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European Journal of Plant Pathology

, Volume 153, Issue 1, pp 153–166 | Cite as

Virulence and molecular genetic diversity of the Puccinia triticina population in Hebei Province of China in 2008 and 2010

  • L. Y. Zhang
  • Q. F. Meng
  • H. F. YanEmail author
  • D. Q. Liu
Article

Abstract

Wheat leaf rust, caused by Puccinia triticina, is a commonly occurring disease in wheat growing areas of the world. Research on the virulence and genetic diversity of P. triticina will be helpful for a better understanding of the evolution tendencies and subsequently for controlling this disease. In this study, 59 isolates of P. triticina collected from Hebei Province of China in 2008 and 2010 were analyzed for virulence diversity based on 37 near-isogenic Thatcher wheat lines at seedling stage, and genetic diversity using 21 pairs of EST-SSR primers. The predominant virulence phenotypes were PHTT, THTT, and THJT in 2008, while THTS, THSS, THTT, and THST were most common in Hebei province in 2010. Clustering analysis based on the virulence and EST-SSR data revealed a high diversity of P. triticina, and differences between the populations of 2008 and 2010. There was no significant correlation between the molecular and virulence data. Genetic parameters analysis also showed high diversity of the P. triticina population in Hebei province. Nei’s gene diversity index (H) and Shannon’s information index (I) showed that the genetic diversity of population 2008 was higher than 2010. The AMOVA analysis suggested that genetic variation mainly from intra-population (61.11%).

Keywords

Wheat leaf rust EST-SSR Virulence Genetic diversity 

Notes

Acknowledgements

The authors thank Ian Dundas (Adelaide University, Australia) for critical reading of the manuscript. This research was supported by National Key Basic Research Program of China (2013CB127700), and Special Fund for Natural Science Fund Project of Hebei Province (C2015204105).

Funding

This study was funded by 2013CB127700 and C2015204105.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Human and animal rights

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Ethical statement

The manuscript has not been submitted to more than one journal for simultaneous consideration, and has not been published previously. This is a single study not being split up into several parts and not being submitted to various journals or to one journal over time. No data have been fabricated or manipulated to support our conclusions. We don’t plagiarism others’ data, text, or theories. Consent to submit has been received explicitly from all co-authors, as well as from the responsible authorities. All authors whose names appear on the submission have contributed sufficiently to the scientific work. We ensure the correct author group, corresponding author, and order of authors at submission.

Supplementary material

10658_2018_1549_MOESM1_ESM.xlsx (22 kb)
Online resource 1 . Data of races, virulence occurrence frequency of 511 (in 2008) and 109 (in 2010) isolates (XLSX 22 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • L. Y. Zhang
    • 1
  • Q. F. Meng
    • 1
  • H. F. Yan
    • 1
    Email author
  • D. Q. Liu
    • 1
  1. 1.College of Plant Protection, Agricultural University of Hebei, Biological Control Center of Plant Diseases and Plant Pests of Hebei ProvinceNational Engineering Research Center for Agriculture in Northern Mountainous AreasBaodingChina

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