Distribution and molecular characterization of Citrus leaf blotch virus from Actinidia in Shaanxi province, China

  • Huan Liu
  • Shuang Song
  • Wei Wu
  • Weili Mi
  • Chuan Shen
  • Bixin Bai
  • Yunfeng WuEmail author


Kiwi fruit (Actinidia spp.) is one of the most important fruit crops in the world. Shaanxi is the largest kiwi fruit growing area in China. In this study, field investigation and virus detection showed that Citrus leaf blotch virus (CLBV) commonly occurred in Shaanxi Province and the average incidence rate was 28.53%. In total, some 60 complete nucleotide sequences of movement protein (MP) and coat protein (CP) genes of CLBV were obtained from different regions of Shaanxi. Phylogenetic analysis showed that CLBV had obvious host specialization. The study of CLBV isolates in kiwi fruit preliminarily revealed that CLBV was not significantly associated with the host’s geographic location. The results provide important information for further clarifying the distribution and genetic diversity of CLBV in regions of kiwi fruit production in Shaanxi and lay the foundation for in-depth study of the molecular characteristics and epidemiology of the virus.


Kiwi fruit Citrus leaf blotch virus Distribution Molecular characterization 



This study was supported by key research and development plan in shaanxi province(2018ZDXM-NY-058) and the 111 Project from the Education Ministry of China (B07049).

Compliance with ethical standards

Conflict of interest

No conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication. I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part. All the authors listed have approved the manuscript that is enclosed.

Supplementary material

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  • Huan Liu
    • 1
  • Shuang Song
    • 2
  • Wei Wu
    • 3
  • Weili Mi
    • 1
  • Chuan Shen
    • 1
  • Bixin Bai
    • 1
  • Yunfeng Wu
    • 1
    Email author
  1. 1.State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Key Laboratory of Crop Pest Integrated Pest Management on Crop in Northwestern Loess Plateau, Ministry of AgricultureNorthwest A&F UniversityYanglingChina
  2. 2.Department of Plant Protection, College of AgricultureNortheast Agricultural UniversityHarbinChina
  3. 3.College of Life SciencesNorthwest A&F UniversityYanglingChina

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