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European Journal of Plant Pathology

, Volume 152, Issue 3, pp 833–840 | Cite as

Characterization and pathogenicity of sweet potato (Ipomoea batatas) black rot caused by Ceratocystis fimbriata in Korea

  • Narayan Chandra Paul
  • Sang-Sik Nam
  • Aardra Kachroo
  • Yun-Hee Kim
  • Jung-Wook Yang
Article
  • 146 Downloads

Abstract

Black rot disease was observed in sweet potato in Korea during a disease monitoring survey in 2017. The symptoms were black, small, circular, and slightly sunken lesions in the initial stages, leading to enlarged black spots and perithecia with long necks that appeared as dark bristles in the later stages. The causative agent was isolated on potato dextrose agar (PDA) and stored at the Sweet Potato Research Laboratory, Bioenergy Crop Research Institute, RDA, Muan (SPL17100 and SPL17101). The cultured organism produced cylindrical single-celled conidia after 5 days of incubation at 25 °C with a conidial size of 8.5–65.2 × 2.6–7.3 μm. Brownish, globose, and thick-walled aleuroconidia were produced with an 11.9–17.0 × 8.3–12.2 μm size. The perithecia were globose and dark brown with long thin necks tapering from the base to the apex. Morphologically, the fungus was identified as Ceratocystis fimbriata, which was well supported by the combined phylogenetic analysis of the sequences of the internal transcribed spacer (ITS), large subunit (LSU), and elongation factor 1-α (EF-1α) genes. Pathogenicity tests were conducted, and Koch’s postulates were confirmed.

Keywords

Ceratocystis fimbriata Molecular phylogeny Morphology Pathogenicity Sweet potato 

Notes

Acknowledgements

This research was supported by the Basic Research Program (Project No. PJ01207601) funded by the Rural Development Administration, Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal studies

No human participants and/or animals were involved in this research content.

Informed consent

This manuscript has not been published or presented elsewhere in part or in entirety and is not under consideration by another journal. We have read and understood your journal policies, and we believe that neither the manuscript nor the study violates any of these. All the authors have been personally and actively involved in substantive work leading to the manuscript and will hold themselves jointly and individually responsible for its content. All co-authors agreed to submit the article to the ‘European Journal of Plant Pathology’.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Narayan Chandra Paul
    • 1
  • Sang-Sik Nam
    • 1
  • Aardra Kachroo
    • 2
  • Yun-Hee Kim
    • 3
  • Jung-Wook Yang
    • 1
  1. 1.Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development AdministrationMuanRepublic of Korea
  2. 2.Department of Plant PathologyUniversity of KentuckyLexingtonUSA
  3. 3.Department of Biology Education, IALSGyeongsang National UniversityJinjuSouth Korea

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