Virulence and molecular genetic diversity of the Puccinia triticina population in Hebei Province of China in 2008 and 2010
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Wheat leaf rust, caused by Puccinia triticina, is a commonly occurring disease in wheat growing areas of the world. Research on the virulence and genetic diversity of P. triticina will be helpful for a better understanding of the evolution tendencies and subsequently for controlling this disease. In this study, 59 isolates of P. triticina collected from Hebei Province of China in 2008 and 2010 were analyzed for virulence diversity based on 37 near-isogenic Thatcher wheat lines at seedling stage, and genetic diversity using 21 pairs of EST-SSR primers. The predominant virulence phenotypes were PHTT, THTT, and THJT in 2008, while THTS, THSS, THTT, and THST were most common in Hebei province in 2010. Clustering analysis based on the virulence and EST-SSR data revealed a high diversity of P. triticina, and differences between the populations of 2008 and 2010. There was no significant correlation between the molecular and virulence data. Genetic parameters analysis also showed high diversity of the P. triticina population in Hebei province. Nei’s gene diversity index (H) and Shannon’s information index (I) showed that the genetic diversity of population 2008 was higher than 2010. The AMOVA analysis suggested that genetic variation mainly from intra-population (61.11%).
KeywordsWheat leaf rust EST-SSR Virulence Genetic diversity
The authors thank Ian Dundas (Adelaide University, Australia) for critical reading of the manuscript. This research was supported by National Key Basic Research Program of China (2013CB127700), and Special Fund for Natural Science Fund Project of Hebei Province (C2015204105).
This study was funded by 2013CB127700 and C2015204105.
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Conflicts of interest
The authors declare that they have no conflict of interest.
Human and animal rights
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.
Informed consent was obtained from all individual participants included in the study.
The manuscript has not been submitted to more than one journal for simultaneous consideration, and has not been published previously. This is a single study not being split up into several parts and not being submitted to various journals or to one journal over time. No data have been fabricated or manipulated to support our conclusions. We don’t plagiarism others’ data, text, or theories. Consent to submit has been received explicitly from all co-authors, as well as from the responsible authorities. All authors whose names appear on the submission have contributed sufficiently to the scientific work. We ensure the correct author group, corresponding author, and order of authors at submission.
- Bhardwaj, S. C., Prashar, M., Jain, S. K., Kumar, S., & Sharma, Y. P. (2010). Physiologic specialization of Puccinia triticina on wheat (Triticum species) in India. Indian Journal of Agricultural Science, 80(9), 805–811.Google Scholar
- Chen, W. Q., Qin, Q. M., Wang, K. R., Xia, S. B., Feng, G. H., & Chen, R. Z. (1997). Genes for leaf and stem rust resistance in important wheat cultivars in Jiangsu province. Journal of Plant Protection, 24, 22–233.Google Scholar
- Chen, W. Q., Qin, Q. M., Chen, Y. L., & Yan, S. B. (1998). Virulence dynamics of Puccinia recondita f.sp tritici in China during 1992~1996. Acta Phytopathologica Sinica, 28(2), 101–106.Google Scholar
- Chen, J., Lv, Z. Z., Xu, J. J., & Zhou, H. M. (2013). Analysis of physical and chemical properties of wheat in our producing area. Cereal & Feed Industry, 2, 6–9.Google Scholar
- Dadrezaie, S. T., Lababidi, S., Nazari, K., Goltapeh, E. M., Afshari, F., Alo, F., Shams-Bakhsh, M., & Safaie, N. (2013). Molecular genetic diversity in Iranian populations of Puccinia triticina, the causal agent of wheat leaf rust. American Journal of Plant Sciences, 4(7), 1375–1386.CrossRefGoogle Scholar
- de Vallavieille-Pope, C., Ali, S., Leconte, M., Enjalbert, J., Delos, M., and Rouzet, J. (2012). Virulence dynamics and regional structuring of Puccinia striiformis f. sp. tritici in France between 1984 and 2009. Plant Disease, 96(1), 131–140.Google Scholar
- Gao, J. (2003). Genetic polymorphism of wheat leaf rust (Puccinia recondita f.sp. tritici) based on virulence and AFLP. Dissertation, Agricultural University of Hebei, China.Google Scholar
- Germán, S., Barcellos, A., Chaves, M., Kohli, M., Campos, P., and de Viedma, L. (2007). The situation of common wheat rusts in the southern cone of America and perspectives for control. Crop & Pasture Science, 58(6), 620–630.Google Scholar
- Morgounov, A., Tufan, H. A., Sharma, R., Akin, B., Bagci, A., Braun, H. J., Kaya, Y., Keser, M., Payne, T. S., Sonder, K., & McIntosh, R. (2012). Global incidence of wheat rusts and powdery mildew during 1969–2010 and durability of resistance of winter wheat variety Bezostaya 1. European Journal of Plant Pathology, 132(3), 323–340.CrossRefGoogle Scholar
- Ordoñez, M. E., German, S. E., & Kolmer, J. A. (2010). Genetic differentiation within the Puccinia triticina population in South America and comparison with the north American population suggests common ancestry and intercontinental migration. Phytopathology, 100(4), 376–383.CrossRefPubMedGoogle Scholar
- Pu, Z. G., Liu, T. G., Zhang, M., & Chen, W. Q. (2004). Development of a molecular detection assay for the physiological race MFR of Puccinia triticina. Acta Phytopathologica Sinnica, 34(5), 449–454.Google Scholar
- Wan, J. J. (2010). Pathogenic types and virulence frequency of Puccinia triticina in Hebei, Henan and Shandong provinces. Dissertation, Agricultural University of Hebei, Baoding, Hebei, China.Google Scholar
- Wang, H. R. (1940). Notes on physiologic specialization in leaf rust on wheat in China. Phytopathology, 37, 680–690.Google Scholar
- Wen, X. L. (2008). Pathogenic types and virulence genes frequency of Puccinia triticina in China in 2006. Dissertation, Agricultural University of Hebei, Baoding, Hebei, China.Google Scholar
- Wu, Y. C. (2009). Pathogenic types and virulence frequency of Puccinia triticina in China in 2007. Dissertation, Agricultural University of Hebei, Baoding, Hebei, China.Google Scholar
- Xu, D. Q., Huang, G. H., Yang, W. X., & Liu, D. Q. (2002). Analysis of the virulence and molecular polymorphism of Puccinia recondita f.sp tritici. Journal of Agricultural Biotechnology, 10(1), 41–45.Google Scholar
- Xu, M. Q., Wang, S., & Meng, Q. F. (2013). Genetic diversity of Puccinia triticina by SSR in some regions of China. Journal of Agricultural Biotechnology, 21(1), 89–96.Google Scholar
- Yang, W. X., Meng, Q. F., Feng, S. D., & Liu, D. Q. (2004). Virulence of Puccinia triticina on wheat in China in 1999. Journal of Plant Protection, 31(1), 45–50.Google Scholar
- Zhang, J. Q., Liu, B., Chen, W. Q., Liu, T. G., & Gao, L. (2013). Temperature-sensitivity of population of Puccinia striiformis Westend. Acta Phytopathologica Sinnica, 43(1), 88–90.Google Scholar
- Zhang, H. S., Wei, X. J., Du, D. D., Wang, F., Yang, W. X., & Liu, D. Q. (2014). The distribution of leaf rust resistant gene Lr34 in 46 wheat breeding materials and their resistant performance. Journal of Triticeae Crops, 34(1), 28–33.Google Scholar