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European Journal of Plant Pathology

, Volume 153, Issue 3, pp 759–770 | Cite as

Characterization and management of watermelon bud necrosis virus infecting watermelon in India

  • R. Priyanka
  • K. NagendranEmail author
  • R. Aravintharaj
  • C. G. Balaji
  • S. Mohankumar
  • P. Renukadevi
  • G. Karthikeyan
Article
  • 95 Downloads

Abstract

Bud necrosis disease is an emerging threat for watermelon cultivation in India. It is characterized by necrotic spots and patches on leaves, bud and fruits with characteristic chlorotic ring spots as documented in Coimbatore and Villupuram districts of Tamil Nadu, India. The causative agent of this disease was identified as watermelon bud necrosis virus (WBNV) by reverse-transcription polymerase chain reaction (RT-PCR) followed by sequence analysis of amplified fragment using Orthotospovirus universal degenerate primer pair. Upon mechanical inoculation, the virus produced circular chlorotic local lesions on leaves of cowpea and Chenopodium amaranticolor, necrotic local lesions on Trianthema portulacastrum and bottle gourd, systemic vein clearing on Luffa aegyptiaca, whereas both local and systemic symptoms were observed on Nicotiana tabacum and watermelon. The complete nucleotide sequences of the coat protein (NP), movement protein (MP), replicase (RdRp) and NSs protein genes from WBNV genome of Coimbatore isolate (TN KTP WM1) had a maximum identity of 97% with the genome of the WBNV-JT strain from Southern India. WBNV infection causes significant reduction in nutritional parameters such as carbohydrate, protein, crude fibre, calorific value, total sugar, reducing sugar and vitamin A in infected fruits when compared to the healthy ones. For the management of WBNV in watermelon, an Integrated Pest Management (IPM) module has been devised which reduced the thrips population and WBNV incidence to a great extent besides increasing the fruit yield.

Keywords

Tospovirus Virus management Thrips Cucurbits Bud necrosis Watermelon virus 

Notes

Acknowledgements

We are grateful to Dr. R.A. Naidu (WSU, USA) for his valuable guidance and suggestions in conducting this study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Current research does not involved human participants or animals.

Supplementary material

10658_2018_1589_MOESM1_ESM.docx (31 kb)
ESM 1 (DOCX 30 kb)
10658_2018_1589_MOESM2_ESM.pptx (208 kb)
ESM 2 (PPTX 207 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • R. Priyanka
    • 1
  • K. Nagendran
    • 1
    • 2
    Email author
  • R. Aravintharaj
    • 1
    • 3
  • C. G. Balaji
    • 1
  • S. Mohankumar
    • 4
  • P. Renukadevi
    • 1
  • G. Karthikeyan
    • 1
  1. 1.Department of Plant PathologyTamil Nadu Agricultural UniversityCoimbatoreIndia
  2. 2.Division of Crop ProtectionICAR – Indian Institute of Vegetable ResearchVaranasiIndia
  3. 3.Division of BiotechnologyICAR-Indian Institute of Horticultural ResearchBengaluruIndia
  4. 4.Department of Plant Molecular Biology and BiotechnologyTamil Nadu Agricultural UniversityCoimbatoreIndia

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