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European Journal of Plant Pathology

, Volume 152, Issue 4, pp 933–944 | Cite as

Assessing the phenotypic and genotypic diversity of Sclerotinia sclerotiorum in France

  • Christel Leyronas
  • Marc Bardin
  • Karine Berthier
  • Magali Duffaud
  • Claire Troulet
  • Marie Torres
  • François Villeneuve
  • Philippe C. Nicot
SI: Plant Pathology for Innovative Agroecology

Abstract

White mould caused by the ascomycete Sclerotinia sclerotiorum affects the production of many economically important crops. The incidence of this disease has recently increased in France, especially in melon crops, which were not affected much in the past. One possible explanation for this situation is the emergence of strains with particular characteristics, including increased aggressiveness to melon. To test this hypothesis, 200 isolates of S. sclerotiorum were collected from six host crops (bean, brassica oilseed rape, carrot, lettuce, melon, witloof chicory) in different regions. They were genotyped with 16 microsatellites markers. A subsample of 96 isolates were assessed for their aggressiveness on melon leaves. Overall, the isolates from melon did not show higher aggressiveness on melon leaves than those which originated from other host plants. Moreover, the melon isolates did not present distinctive genetic characteristics in comparison with those from other crops and shared several of the 128 identified multilocus haplotypes with isolates collected from carrot, witloof chicory and oilseed rape. Furthermore the Bayesian analysis of the genetic structure indicated that the host plant is not a structuring factor of the three genetic clusters identified, and it suggested instead the occurrence of an isolation-by-distance process. Possible consequences of these results for the management of white mould and alternative hypotheses to explain the recent changes in disease incidence are presented.

Keywords

White mould Microsatellites Aggressiveness Vegetable Melon 

Notes

Acknowledgements

This study was supported in part by a CASDAR grant of the French Ministry of Agriculture (SCLEROLEG Project) and by the Groupement d’Intérêt Scientifique pour la Production Intégrée des Cultures légumières” (GIS PIClég). The authors thank all the field experimenters of the technical institutes (ACPEL, APEF, CEFEL, INVENIO, Terres Inovia, SILEBAN, UNILET) who collaborated in the project and who collected the isolates used in the present study.

Compliance with ethical standards

The research presented in this paper was supported in part by a CASDAR grant of the French Ministry of Agriculture (SCLEROLEG Project) and by the Groupement d’Intérêt Scientifique pour la Production Intégrée des Cultures légumières” (GIS PIClég).

The authors declare that this research was performed according to the ethical standards.

All the authors agreed to submit this manuscript in this current version.

Conflict of interest

The authors declare to have no conflict of interest.

Human and animal rights and informed consent

The research did not involve human participants and/or animals.

Supplementary material

10658_2018_1493_MOESM1_ESM.docx (244 kb)
ESM 1 Results of the multivariate analysis performed by considering for each isolate the cluster number as a factor and the year of sampling as well as the geographic coordinates as continuous variables. (DOCX 244 kb)
10658_2018_1493_MOESM2_ESM.pdf (30 kb)
ESM 2 Results of the test for isolation-by-distance (PDF 29 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Christel Leyronas
    • 1
  • Marc Bardin
    • 1
  • Karine Berthier
    • 1
  • Magali Duffaud
    • 1
  • Claire Troulet
    • 1
  • Marie Torres
    • 2
  • François Villeneuve
    • 3
  • Philippe C. Nicot
    • 1
  1. 1.Pathologie VégétaleINRAMontfavetFrance
  2. 2.Ctifl, Centre de BalandranBellegardeFrance
  3. 3.Ctifl, Centre de LanxadePrigonrieuxFrance

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