European Journal of Plant Pathology

, Volume 151, Issue 3, pp 677–688 | Cite as

Alternaria alternata, the causal agent of leaf blight of sunflower in South Africa

  • M. G. Kgatle
  • M. Truter
  • T. M. Ramusi
  • B. Flett
  • T. A. S. Aveling


Sunflower (Helianthus annuus L.) is an important oilseed crop in South Africa, and is grown in rotation with maize in some parts of North West, Limpopo, Free State, Mpumalanga and Gauteng provinces. Alternaria leaf blight is currently one of the major potential disease threats of sunflower and is capable of causing yield losses in all production regions. Alternaria helianthi was reported as the main cause of Alternaria leaf blight of sunflower in South Africa; however small-spored Alternaria species have been consistently isolated from leaf blight symptoms during recent surveys. The aim of this study was to use morphological and molecular techniques to identify the causal agent(s) of Alternaria blight isolated from South African sunflower production areas. Alternaria helianthi was not recovered from any of the sunflower lesions or seeds, with only Alternaria alternata retrieved from the symptomatic tissue. Molecular identification based on a combined phylogenetic dataset using the partial internal transcribed spacer regions, RNA polymerase second largest subunit, glyceraldehyde-3-phosphate dehydrogenase, translation elongation factor and Alternaria allergen gene regions was done to support the morphological identification based on the three-dimensional sporulation patterns of Alternaria. Furthermore, this study aimed at evaluating the pathogenicity of the recovered Alternaria isolates and their potential as causal agents of Alternaria leaf blight of sunflower. Pathogenicity tests showed that all the Alternaria alternata isolates tested were capable of causing Alternaria leaf blight of sunflower as seen in the field. This is the first report of A. alternata causing leaf blight of sunflower in South Africa.


Helianthus annuus Alternaria leaf blight Oilseed 



This study was funded by The Oil- and Protein Seeds Development Trust, South Africa.

Compliance with ethical standards

The research presented in this research does not implicate nor Human Participants and/or Animals.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Allen, S. J., Brown, J. F., & Kockman, J. K. (1983). Effect of leaf age, host growth stage, leaf injury and pollen on the infection of sunflower by Alternaria helianthi. Phytopathology, 73, 896–898.CrossRefGoogle Scholar
  2. Andersen, B., Kroger, E., & Roberts, G. (2002). Chemical and morphological segregation of Alternaria arborescens, A. infectoria and A. tenuissima species-groups. Mycological Research, 106(2), 170–182.CrossRefGoogle Scholar
  3. Andrew, M., Peever, T. L., & Pryor, B. M. (2009). An extended multilocus phylogeny does not resolve morphological species with the small-spored Alternaria species complex. Mycologia, 101(1), 95–109.CrossRefPubMedGoogle Scholar
  4. Aradhya, M. K., Chan, H. M., & Parfitt, D. E. (2001). Genetic variability in the pistachio late blight fungus, Alternaria alternata. Mycological Research, 5(3), 300–306.CrossRefGoogle Scholar
  5. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., & Struhl, K. (1998). Short protocols in molecular biology. New Jersey: Wiley.Google Scholar
  6. Calvet, N. P., Ungaro, M. R. G., & Oliveira, R. F. (2005). Virtual lesion of Alternaria blight on sunflower. Helia, 28(42), 89–100.CrossRefGoogle Scholar
  7. Fairchild, K. L., Miles, T. D., & Wharton, P. S. (2013). Assessing the fungicide resistance in populations of Alternaria in Idaho potato fields. Crop Protection, 49, 31–39.CrossRefGoogle Scholar
  8. Hall, T.A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.Google Scholar
  9. Kapsa, J.S. & Osowski, J. (2012). Host – pathogen interaction between Alternaria species and S. tuberosum under different conditions. Thirteenth Euroblight workshop: St. Petersburg, Russia. Special Report, 15, 107–112.Google Scholar
  10. Konstantinova, P., Bonants, P. J. M., Gent-Pelzer, M. P. E., Zouwen, P., & Bulk, R. (2002). Development of specific primers for detection and identification of Alternaria spp. in carrot material by PCR comparison with blotter and plating assays. Mycological Research, 106(1), 23–32.CrossRefGoogle Scholar
  11. Kumar, V., Haldar, S., Pandey, K. K., Singh, R. P., Singh, A. K., & Singh, P. C. (2008). Cultural, morphological, pathogenic and molecular variability amongst tomato isolate of Alternaria solani in India. World Journal of Microbiology and Biotechnology, 24, 1003–1009.CrossRefGoogle Scholar
  12. Kusaba, M., & Tsuge, T. (1995). Phylogeny of Alternaria fungi known to produce host-specific toxins on the basis on the variation in the internal transcribed spaces of the ribosomal DNA. Current Genetics, 28, 491–498.CrossRefPubMedGoogle Scholar
  13. Lapagodi, A. L., & Thanassoulopoulos, C. C. (1998). Effect of a leaf spot disease caused by Alternaria alternata on sunflower yield in Greece. Plant Disease, 82, 41–44.CrossRefGoogle Scholar
  14. Lawrence, D. P., Gannibal, P. B., Peever, T. L., & Pryor, B. M. (2013). The sections of Alternaria: formalising species-groups concepts. Mycologia, 105(3), 530–546.CrossRefPubMedGoogle Scholar
  15. Logrieco, A., Moretti, A., & Solfrizzo, M. (2009). Alternaria toxins and plant diseases: an overview of origin, occurrence and risks. World Mycotoxin Journal, 2(2), 129–140.CrossRefGoogle Scholar
  16. Lourenco Jr., V., Moya, A., Gonzalez-Candelas, F., Carbone, I., Maffia, L. A., & Mizubuti, E. S. G. (2009). Molecular diversity and evolutionary processes of Alternaria solani in Brazil inferred using genealogical and coalescent approaches. Phytopathology, 99, 765–774.CrossRefPubMedGoogle Scholar
  17. Meng, J. W., Zhu, W., He, M. H., Wu, E. J., Duan, G. H., Xie, Y. K., Jin, Y. J., Yang, L. N., Shang, L. P., & Zhan, J. (2015). Population genetic analysis reveals cryptic sex in the phytopathogenic fungus Alternata alternata. Scientific Reports, 5, 18250.CrossRefPubMedPubMedCentralGoogle Scholar
  18. Nishikawa, J., & Nakashima, C. (2013). Taxonomic characterization and experimental host ranges of four newly recorded species of Alternaria from Japan. Journal of Phytopathology, 161, 604–616.CrossRefGoogle Scholar
  19. Park, M. S., Romanoski, C. E., & Pryor, B. M. (2008). A re-examination of the phylogenetic relationship between the causal agents of carrot black rot, Alternaria radicina and A. carotiincultae. Mycologia, 100(3), 511–527.CrossRefPubMedGoogle Scholar
  20. Peever, T. L., Su, G., Carpenter-Boggs, L., & Timmer, L. W. (2004). Molecular systematic of citrus-associated Alternaria species. Mycologia, 96(1), 119–134.CrossRefPubMedGoogle Scholar
  21. Peever, T. L., Carpenter-Boggs, L., Timmer, L. W., Carris, L. M., & Bhatia, A. (2005). Citrus black rot is caused by phylogenetically distinct lineages of Alternaria alternata. Phytopathology, 95, 512–518.CrossRefPubMedGoogle Scholar
  22. Pryor, B. M., & Gilbertson, R. L. (2000). Molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear ITS and mt SSU rDNA sequences. Mycological Research, 104(11), 1312–1321.CrossRefGoogle Scholar
  23. Pryor, B. M., & Michailides, T. J. (2002). Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio. Phytopathology, 92, 406–416.CrossRefPubMedGoogle Scholar
  24. Rotem, J. (1994). The genus Alternaria: Biology, epidemiology and pathogenicity. APS Press: St Paul.Google Scholar
  25. SAS Institute Inc. (2013). Base SAS® 9.4 procedures guide: Statistical procedures. Cary: SAS Institute Inc..Google Scholar
  26. Simmons, E.G. (2007). Alternaria: An identification manual. CBS Biodiversity 6, CBS Fungal Biodiversity Centre, Utrecht, The Netherlands.Google Scholar
  27. Stammler, G., Bohme, F., Philippi, J., Miessner, S., & Tegge, V. (2014). Pathogenicity of Alternaria species on potatoes and tomatoes. Fourteenth EuroBlight Workshop: Limassol, Cyprus. Special Report, 16, 85–96.Google Scholar
  28. Swofford, D. L. (2002). PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates. Sunderland, Massachusetts.Google Scholar
  29. Thomma, B. P. H. J. (2003). Alternaria spp.: From general saprophytic to specific parasite. Molecular Plant Pathology, 4(4), 225–236.CrossRefPubMedGoogle Scholar
  30. van der Westhuizen, G. C. A., & Holtzhausen, M. A. (1980). Alternaria helianthi on sunflower in South Africa. Phytopathologica, 12, 49–52.Google Scholar
  31. Woudenberg, J. H. C., Groenewald, J. Z., & Crous, P. W. (2013). Alternaria redefined. Studies in Mycology, 75(1), 171–212.CrossRefPubMedPubMedCentralGoogle Scholar
  32. Woudenberg, J. H. C., Seidi, M. F., Groenewald, J. Z., de Vries, M., Stielow, J. B., Thomma, B. P. H. J., & Crous, P. W. (2015a). Alternaria section Alternaria: Species, formae speciales or pathotypes? Studies in Mycology, 82, 1–21.CrossRefPubMedPubMedCentralGoogle Scholar
  33. Woudenberg, J. H. C., van der Merwe, N. A., Jurjevic, Z., Groenewald, J. Z., & Crous, P. W. (2015b). Diversity and movement of indoor Alternaria alternata across the mainland USA. Fungal Genetics and Biology, 81, 62–72.CrossRefPubMedGoogle Scholar
  34. Zhu, X. Q., & Xiao, C. L. (2015). Phylogenetic, morphological and pathogenic characterization of Alternaria species associated with fruit rot of blueberry in California. Phytopathology, 105, 1555–1567.CrossRefPubMedGoogle Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2018

Authors and Affiliations

  • M. G. Kgatle
    • 1
  • M. Truter
    • 2
  • T. M. Ramusi
    • 3
  • B. Flett
    • 3
    • 4
  • T. A. S. Aveling
    • 1
  1. 1.Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology InstituteUniversity of PretoriaPretoriaSouth Africa
  2. 2.ARC-Vegetable and Ornamental PlantsPretoriaSouth Africa
  3. 3.ARC-Grain Crops InstitutePotchefstroomSouth Africa
  4. 4.Unit for Environmental Sciences and ManagementNorth-West UniversityPotchefstroomSouth Africa

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