A comparative proteomic analysis of the PVY-induced hypersensitive response in leaves of potato (Solanum tuberosum L.) plants that differ in Ny-1 gene dosage
- 211 Downloads
The Ny-1 gene confers hypersensitive response (HR) to Potato virus Y (PVY) in potato cultivars (Solanum tuberosum L.). Tetraploid potato breeding clone PB07–37 possessing the allele Ny-1 in a duplex state was developed. After PVY infection, the size of necrotic lesions in leaves of PB07–37 was reduced by approximately 68% in relation to cultivar Rywal plants displaying Ny-1 in a simplex dosage. Two-dimensional gel electrophoresis (2-DE) was applied for screening PVY-induced proteins in leaves of PB07–37. Compared with non-infected control plants, 60 reproducible PVY-induced proteins were detected using LC-MS/MS analysis, of which 41 were involved in qualitative changes and 19 were differently expressed in inoculated leaves by at least 1.5-fold. Proteins involved in the category of photosynthesis and primary metabolism were the most abundant. The results from PB07–37 (Ny-1 duplex) were compared with data from Rywal (Ny-1 simplex). The protein profiles in the Ny-1 simplex and Ny-1 duplex plants are genotype specific. Only eight proteins were identified in both genotypes. Five of them: ATP synthase CF1 alpha chain, chloroplastic; ATP synthase CF1 beta subunit, chloroplastic; ATP synthase subunit beta, mitochondrial-like; linoleate 13S-lipoxygenase 2–1, chloroplastic; and mitochondrial monodehydroascorbate reductase 5 are known to be involved in the defense response in plants. In our study, however, protein abundance did not correspond to the Ny-1 resistance gene dosage.
KeywordsHypersensitive response Leaf proteomes Ny-1 resistance gene dosage Potato PVY
The research was supported by The National Science Centre in Poland, grant UMO-2014/13/B/NZ9/02468.
Compliance with ethical standards
Conflict of interest
No conflict of interest.
Human and/or animals rights
- Bengtsson, T., Weighill, D., Proux-Wéra, E., Levander, F., Resjö, S., Burra, D. D., Moushib, L. I., Hedley, P. E., Liljeroth, E., Jacobson, D., Alexandersson, E., & Andreasson, E. (2014). Proteomics and transcriptomics of the BABA-induced resistance response in potato using a novel functional annotation approach. BMC Genomics, 15, 315.CrossRefPubMedPubMedCentralGoogle Scholar
- Collmer, C. W., Marston, M. F., Taylor, J. C., & Jahn, M. (2000). The I gene of bean: A dosage-dependent allele conferring extreme resistance, hypersensitive resistance, or spreading vascular necrosis in response to the potyvirus Bean common mosaic virus. Molecular Plant-Microbe Interactions, 13, 1266–1270.CrossRefPubMedGoogle Scholar
- Croft, K. P. C., Voisey, C. R., & Slusarenko, A. J. (1990). Mechanism of hypersensitive cell collapse: Correlation of increased lipoxygenase activity with membrane damage in leaves of Phaseolus vulgaris (L.) inoculated with an avirulant race of Pseudomonas syringae pv. phaseolicola. Physiological and Molecular Plant Pathology, 36, 49–62.CrossRefGoogle Scholar
- Flis, B., Hennig, J., Strzelczyk-Żyta, D., Gebhardt, C., & Marczewski, W. (2005). The Ry-f sto gene from Solanum stoloniferum for extreme resistant to Potato virus Y maps to potato chromosome XII and is diagnosed by PCR marker GP122718 in PVY resistant potato cultivars. Molecular Breeding, 15, 95–101.CrossRefGoogle Scholar
- Hatsugai, N., Koldenkova, V. P., Imamura, H., Noji, H., & Nagai, T. (2012). Changes in cytosolic ATP levels and intracellular morphology during bacteria-induced hypersensitive cell death as revealed by real-time fluorescence microscopy imaging. Plant and Cell Physiology, 53, 1768–1775.CrossRefPubMedGoogle Scholar
- Rance, I., Fournier, J., & Esquerré-Tugayé, M.-T. (1998). The incompatible interaction between Phytophthora parasitica var. nicotianae race 0 and tobacco is suppressed in transgenic plants expressing antisense lipoxygenase sequences. Proceedings of the National Academy of Sciences of USA, 95, 6554–6559.CrossRefGoogle Scholar
- Rühle, T., & Leister, D. (2015). Assembly of F1F0-ATP synthases Biochimica et Biophysica. Acta, 1847, 849–860.Google Scholar
- Sagredo, B. D., Mathias, M. R., Barrientos, C. P., Acuña, I. B., Kalazich, J. B., & Santos, J. R. (2009). Evaluation of a SCAR RYSC3 marker of the Ry adg gene to select resistant genotypes to Potato virus Y (PVY) in the INIA potato breeding program. Chilean Journal of Agricultural Research, 69, 305–315.CrossRefGoogle Scholar
- Szajko, K., Chrzanowska, M., Witek, K., Strzelczyk-Żyta, D., Zagórska, H., Gebhardt, C., Hennig, J., & Marczewski, W. (2008). The novel gene Ny-1 on potato chromosome IX confers hypersensitive resistance to Potato virus Y and is an alternative to Ry genes in potato breeding for PVY resistance. Theoretical and Applied Genetics, 116, 297–303.CrossRefPubMedGoogle Scholar
- Szarzynska, B., Sobkowiak, L., Pant, B. D., Balazadeh, S., Scheible, W. R., Mueller-Roeber, B., Jarmolowski, A., & Szweykowska-Kulinska, Z. (2009). Gene structures and processing of Arabidopsis thaliana HYL1-dependent primiRNAs. Nucleic Acids Research, 37, 3083–3093.CrossRefPubMedPubMedCentralGoogle Scholar