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European Journal of Plant Pathology

, Volume 151, Issue 3, pp 781–790 | Cite as

Effects of pH and titratable acidity on the growth and development of Monilinia laxa (Aderh. & Ruhl.) in vitro and in vivo.

  • Vitus Ikechukwu Obi
  • Juan Jose Barriuso
  • Yolanda Gogorcena
Article

Abstract

This investigation examines the effects of pH and titratable acidity on the growth and developments of a strain of Monilinia laxa (Aderhold & Ruhland) at seven different pH levels in Potato Dextrose Agar media and on peach fruit from formation to commercial maturity. The fungi growth was obtained by daily measurement of mycelia on the pH amended Potato Dextrose Agar. The sporulation performance was determined after 30 days of culture incubation. Fruits were inoculated with M. laxa, from fruit set to maturity, on weekly basis for brown rot susceptibility. The pathogen development, in vitro, was affected, by the pH (2.4–11.52) amended nutrient media. M. laxa exhibited variation in its growth and sporulation capacities on the seven pH amended PDA, preferring relatively moderate acidic conditions for optimum performance. In the in vitro analysis, there was mycelia growth at pH 2.40 to 8.84, while pH 11.52 did not support any mycelia growth. There was a continuous and stable increase in weight of fruit as it developed whereas the fruit size increased, then decreased and finally increased as the fruit develops. The acidity dynamics exhibited a non-sinusoidal waveform through the growth and development of the fruit. In all these characteristic variations, M. laxa did not develop infection or shown any brown rot incidence in the fruit until the period of commercial maturity.

Keywords

Alkaline Monilia Physicochemical Prunus persica Tolerance 

Abbreviations

BRI

Brown rot incidence

DAF

Days after floration

DAS

Days after fruit set

EpHV

Expected pH values

FS

Fruit size

FW

Fruit weight

JDs

Julian days

PDA

Potato dextrose agar

PP

Polypropylene

RPM

Revolutions per minute

RpHV

Real pH values

TA

Titratable acid.

Notes

Acknowledgements

We thank Dr. J. Usall of the (IRTA Lleida) for providing the original inoculum of the M. laxa; to Dr. M.A. Moreno (EEAD-CSIC) for providing plant material; and to R. Giménez (EEAD-CSIC) for technical assistance. The Research Center and Food Technology of Aragón (CITA) allowed us the use of its plant protection facilities.

Funding

This work was financed by the MINECO and the Government of Aragón with projects AGL2014-52063R A44; co-financed with FEDER and ESF, respectively.

Compliance with ethical Standard

Conflict of interest

The authors have declared that there is no conflict of interest exists.

Research involving Human Participants and/or Animals

This article /manuscript does not contain any studies with human and /or animal subjects performed by any of the authors.

Informed consent

This article is as a result of a general study in the evaluation of peach germplasm for tolerance to brown rot by Monilinia laxa at the Aula Dei-CSIC peach collection, Zaragoza. I, Vitus Ikechukwu Obi, testify on behalf of all co-authors (Yolanda Gogorcena and Juan Jose Barriuso) that our article.

Title: “Effects of pH and titratable acidity on the growth and development of Monilinia laxa (Aderh. & Ruhl.) in vitro and in vivo”,

a) has not been published in whole or in part elsewhere;

b) is not currently being considered for publication in another journal;

c) and that all authors have been personally and actively involved in substantive work leading to the manuscript, and will hold ourselves jointly and individually responsible for its content.

Supplementary material

10658_2017_1413_MOESM1_ESM.pdf (197 kb)
ESM 1 (PDF 196 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • Vitus Ikechukwu Obi
    • 1
  • Juan Jose Barriuso
    • 2
  • Yolanda Gogorcena
    • 1
  1. 1.Estación Experimental de Aula Dei-CSICZaragozaSpain
  2. 2.Instituto Agroalimentario de Aragón IA2CITA-Universidad de ZaragozaZaragozaSpain

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