Variable ITS-copy number at different developmental stages of Meloidogyne hapla and M. chitwoodi

  • Everaldo Antônio LopesEmail author
  • David Mark Roberts
  • Vivian Carol Blok


Quantitative polymerase chain reaction (qPCR) can be used for the accurate quantification of plant-parasitic nematodes from soil samples. Unlike the traditional Baermann funnel and centrifugal flotation techniques followed by visual enumeration, all developmental stages of plant-parasitic nematodes can be detected and quantified by using qPCR. However, little is known about the amount of DNA between different stages of plant-parasitic nematodes. Here, we show that ITS-copy number varies with developmental stage of Meloidogyne chitwoodi and M. hapla. The number of copies of ITS was lower in cell-stage eggs (51 ± 7.4 for M. hapla and 31 ± 6.0 for M. chitwoodi) than in second-stage juveniles (1345 ± 130.6 for M. hapla and 1036 ± 140.3 for M. chitwoodi) and females (19,508 ± 3367.3 for M. hapla and 9049 ± 316.7 for M. chitwoodi).


Number of copies qPCR Quantification Real-time PCR Root-knot nematode 



The authors are grateful for FERA (The Food and Environment Research Agency, United Kingdom) for providing nematode inoculum. E.A. Lopes thanks CNPq (Proc. 304663/2014-0) and The James Hutton Institute for Post-Doctoral Training.


This study was funded by CNPq (PDE 233650/2014–8) and the Rural and Environment Science and Analytical Services (RESAS) Division of the Scottish Government.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Authors and Affiliations

  1. 1.Universidade Federal de ViçosaRio ParanaíbaBrazil
  2. 2.The James Hutton InstituteDundeeUK

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