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European Journal of Plant Pathology

, Volume 152, Issue 3, pp 691–700 | Cite as

Conidial sporulation of Stemphylium solani under laboratory conditions and infectivity of the inoculum produced in vitro

  • Fernanda C. de Souza
  • Kaoany F. da Silva
  • Silvaldo F. da Silveira
  • Lígia S. Kowata-Dresch
  • Carlos A. dos Santos
  • Margarida G. F. do Carmo
Article
  • 131 Downloads

Abstract

Stemphylium solani is an important foliar pathogen that infects many agricultural plants, especially solanaceous plants. The difficulty inducing sporulation in pure culture is a limiting factor for research on the different pathosystems involving S. solani. In this study, the influence of the culture medium, photoperiod with alternating temperatures, Petri dish cover materials (glass, polystyrene and PVC film) and stress factors of the colonies were investigated in the conidia production. Six sequential assays were performed with four isolates of S. solani, obtained from tomato plants. The inoculum produced was evaluated for infectivity on tomato plants under greenhouse conditions. The addition of fresh tomato juice to the agar medium and incubation temperatures of approximately 25 °C favoured mycelial growth. The ability to sporulate in vitro varied with the isolate, but in general the conidia production was significantly higher in V8 medium at 25 °C 6 h−1 light and 10 °C 18 h−1 of darkness. Sporulation was lower in glass Petri dishes but higher in transparent polystyrene dishes. The methodology allowed the production of viable and infective inoculum in sufficient quantity to inoculate plants under experimental conditions.

Keywords

Culture medium Temperature Photoperiod Conidia production 

Notes

Acknowledgements

The authors are grateful to the postgraduate course “Fitotecnia” of the “Universidade Federal Rural do Rio de Janeiro,” the “Coordenação de Aperfeiçoamento de Pessoal de Nível Superior” (CAPES) by the Master’s scholarship for the first author and to the “Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro” (FAPERJ) for their financial support.

Compliance with ethical standards

All authors are aware with the content of the manuscript and have agreed upon its submission to European Journal of Plant Pathology. 1) The manuscript has not been published in whole or in part elsewhere; 2) The manuscript is not currently being considered for publication in another journal; 3) The manuscript is not split up into several parts to increase the quantity of submissions;

Conflict of interest

No conflict of interest exits in the submission of this manuscript.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Fernanda C. de Souza
    • 1
  • Kaoany F. da Silva
    • 1
  • Silvaldo F. da Silveira
    • 2
  • Lígia S. Kowata-Dresch
    • 1
  • Carlos A. dos Santos
    • 1
  • Margarida G. F. do Carmo
    • 1
  1. 1.Departamento de Fitotecnia, Instituto de AgronomiaUniversidade Federal Rural do Rio de JaneiroSeropédicaBrazil
  2. 2.Laboratório de Entomologia e FitopatologiaUniversidade Estadual do Norte Fluminense Darcy RibeiroCampos dos GoytacazesBrazil

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