Occurrence of Apple stem grooving virus on Rubus ellipticus, a perennial weed in India
Rubus ellipticus, widely distributed in the Himalayas, was found to display severe virus-like symptoms (chlorosis, chlorotic mosaic, leaf deformation, necrotic and chlorotic spots on leaves). It was found growing abundantly in the campus of CSIR- IHBT Palampur, an important area for apple nursery development. Therefore to understand the status of this weed in terms of Apple stem grooving virus, leaf samples were tested. Seven samples from a total of thirty samples were found positive by DAS-ELISA, NASH and RT-PCR, confirming the presence of ASGV infection in 23% of the samples. Four of the positive samples (with positive signal in NASH) were characterized at coat protein (CP) gene. Phylogenetically, the CP sequences of the four isolates clustered with apple isolate (Ap-RC) from India, sharing 98.7–99.1% (aa) and 99.4–99.7% homology (nt). The rubus isolates shared an overall identity of 92.4–99.1% at (aa) and 86.6–99.5% at (nt) level with rest of the isolates from different hosts and geographical regions. R. ellipticus, might act as a reservoir host for ASGV, due to its widespread presence in the Himalayas.
KeywordsASGV DAS-ELISA NASH RT-PCR R. ellipticus
The authors are thankful to the Director, CSIR-Institute of Himalayan Bioresource Technology Palampur, HP (India), for providing necessary facilities. Department of Biotechnology (DBT), (grant no. BT/PR/11001PBD/16/803/2008), Govt. of India is duly acknowledged for financial support. Academy of Scientific and Innovative Research (AcSIR) New Delhi is also acknowledged. This is CSIR-IHBT publication number 3844.
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The authors declare that they do not have conflicts of interest.
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