European Journal of Plant Pathology

, Volume 153, Issue 1, pp 169–180 | Cite as

Species diversity of Colletotrichum infecting Annona spp. in Brazil

  • Jaqueline F. O. CostaEmail author
  • Sandra H. Kamei
  • Janaíne Rossane A. Silva
  • Ana Raphaela G. da S. Miranda
  • Mariote B. Netto
  • Sarah Jacqueline C. da Silva
  • Kamila C. Correia
  • Gaus S. de Andrade Lima
  • Iraildes P. Assunção


Anthracnose disease caused by Colletotrichum species is a limiting factor in the production and commercialization of annonaceous crops in different regions of the world. In this study, 96 Colletotrichum isolates were collected from soursop and sugar apple leaves showing anthracnose symptoms in commercial plantations of the state of Alagoas, northeastern region of Brazil. To assess initially the species diversity, the isolates were identified by phylogeny based on the sequences of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. Representative strains were selected for further identification based on multigene phylogeny analysis using sequences of the internal transcribed spacer region (ITS), actin (ACT), chitin synthase (CHS-1), β-tubulin (TUB2), calmodulin (CAL), histone3 (HIS3), glutamine synthetase (GS) and (GAPDH) genes. Morpho-cultural characterization and pathogenicity tests were also performed. These analyses allowed the identification of nine species: Colletotrichum brevisporum, C. cliviae, C. fructicola, C. gigasporum, C. gloeosporioides, C. karstii, C. siamense, C. theobromicola and C. tropicale. Until now, only C. fructicola, C. gloeosporioides and C. theobromicola had been associated with anthracnose of soursop and sugar apple in Brazil. C. brevisporum, C. cliviae and C. gigasporum species are also described for the first time on annonaceous species worldwide.


Annona muricata Annona squamosa Colletotrichum brevisporum Colletotrichum cliviae Colletotrichum gigasporum Pathogenicity 



This work was supported by Fundação de Amparo à Pesquisa do Estado de Alagoas - FAPEAL. We also thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES for a doctoral fellowship.

Compilance with ethical standards

This research article is not submitted elsewere for publication and this manuscript complies with the Ethical Rules applicable for this jornal.

Conflict of interest

The authors declare that they have no competing interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10658_2018_1630_MOESM1_ESM.docx (29 kb)
ESM 1 (DOCX 28 kb)


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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Jaqueline F. O. Costa
    • 1
    Email author
  • Sandra H. Kamei
    • 1
  • Janaíne Rossane A. Silva
    • 1
  • Ana Raphaela G. da S. Miranda
    • 1
  • Mariote B. Netto
    • 1
  • Sarah Jacqueline C. da Silva
    • 1
  • Kamila C. Correia
    • 2
  • Gaus S. de Andrade Lima
    • 1
  • Iraildes P. Assunção
    • 1
  1. 1.Centro de Ciências AgráriasUniversidade Federal de AlagoasRio LargoBrazil
  2. 2.Centro de Ciências Agrárias e da BiodiversidadeUniversidade Federal do CaririCratoBrazil

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