. K., Xu, W. X., Zhu, C. X., Wang, L. P., Di Serio, F., & Hong, N. (2017). Actinidia chlorotic ringspot-associated virus: A novel emaravirus infecting kiwifruit plants. Molecular Plant Pathology, 18, 569–581.CrossRefGoogle Scholar
  29. Zhu, C. X., Wang, G. P., Zheng, Y. Z., Yang, Z. K., Wang, L. P., Xu, W. N., & Hong, N. (2016). RT-PCR detection and sequence analysis of coat protein gene of Citrus leaf blotch virus infecting kiwifruit trees (in Chinese). Acta Phytopathologica Sinica, 46, 11–16.Google Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  • Huan Liu
    • 1
  • Shuang Song
    • 2
  • Wei Wu
    • 3
  • Weili Mi
    • 1
  • Chuan Shen
    • 1
  • Bixin Bai
    • 1
  • Yunfeng Wu
    • 1
    Email author
  1. 1.State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Key Laboratory of Crop Pest Integrated Pest Management on Crop in Northwestern Loess Plateau, Ministry of AgricultureNorthwest A&F UniversityYanglingChina
  2. 2.Department of Plant Protection, College of AgricultureNortheast Agricultural UniversityHarbinChina
  3. 3.College of Life SciencesNorthwest A&F UniversityYanglingChina

Personalised recommendations