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European Journal of Plant Pathology

, Volume 153, Issue 4, pp 1185–1202 | Cite as

Induction of resistance to Bambusa pervariabilis×Dendrocalamopsis grandis blight by protein AP-toxin and response of culturable microorganisms

  • Shujiang Li
  • Qianqian He
  • Qi Peng
  • Hanmingyue Zhu
  • Shuhan Li
  • Tianhui ZhuEmail author
Article
  • 66 Downloads

Abstract

The protein Arthrinium phaeospermum toxin (AP-toxin) was isolated from Arthrinium phaeospermum, which is the pathogen of bamboo blight. The present study demonstrated that disease resistance against bamboo blight (caused by A. phaeospermum) in Bambusa pervariabilis×Dendrocalamopsis grandis varieties could be induced by the application of inactivated protein AP-toxin (40 μg/mL, treated at 60 °C) to branches. Culturable microorganisms from three varieties of bamboo showed responses to various epiphytic and endophytic species and numbers after the application of inactivated protein AP-toxin. The plate dilution and tissue separation methods were used to separate the epiphytic and endophytic microorganisms of the three bamboo varieties from twigs collected at four different time points, and morphological characterization and molecular biology techniques were used to identify the fungi, bacteria and actinomyces species and record the quantities. The results showed that the numbers and species of epiphytic and endophytic microorganisms were significantly correlated with the disease index, treatment method (Treated: only AP-toxin, Treated+Infected: AP-toxin+A. phaeospermum, Infected: sterilized water+ A. phaeospermum, Control: only sterilized water) and resistance of the varieties. Moreover, the species of epiphytic bacteria were highly correlated to the resistance of the varieties. The above fingings indicate that the inactivated protein AP-toxin can be used as an inducible factor to change the response of a culturable microorganism and thereby improve the resistance of bamboo.

Keywords

Bambusa pervariabilis×Dendrocalamopsis grandis blight Disease resistance Induced effect Microbial response Protein AP-toxin 

Notes

Acknowledgements

This research was supported by the National Natural Science Foundation of China (31700568), the China Postdoctoral Science Foundation (2016 M602705) and College Students’ innovation and entrepreneurship training program of Sichuan Province (201810626117).

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Shujiang Li
    • 1
  • Qianqian He
    • 1
  • Qi Peng
    • 1
  • Hanmingyue Zhu
    • 2
  • Shuhan Li
    • 1
  • Tianhui Zhu
    • 1
    Email author
  1. 1.College of ForestrySichuan Agricultural UniversityChengduChina
  2. 2.College of Landscape ArchitectureSichuan Agricultural UniversityChengduChina

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