Isolation and identification of a Bacillus subtilis HZ-72 exhibiting biocontrol activity against flax seedling blight
- 121 Downloads
Seedling blight caused by Rhizoctonia solani is a serious soil-borne disease on flax. In this study, we isolated a bacterial strain HZ-72 from the rhizosphere soil of flax with obvious inhibitory effect on R. solani and other six plant fungal pathogens. Strain HZ-72 was identified as Bacillus subtilis based on morphological, physiological, biochemical characteristics and 16S rDNA sequence analysis. In greenhouse experiments, the control efficiency of strain HZ-72 reached 83.34%. Additionally, in vitro assays indicated that cell wall-degrading enzymes such as protease and cellulase, volatile compounds, proteins and lipopeptides produced by strain HZ-72 all contributed to its antagonistic activity against R. solani. To our knowledge, this is the first report on the use of a rhizosphere B. subtilis strain as a biocontrol agent for the biocontrol of flax seedling blight caused by R. solani.
KeywordsRhizosphere bacteria Bacillus subtilis Flax Rhizoctonia solani Biocontrol
This work was supported by the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2015- IBFC), the Major Scientific and Technological Projects of Hunan Province (2016NK1001), the National Key Research and Development Program of China (2017YFD0200900) and the project for Monitoring and Prevention of Crop Pests, Disease, and Mice from the Ministry of Agriculture (S158).
Compliance with ethical standards
Conflict of interest
The work complies to the ethical standards of this journal. The authors declare that they have no conflict of interest. This research did not involve human and/or animal participants. The manuscript was only submitted to EJPP and not previously published. All authors contributed and agreed to submission to EJPP.
- Buchanan, R. E., & Gibbons, N. E. (1984). Bergey’s manual of determinative bacteriology (8th Chinese ed.). Beijing: Science Press.Google Scholar
- Chauhan, A. K., Maheshwari, D. K., Kim, K., & Bajpai, V. K. (2016). Termitarium-inhabiting Bacillus endophyticus TSH42 and Bacillus cereus TSH77 colonizing Curcuma longa L.: Isolation, characterization, and evaluation of their biocontrol and plant-growth-promoting activities. Canadian Journal of Microbiology, 62(10), 880–892.CrossRefGoogle Scholar
- Dong, X. Z., & Cai, M. Y. (2001). Common bacterial system identification manual. Beijing: Science Press.Google Scholar
- Guo, X., Chen, D. D., Peng, K. S., Cui, Z. W., Zhang, X. J., Li, S., & Zhang, Y. A. (2016). Identification and characterization of Bacillus subtilis from grass carp (Ctenopharynodon idellus) for use as probiotic additives in aquatic feed. Fish & Shellfish Immunology, 52, 74–84.CrossRefGoogle Scholar
- He, J. Q., Wang, J. L., Tang, Y. B., & Wang, R. X. (2005). A technical study on flax seeds treatment with fungicides against Rhizoclonia solanikiikn. China’s Fiber and Products, 27(3), 146–148.Google Scholar
- Jadhav, H. P., Shaikh, S. S., & Sayyed, R. Z. (2017). Role of hydrolytic enzymes of rhizoflora in biocontrol of fungal phytopathogens: An overview. In Rhizotrophs: plant growth promotion to bioremediation (pp. 183–203). Singapore: Springer.Google Scholar
- Kumar, K. V. K., Yellareddygari, S. K., Reddy, M. S., Kloepper, J. W., Lawrence, K. S., Zhou, X. G., Sudini, H., Groth, D. E., Raju, S. K., & Miller, M. E. (2012). Efficacy of Bacillus subtilis MBI 600 against sheath blight caused by Rhizoctonia solani and on growth and yield of rice. Rice Science, 19(1), 55–63.CrossRefGoogle Scholar
- Lane, D. J. (1991). 16S/23S rRNA sequencing. In E. Stackebrandt & M. Goodfellow (Eds.), Nucleic acid techniques in bacterial systematices (pp. 115–175). Chichester: Wiley.Google Scholar
- Yang, X., Liu, L. Y., Guang, F. Z., Li, Z. G., Wu, G. W., Wang, X., Lu, Y., & Chen, H. (2009). Identification of flax Rhizoctonia solani pathogen and medicament selection. Heilongjiang Agricultural Sciences in China, 2009(4), 67–68.Google Scholar
- Youssef, S. A., Tartoura, K. A., & Abdelraouf, G. A. (2016). Evaluation of Trichoderma harzianum and Serratia proteamaculans effect on disease suppression, stimulation of ROS-scavenging enzymes and improving tomato growth infected by Rhizoctonia solani. Biological Control, 100, 79–86.CrossRefGoogle Scholar
- Zhang, M. J., Li, J. L., Shen, A. R., Tan, S. Y., Yan, Z., Yu, Y. T., Xue, Z. D., Tan, T. M., & Zeng, L. B. (2016). Isolation and identification of Bacillus amyloliquefaciens IBFCBF-1 with potential for biological control of Phytophthora blight and growth promotion of pepper. Journal of Phytopathology, 164(11–12), 1012–1021.CrossRefGoogle Scholar
- Zhang, B., Wang, J. N., Ning, S. Q., Yuan, Q., Chen, X. N., Zhang, Y. Y., & Fan, J. F. (2018). Peptides derived from tryptic hydrolysate of Bacillus subtilis culture suppress fungal spoilage of table grapes. Food Chemistry, 239, 520–528. https://doi.org/10.1016/j.foodchem.2017.06.153.CrossRefGoogle Scholar
- Zhao, S., Li, J. Y., Liu, F., Yang, W. X., Zhang, N., & Liu, D. Q. (2015). Antagonism of Paenibacillus polymyxa Z-X-225 against pathogen of pricklyash peel ear blight disease. Journal of Plant Protection in China, 42(5), 863–864.Google Scholar
- Zhu, X., Yang, G. A., Wang, X. M., Chen, X. Y., Sun, C. H., Yang, J. Q., Dong, L. M., Chen, G. H., Sheng, J. B., Tian, C. L., & Yang, W. G. (2010). Control efficacy of several agrochemicals to flax Rhizoctonia solani in field. Plant Fiber Sciences in China, 32(6), 323–326.Google Scholar