European Journal of Plant Pathology

, Volume 153, Issue 3, pp 813–823 | Cite as

Differences in infectivity and pathogenicity of two Plantago asiatica mosaic virus isolates in lilies

  • Masashi Tanaka
  • Martin Verbeek
  • Miki Takehara
  • Khanh Pham
  • Miriam Lemmers
  • Casper Slootweg
  • Tsutomu Arie
  • Ken KomatsuEmail author


Plantago asiatica mosaic virus (PlAMV) is a member of the genus Potexvirus in the family Alphaflexiviridae and has been isolated from a variety of host plants. In particular, PlAMV isolates from ornamental lilies (Lilium spp.) cause necrotic symptoms in these plants, which significantly reduces their commercial value. However, it has not been clear whether PlAMV isolates from other host plants differ in their infectivity and/or pathogenicity to ornamental lilies, and whether growth conditions affect infectivity and pathogenicity. In this study, we inoculated an edible lily species (Lilium leichtlinii) and seven varieties of ornamental lilies with two PlAMV isolates, an isolate from ornamental lily (PlAMV-OL) and an isolate from edible lily (PlAMV-Li1). We found that PlAMV-OL showed higher infection rates and exhibited necrotic symptoms more frequently in lilies than PlAMV-Li1. Moreover, we observed higher infection rates of PlAMV-OL in open field than in greenhouse, and higher rates of necrotic symptoms in autumn test than in spring test, suggesting that growth conditions and season affect infectivity and pathogenicity of PlAMV in lilies. Our study would provide important information for estimating the risk of necrotic disease caused by PlAMV, as well as for cultivation management preventing the occurrence of the disease.


Plantago asiatica mosaic virus Ornamental lily Necrosis Infectivity Pathogenicity Environmental effects 



We thank YAMAKI NOEN CORPOLATION for providing lily bulbs and cultivation site. This work was supported by Overseas travel assistance program executed by TUAT president discretionary fund.

Compliance with ethical standards

Informed consent

The paper has not been submitted elsewhere for publication, in whole or in part.

Conflict of interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Ethical approval

This paper does not contain studies on human or animal participants.

Supplementary material

10658_2018_1594_MOESM1_ESM.docx (192 kb)
Figure. S1 Detection of LMoV from lily bulbs used for inoculation test. Amplicons by RT-PCR were analyzed in a 1% agarose gel. M: 1kbp DNA Ladder (New England BioLabs, USA), lane 1: A sample from a LMoV-infected lily plant (positive control), lane 2 to 13: 12 samples from L. ‘Sheila’ bulbs. Arrow indicates amplicons from LMoV (767 bp). (DOCX 192 kb)
10658_2018_1594_MOESM2_ESM.docx (1.8 mb)
Figure. S2 Necrotic symptoms observed in leaves (a) and stems (b) of edible lily inoculated with PlAMV-OL about 2 months post inoculation. (DOCX 1845 kb)
10658_2018_1594_MOESM3_ESM.docx (607 kb)
Figure. S3 Local lesions observed in four leaves of Chenopodium quinoa inoculated with PlAMV-Li1 (a) and PlAMV-OL (b). (DOCX 606 kb)
10658_2018_1594_MOESM4_ESM.docx (19 kb)
Supplementary Table 1 (DOCX 18 kb)


  1. Chen, K. C., Chiang, C. H., Raja, J. A. J., Liu, F. L., Tai, C. H., & Yeh, S. D. (2008). A single amino acid of NIaPro of Papaya ringspot virus determines host specificity for infection of papaya. Molecular Plant-Microbe Interactions, 21(8), 1046–1057.CrossRefGoogle Scholar
  2. Dahal, G., Hughes, D. A., Thottappilly, G., & Lockhart, B. E. L. (1998). Effect of temperature on symptom expression and reliability of Banana streak badnavirus detection in naturally infected plantain and banana (Musa spp.). Plant Disease, 82(1), 16–21.CrossRefGoogle Scholar
  3. EPPO Reporting Service (2011). New pest records in EPPO member countries: Plantago asiatica mosaic virus (Potexvirus, PlAMV) found on Lilium spp. in the Netherlands. EPPO Reporting Service 2011/082. Accessed 09 Aug 2014.
  4. Hammond, J., Bampi, D., & Reinsel, M. D. (2015). First report of Plantago asiatica mosaic virus in imported asiatic and oriental lilies (Lilium hybrids) in the United States. Plant Disease, 99(2), 292.CrossRefGoogle Scholar
  5. Hughes, P. L., Harper, F., Zimmerman, M. T., & Scott, S. W. (2005). Nandina mosaic virus is an isolate of Plantago asiatica mosaic virus. European Journal of Plant Pathology, 113, 309–313.CrossRefGoogle Scholar
  6. Kido, K., Tanaka, C., Mochizuki, T., Kubota, K., Ohki, T., Ohnishi, J., Knight, L. M., & Tsuda, S. (2008). High temperatures activate local viral multiplication and cell-to-cell movement of Melon necrotic spot virus but restrict expression of systemic symptoms. Phytopathology, 98(2), 181–186.CrossRefGoogle Scholar
  7. Komatsu, K., Yamaji, Y., Ozeki, J., Hashimoto, M., Kagiwada, S., Takahashi, S., & Namba, S. (2008). Nucleotide sequence analysis of seven Japanese isolates of Plantago asiatica mosaic virus (PlAMV): A unique potexvirus with significantly high genomic and biological variability within the species. Archives of Virology, 153, 193–198.CrossRefGoogle Scholar
  8. Komatsu, K., Yamashita, K., Sugawara, K., Verbeek, M., Fujita, N., Hanada, K., Uehara-Ichiki, T., & Fuji, S. (2017). Complete genome sequences of two highly divergent Japanese isolates of Plantago asiatica mosaic virus. Archives of Virology, 162, 581–584.CrossRefGoogle Scholar
  9. Kostin, V. D., & Volkov, Y. G. (1976). Some properties of the virus affecting Plantago asiatica L. Virusnye Bolezni Rastenji Dalnego Vostoka (in Russian), 25, 205–210.Google Scholar
  10. Ma, L., Huang, X., Yu, R., Jing, X. L., Xu, J., Wu, C. A., Zhu, C. X., & Liu, H. M. (2016). Elevated ambient temperature differentially affects virus resistance in two tobacco species. Phytopathology, 106(1), 94–100.CrossRefGoogle Scholar
  11. Martelli, G. P., Adams, M. J., Kreuze, J. F., & Dolja, V. V. (2007). Family Flexiviridae: A case study in virion and genome plasticity. Annual Review of Phytopathology, 45, 73–100.CrossRefGoogle Scholar
  12. Ozeki, J., Takahashi, S., Komatsu, K., Kagiwada, S., Yamashita, K., Mori, T., Hirata, H., Yamaji, Y., Ugaki, M., & Namba, S. (2006). A single amino acid in the RNA-dependent RNA polymerase of Plantago asiatica mosaic virus contributes to systemic necrosis. Archives of Virology, 151(10), 2067–2075.CrossRefGoogle Scholar
  13. Pájtli, É., Eke, S., & Palkovics, L. (2015). First report of the Plantago asiatica mosaic virus (PlAMV) incidence on Lilium sp. in Hungary. Plant Disease, 99(9), 1288.CrossRefGoogle Scholar
  14. Pappi, P. G., Chaintoutis, S. C., Dovas, C. I., Efthimiou, K. E., & Katis, N. I. (2014). Development of one-tube real-time qRT-PCR and evaluation of RNA extraction methods for the detection of eggplant mottled dwarf virus in different species. Journal of Virological Methods, 212, 59–65.CrossRefGoogle Scholar
  15. Parrella, G., Greco, B., Pasqualini, A., & Nappo, A. G. (2015). Plantago asiatica mosaic virus found in protected crops of lily hybrids in southern Italy. Plant Disease, 99(9), 1289.CrossRefGoogle Scholar
  16. Saitoh, H., Saiga, T., Ohki, S. T., & Osaki, T. (1998). Systemic resistance in Cucumis figarei to some strains of cucumber mosaic virus is breakable at high temperature. Annals of the Phytopathological Society of Japan, 64, 194–197.CrossRefGoogle Scholar
  17. Sasaki, J. (2008). Virus diseases of edible lily and disease control by virus free plants. Plant Protection, 62, 405–409 (in Japanese).Google Scholar
  18. Sato, H., Hagiwara, K., Nakamura, S., Morikawa, T., Honda, Y., & Omura, T. (2002). A comparison of sensitive and specific methods for the detection of lily mottle virus in lily plants. Journal of Phytopathology, 150, 20–24.CrossRefGoogle Scholar
  19. Solovyev, A. G., Novikov, V. K., Merits, A., Savenkov, E. I., Zelentina, D. A., Tyulkina, L. G., & Morozov, S. Y. (1994). Genome characterization and taxonomy of Plantago asiatica mosaic potexvirus. Journal of General Virology, 75, 259–267.CrossRefGoogle Scholar
  20. Szittya, G., Silhavy, D., Molnár, A., Havelda, Z., Lovas, Á., Lakatos, L., Bánfalvi, Z., & Burgyán, J. (2003). Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation. The EMBO Journal, 22, 633–640.CrossRefGoogle Scholar
  21. iBulb. (2016). Lilies as cut flowers and as pot plants. Guidelines for producing lilies as cut flowers and pot plants, section 9.8.4. Hillegom, the Netherlands: iBulb, Accessed 22 June 2018.
  22. Vidal, A. K., Camps, R., & Besoain, X. (2016). First report of necrotic streaking of asiatic lilies caused by Plantago asiatica mosaic virus in Chile. Plant Disease, 100, 1799.CrossRefGoogle Scholar
  23. Vignuzzi, M., Stone, J. K., Arnold, J. J., Cameron, C. E., & Andino, R. (2006). Quasispecies diversity determines pathogenesis through cooperative interactions in a viral population. Nature, 439(19), 344–348.CrossRefGoogle Scholar
  24. Xu, L. F., Ming, J., & Yuan, S. X. (2017). First report of Plantago asiatica mosaic virus in lily hybrids in China. Plant Disease, 101(1), 263.CrossRefGoogle Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  1. 1.Laboratory of Plant Pathology, Graduate School of AgricultureTokyo University of Agriculture and Technology (TUAT)FuchuJapan
  2. 2.Wageningen University and ResearchWageningenThe Netherlands
  3. 3.Wageningen University and ResearchLisseThe Netherlands
  4. 4.Institute of Global Innovation ResearchTokyo University of Agriculture and TechnologyFuchuJapan

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