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European Journal of Plant Pathology

, Volume 153, Issue 3, pp 813–823 | Cite as

Differences in infectivity and pathogenicity of two Plantago asiatica mosaic virus isolates in lilies

  • Masashi Tanaka
  • Martin Verbeek
  • Miki Takehara
  • Khanh Pham
  • Miriam Lemmers
  • Casper Slootweg
  • Tsutomu Arie
  • Ken KomatsuEmail author
Article
  • 76 Downloads

Abstract

Plantago asiatica mosaic virus (PlAMV) is a member of the genus Potexvirus in the family Alphaflexiviridae and has been isolated from a variety of host plants. In particular, PlAMV isolates from ornamental lilies (Lilium spp.) cause necrotic symptoms in these plants, which significantly reduces their commercial value. However, it has not been clear whether PlAMV isolates from other host plants differ in their infectivity and/or pathogenicity to ornamental lilies, and whether growth conditions affect infectivity and pathogenicity. In this study, we inoculated an edible lily species (Lilium leichtlinii) and seven varieties of ornamental lilies with two PlAMV isolates, an isolate from ornamental lily (PlAMV-OL) and an isolate from edible lily (PlAMV-Li1). We found that PlAMV-OL showed higher infection rates and exhibited necrotic symptoms more frequently in lilies than PlAMV-Li1. Moreover, we observed higher infection rates of PlAMV-OL in open field than in greenhouse, and higher rates of necrotic symptoms in autumn test than in spring test, suggesting that growth conditions and season affect infectivity and pathogenicity of PlAMV in lilies. Our study would provide important information for estimating the risk of necrotic disease caused by PlAMV, as well as for cultivation management preventing the occurrence of the disease.

Keywords

Plantago asiatica mosaic virus Ornamental lily Necrosis Infectivity Pathogenicity Environmental effects 

Notes

Acknowledgements

We thank YAMAKI NOEN CORPOLATION for providing lily bulbs and cultivation site. This work was supported by Overseas travel assistance program executed by TUAT president discretionary fund.

Compliance with ethical standards

Informed consent

The paper has not been submitted elsewhere for publication, in whole or in part.

Conflict of interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Ethical approval

This paper does not contain studies on human or animal participants.

Supplementary material

10658_2018_1594_MOESM1_ESM.docx (192 kb)
Figure. S1 Detection of LMoV from lily bulbs used for inoculation test. Amplicons by RT-PCR were analyzed in a 1% agarose gel. M: 1kbp DNA Ladder (New England BioLabs, USA), lane 1: A sample from a LMoV-infected lily plant (positive control), lane 2 to 13: 12 samples from L. ‘Sheila’ bulbs. Arrow indicates amplicons from LMoV (767 bp). (DOCX 192 kb)
10658_2018_1594_MOESM2_ESM.docx (1.8 mb)
Figure. S2 Necrotic symptoms observed in leaves (a) and stems (b) of edible lily inoculated with PlAMV-OL about 2 months post inoculation. (DOCX 1845 kb)
10658_2018_1594_MOESM3_ESM.docx (607 kb)
Figure. S3 Local lesions observed in four leaves of Chenopodium quinoa inoculated with PlAMV-Li1 (a) and PlAMV-OL (b). (DOCX 606 kb)
10658_2018_1594_MOESM4_ESM.docx (19 kb)
Supplementary Table 1 (DOCX 18 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  1. 1.Laboratory of Plant Pathology, Graduate School of AgricultureTokyo University of Agriculture and Technology (TUAT)FuchuJapan
  2. 2.Wageningen University and ResearchWageningenThe Netherlands
  3. 3.Wageningen University and ResearchLisseThe Netherlands
  4. 4.Institute of Global Innovation ResearchTokyo University of Agriculture and TechnologyFuchuJapan

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