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European Journal of Plant Pathology

, Volume 153, Issue 2, pp 341–357 | Cite as

Multiple Halophytophthora spp. and Phytophthora spp. including P. gemini, P. inundata and P. chesapeakensis sp. nov. isolated from the seagrass Zostera marina in the Northern hemisphere

  • Willem A. Man in ’t VeldEmail author
  • Karin C. H. M. Rosendahl
  • Patricia C. J. van Rijswick
  • Johan P. Meffert
  • Edu Boer
  • Marcel Westenberg
  • Tjisse van der Heide
  • Laura L. Govers
Article
  • 85 Downloads

Abstract

A plethora of oomycetes was isolated mostly from Zostera marina but also from other halophilic plants originating from several locations including the Wadden Sea, Limfjord (Denmark), the Dutch Delta area (the Netherlands), Thau lagoon (France), Lindholmen (Sweden) and Chesapeake Bay (Virginia, U.S.). Based on ITS sequences, seven different groups could be distinguished. The largest group was assigned to Phytophthora gemini (Germany, Sweden, the Netherlands, U.S.). The CoxI sequences of all P. gemini strains were identical indicating that P. gemini is probably an invasive species in the Wadden Sea. A second group was identified as P. inundata (the Netherlands, Denmark), that was also isolated from the halophilic plants Aster tripolium and Salicornia europaea. Four strains, originating from Chesapeake Bay clustered in a monophyletic clade with high bootstrap support at the ITS as well as the CoxI loci. They are phylogenetically closely related to P. gemini and are considered to represent a new species described here as Phytophthora chesapeakensis sp. nov. In addition, Salisapilea sapeloensis was isolated from Zostera noltii. Eleven other strains belonging to three unidentified taxa, originating from the Wadden Sea, the Dutch Delta area and Thau lagoon, clustered each in a monophyletic clade with high bootstrap support at the ITS locus, including Halophytophthora vesicula, the type species of the genus Halophytophthora. Hence, these strains were considered to belong to the Halophytophthora sensu stricto group and probably represent three new Halophytophthora species, informally designated here as Halophytophthora sp-1, Halophytophthora sp-3 and Halophytophthora sp-4 sensu Nigrelli and Thines. Halophytophthora sp-2 was not detected in this study. In addition, P. gemini and Halophytophthora sp-3 were obtained by baiting from locations in the Wadden Sea and Halophytophthora sp-1 was obtained by baiting from the Delta area.

Keywords

Cytochrome oxidaseI Invasive species ITS Marine oomycete species New species Phylogeny 

Notes

Acknowledgements

The authors thank Mike Coffey (US) for sequencing Halophytophthora sp-4 and Robert Orth (US), Eduardo Infantes, Per-Olav Moksnes, Louise Eriander (Sweden), Birgit Olesen, Flemming Gertz (Denmark), Matthijs van de Geest (France), Dick de Jong (The Netherlands) for their generous gift of Z. marina seeds and the Fieldwork Company (Jannes Heusinkveld, Remco de Nooij) for installation and collection of baits from the Dutch Wadden Sea area.

Compliance with ethical standards

Conflict of interest

The authors declare they have no conflict of interest.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Willem A. Man in ’t Veld
    • 1
    Email author
  • Karin C. H. M. Rosendahl
    • 1
  • Patricia C. J. van Rijswick
    • 1
  • Johan P. Meffert
    • 1
  • Edu Boer
    • 1
  • Marcel Westenberg
    • 1
  • Tjisse van der Heide
    • 2
  • Laura L. Govers
    • 2
    • 3
  1. 1.Dutch National Plant Protection OrganizationNational Reference CentreWageningenNetherlands
  2. 2.Department of Aquatic Ecology and Environmental Biology Institute for Water and Wetland Research (IWWR)Radboud UniversityNijmegenNetherlands
  3. 3.Conservation Ecology Group, Groningen Institute for Evolutionaire Life Sciences (GELIFES)University of GroningenGroningenNetherlands

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