Genetic diversity and pathogenicity dynamics of Magnaporthe oryzae in the Wuling Mountain area of China
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Rice blast, caused by Magnaporthe oryzae (M. oryzae), is one of the most destructive diseases in cultivated rice. The Wuling Mountain Area is marked as the key area for the national prevention and control of rice blast disease in China and is the ideal area for M. oryzae research according to its unique natural condition. In this study, a simple, low-cost and effective marker system including RAPD, REMAP, rep-PCR and Avr genes was developed for genetic diversity analysis of 108 M. oryzae isolates collected from the Wuling Mountain Area during 2012–2014. The results showed significant changes in the genetic structures of M. oryzae populations over the 3 years. However, there was no remarkably simple relationship between the genetic lineages and pathotypes (physiological races) of M. oryzae. The main mechanisms of M. oryzae to overcome host genes in the natural conditions of the Wuling Mountain Area might be point mutations or small deletions of gene sequences.
KeywordsGenetic diversity Pathogenicity dynamics Magnaporthe oryzae
We gratefully acknowledge Dr. Xiaoyuan Zhu (the Plant Protection Research Institute of the Guangdong Academy of Agricultural Sciences, China) for the help of disease evaluation.
We are grateful for grants from the Science Funds for the Creative Research Groups of Hubei Province, China (No. 2015CFA015) and the Fundamental Research Funds for the Central Universities, South-Central University for Nationalities, China (No. CZP17029 & CZP17089).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Hammer, Ø., Harper, D. A.T., & Ryan, P. D. (2001). PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica, 4, 1–9.Google Scholar
- Khush, G. S., & Jena, K. K. (2009). Current status and future prospects for research on blast resistance in rice (Oryza sativa L.). In G.-L. Wang & B. Valent (Eds.), Advances in genetics, genomics and control of Rice blast disease (pp. 1–10). Dordrecht: Springer Netherlands.Google Scholar
- Kiyosawa, S. (1984). Establishment of differential varieties for pathogenicity test of rice blast fungus. Rice Genetics Newsletter, 1, 95–97.Google Scholar
- Kumar, J., Nelson, R. J., & Zeigler, R. S. (1999). Population structure and dynamics of Magnaporthe grisea in the Indian Himalayas. Genetics, 152, 971–984.Google Scholar
- Li, M., Wang, C. T., Zhou, J., & Xu, X. (2014). Genetic diversity of Magnaporthe oryzae in western Hubei Province in 2011. Journal of Anhui Agricultural Sciences, 42, 4249–4251.Google Scholar
- Lu, M. H., Liu, W. C., Zhu, F., Zhang, Q. D., & Xia, F. (2015). Investigation of the causes and countermeasures of rice blast severe outbreak in 2014 (in Chinese). China Plant Protection, 35, 35–39.Google Scholar
- Pagliaccia, D., Urak, R. Z., Wong, F., Douhan, L. A. I., Greer, C. A., Vidalakis, G., & Douhan, G. W. (2018). Genetic structure of the rice blast pathogen (Magnaporthe oryzae) over a decade in North Central California rice fields. Microbial Ecology, 75, 310–317Google Scholar
- Selisana, S. M., Yanoria, M. J., Quime, B., Chaipanya, C., Lu, G., Opulencia, R., & Zhou, B. (2017). Avirulence (AVR) gene-based diagnosis complements existing pathogen surveillance tools for effective deployment of resistance (R) genes against rice blast disease. Phytopathology, 107, 711–720.CrossRefGoogle Scholar
- Tian, K., Yang, W., Li, M., Xu, X., Liu, X. Q., Liu, X. Q., & Wang, C. T. (2017). Pathogenicity changes of Magnaporthe oryzae in South-Western Hubei Province during 2012-2014 (in Chinese with English abstract). Journal of Huazhong Agricultural University, 36, 10–14.Google Scholar
- Wu, J., Kou, Y. J., Bao, J. D., Li, Y., Tang, M. Z., Zhu, X. L., Ponaya, A., Xiao, G., Li, J. B., Li, C. Y., Song, M. Y., Cumagun, C. J. R., Deng, Q. Y., Lu, G. D., Jeon, J. S., Naqvi, N. I., & Zhou, B. (2015). Comparative genomics identifies the Magnaporthe oryzae avirulence effector AvrPi9 that triggers Pi9-mediated blast resistance in rice. New Phytologist, 206, 1463–1475.CrossRefGoogle Scholar
- Yang, X. L., Shi, S. S., Zhang, S., Liang, L. V., & Yu, D. Z. (2016). Population pathotype of Magnaporthe oryzae in rice blast epidemic areas of Hubei Province. Hubei Agricultural Sciences, 55(4169–4171), 4175.Google Scholar