European Journal of Plant Pathology

, Volume 153, Issue 2, pp 545–555 | Cite as

Race and virulence of asexual and sexual populations of Puccinia graminis f. sp. tritici in China from 2009 to 2015

  • Yuanyin CaoEmail author
  • Binbin Si
  • Guiqing Zhu
  • Xiaofeng Xu
  • Weihua Li
  • Si Chen
  • Jie Zhao
  • Tianya LiEmail author


Stem rust of wheat caused by Puccinia graminis f. sp. tritici (Pgt) has been under control in China for decades but new races including the Ug99 race group are an ongoing threat. Pgt race surveillance is an essential component for disease prevention strategies. Three hundred fourteen isolates (284 asexual and 30 sexual) obtained from 2009 to 2015 in China were tested on a panel of differential lines and categorized into five race groups (21C3, 34C0, 34C1, 34C3, and 34C6) and 29 races. Race group 21C3 was most frequent (64.0%) and race 21C3CTHTM was the most prevalent (16.6%). Race group 34C6 and 15 races were detected for the first time, accounting for 2.5 and 27.4% of the isolates, respectively. The most widely used stem rust resistance gene (Sr31) is effective against all isolates, however other commonly used resistance genes (Sr5, and Sr17) are only effective to some of the isolates. Novel Pgt races with virulence to both Sr5 and Sr11 were detected. Isolates from the sexual population were strikingly different from those of the asexual population, having higher frequency of new race group, new races and new virulence combination (Sr5 + Sr11). In addition, the higher variation in sexual Pgt population observed in the region growing wild barberries suggests that these sites might be responsible for the occurrence of new races in major wheat growing areas in China.


Physiological races Sexual population Puccinia graminis f. sp. tritici Berberis Sr genes 



This study was supported by the National Key Basic Research Program of China (2013CB127701), the Special Fund for Agro-Scientific Research in the Public Interest (201303016) and the National Natural Science Foundation of China (31171829). The great deal of effort was made by our colleagues to get asexual samples in different locations. We deeply appreciate their assistance.

Compliance with ethical standards

Conflicts of interest

This manuscript has not been published or presented elsewhere in part or in entirety and is not under consideration by another journal. All authors have read and understood your journal’s Ethical Standards and Informed consent, and we believe that neither the manuscript nor the study violates any of these. There are no conflicts of interest to declare, and don’t involve Human Participants and Animals. All authors agree to submit article to European Journal of Plant Pathology.

Supplementary material

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ESM 1 (XLSX 17 kb)


  1. Abebe, T., Woldeab, G., & Dawit, W. (2012). Distribution and physiologic races of wheat stem rust in Tigray, Ethiopia. Journal of Plant Pathology and Microbiol, 3, 142. Scholar
  2. Bushnell, W. R., & Roelfs, A. P. (1984). The Cereal Rusts, Volume I. Origins, Specificity, Structure and Physiology (p. 546). Orlando, San Diego, San Francisco, New York, London, Toronto, Montreal, Sydney, Tokyo, and Sao Paulo: Academic Press, Inc.Google Scholar
  3. Cao, Y. Y., Yao, P., Zhu, G. Q., & Wu, Y. S. (1994). A preliminary analysis of probable genes for stem rust resistance and resistance stability of 41 wheat cultivars in China. Journal of Shenyang Agricultural University, 25, 392–397.Google Scholar
  4. Cao, Y. Y., Yao, P., Liu, W. Z., & Wu, Y. S. (1996a). Pathogenic spectrum analysis of 21C3CTR of Puccinia graminis f. sp. tritici in China. Journal of Shenyang Agricultural University, 27, 26–30.Google Scholar
  5. Cao, Y. Y., Yao, P., Zhu, G. Q., Liu, W. Z., & Wu, Y. S. (1996b). Application of computer to inferring stem rust resistance genes commercial wheat cultivars. Scientia Agricultura Sinica, 29, 89–91.Google Scholar
  6. Cao, Y. Y., Yao, P., Wu, Y. S., Bi, Y. Q., & Yang, J. X. (2001). Discovery and verification of an important inoculum source for Puccinia graminis f. sp. tritici in China. Acta Phytophylacica Sinica (Journal of Plant Protection), 28, 294–298.Google Scholar
  7. Cao, Y. Y., Han, J. D., Zhu, G. Q., & Zhang, L. (2007). Ug99, a new virulent race of Puccinia graminis f. sp. tritici, and its effect on China. Plant Protection, 33, 86–89.Google Scholar
  8. CIMMYT (2007). Dangerous wheat disease jumps Red Sea. Devastating fungal pathogen spreads from Eastern Africa to Yemen, following path scientists predicted. pub_releases/2007–01/imaw-dwd011607.php.
  9. CIMMYT EXPERT PANEL (2005). Sounding the alarm on global stem rust.
  10. Han, J. D. (2009). Resistant gene control and related mechanism to the invasion of race Ug99 of Puccinia graminis f. sp. tritici. [master’s thesis]. [Shenyang]: Shenyang Agricultural University.Google Scholar
  11. Han, J. D., Cao, Y. Y., & Sun, Z. G. (2010). Race dynamics of Puccinia graminis f. Sp. tritici in China and the virulence of CIMMYT wheat germplasms resistant to Ug99. Journal of Triticeae Crops, 30, 163–166.Google Scholar
  12. Jin, Y. (2011). Role of Berberis spp. as alternate hosts in generating new races of Puccinia graminis and P. striiformis. Euphytica, 179, 105–108. Scholar
  13. Jin, Y., Szabo, L. J., Rouse, M., Fetch, T., Pretorius, Z. A., Wanyera, R., & Njau, P. (2009). Detection of virulence to resistance gene Sr36 within the TTKS race lineage of Puccinia graminis f. sp. tritici. Plant Disease, 92, 923–926. Scholar
  14. Jin, Y., Szabo, L. J., & Carson, M. (2010). Century-old mystery of Puccinia striiformis life history solved with the identification of Berberis as an alternate host. Phytopathology, 100, 432–435. Scholar
  15. Li, Z. Q., & Zeng, S. M. (2002). Wheat rust in China (1st ed.p. 379). Beijing: China Agricultural Press.Google Scholar
  16. Li, T. Y., Cao, Y. Y., Wu, X. X., Xu, X. F., & Wang, W. L. (2016a). Seedling resistance to stem rust and molecular marker analysis of resistance genes in wheat cultivars of Yunnan, China. PLoS ONE, 11(10), e0165640. Scholar
  17. Li, T. Y., Wu, X. X., Xu, X. F., Wang, W. L., & Cao, Y. Y. (2016b). Postulation of seedling stem rust resistance genes of Yunnan wheat cultivars in China. Plant Protection Science, 4, 242–249. Scholar
  18. Liu, A. F., Li, H. S., Song, J. M., Chen, D. G., Zhao, Z. D., & Liu, J. J. (2009). Resistance analysis of stem rust resistance genes to new virulent pathogen Ug99 in common wheat. Shandong Agricultural Science, 6, 61–63.Google Scholar
  19. Mago, R., Bariana, H. S., Dundas, I. S., Spielmeyer, W., Lawrence, G. J., Pryor, A. J., & Ellis, J. (2005). Development of PCR markers for the selection of wheat stem rust resistance genes Sr24 and Sr26 in diverse wheat germplasm. Theoretical Applied of Genetics, 111, 496–504. Scholar
  20. Nazari, K., Mafi, M., Yahyaoui, A., Singh, R. P., & Park, R. F. (2009). Detection of wheat stem rust race (Puccinia graminis f. Sp. tritici) TTKSK (Ug99) in Iran. Plant Disease, 93, 317. Scholar
  21. Pardey, P. G., Beddow, J. M., Kriticos, D. J., Hurley, T. M., Park, R. F., Duveiller, E., Sutherst, R. W., Burdon, J. J., & Hodson, D. (2013). Right-sizing stem-rust research. Science, 340, 147–148. Scholar
  22. Roelfs, A. P., & Martens, J. W. (1988). An international system of nomenclature for Puccinia graminis f. sp. tritici. Phytopathology, 78, 526–533.CrossRefGoogle Scholar
  23. Sajid, A., Syed, J. A., Hidayatur, R., Muhammad, S. S., Muhammad, I., & Muhammad, S. (2009). Variability in wheat yield under yellow rust pressure in Pakistan. Turkey Journal of Agricultural Forest, 33, 537–546. Scholar
  24. Singh, R. P., Hodson, D. P., Huerta-Espino, J., Jin, Y., Bhavani, S., Njau, P., Herrera-Foessel, S., Singh, P. K., Singh, S., & Govindan, V. (2011). The emergence of Ug99 races of the stem rust fungus is a threat to world wheat production. Annual Review of Phytopathology, 49, 465–481. Scholar
  25. Wanyera, R., & Kinyua, M. G. (2006). The spread of stem rust caused by Puccinia graminis f. Sp. tritici, with virulence on Sr31 in wheat in eastern Africa. Plant Disease, 90, 113. Scholar
  26. USDA, Foreign Agricultural Service (2014). World Agricultural Production. October 2014.
  27. Wu, Y. S., & Huang, Z. T. (1987). Twenty years’ racial identification and fluctuation analysis of Puccinia graminis f. sp. tritici in China. Journal of Shenyang Agricultural University, 18, 105–138.Google Scholar
  28. Yao, P., Cao, Y. Y., Liu, W. Z., & Wu, Y. S. (1995). Race dynamics of Puccinia graminis f. Sp. tritici in China in 1993. Journal of Plant Protection, 22, 303–308.Google Scholar
  29. Ying, J. S., & Chen, D. Z. (2001). Flora reipublicae popularis sinicae, tomus 29-Lardizabalaceae and Berberidaceae. Sciences Press, 50–248.Google Scholar
  30. Zhao, J., Wang, L., Wang, Z., Chen, X. M., Zhang, H. C., Yao, J. N., Zhan, G., Chen, W., Huang, L., & Kang, Z. (2013). Identification of eighteen Berberis species as alternate hosts of Puccinia striiformis f. Sp. tritici and virulence variation in the pathogen isolates from natural infection of barberry plants in China. Phytopathology, 103, 927–934.CrossRefPubMedGoogle Scholar
  31. Zhao, J., Zhao, S. L., Chen, X. M., Wang, Z. Y., Wang, Z., & Yao, J. N. (2015). Determination of the role of Berberis spp. in wheat stem rust in China. Plant Disease, 99, 1113–1117.CrossRefPubMedGoogle Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  1. 1.College of Plant ProtectionShenyang Agricultural UniversityShenyangChina
  2. 2.College of Plant ProtectionNorthwest A &F UniversityYanglingChina

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