Laimaphelenchus suberensis sp. nov. associated with Quercus suber in Portugal
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Laimaphelenchus suberensis sp. nov. obtained from declining Quercus suber trees of Herdade da Gouveia de Baixo, Alentejo, Portugal, is described and illustrated based on morphological, biometrical and molecular characters. The diagnosis of Laimaphelenchus species has been commonly based on the presence or absence of a vulval flap and on the shape structure of the tail tip. The species described here has been included in the Laimaphelenchus group without vulval flap, and can be distinguished from morphologically similar species by its tail tip shape structure that has a stalk-like terminus and three diffuse tubercles with 4–6 finger-like protrusions. For the molecular analyses, the mitochondrial DNA region from the cytochrome oxidase subunit I (mtCOI), the D2-D3 expansion segments of the large subunit (LSU) and small subunit (SSU) of rRNA gene were amplified and sequenced. Sequences of L. suberensis sp. nov. clustered separately from all Laimaphelenchus spp. with available sequences in Genbank, confirming its identification as a new species. This is the second report of the genus Laimaphelenchus in Portugal, associated with Q. suber: L. heidelbergi and L. suberensis sp. nov.
KeywordsCytochrome oxidase subunit I Cork oak Large subunit ribosomal RNA Molecular analysis Morphology Small subunit ribosomal RNA
This research was supported by CFE, CIEPQPF and FEDER funds through the ‘Programa Operacional Factores de Competitividade – COMPETE’ and by national funds through FCT–Fundação para a Ciência e a Tecnologia under the projects UID/BIA/04004/2013, PEst-C/EQB/UI0102/2013 and FCOMP-01-0124-008937 (Ref. PTDC/BIA–BEC/102834/2008) and by Instituto do Ambiente, Tecnologia e Vida (IATV). Carla Maleita (SFRH/BPD/85736/2012) and Sofia Costa (SFRH/BPD/102438/2014) were financed by MEC National funding and The European Social Fund through POCH (Programa Operacional Capital Humano).
Compliance with ethical standards
Conflict of interest
The authors certify that do not have any actual or potential conflict of interest, the study described is original, has not been published previously, and is not under consideration for publication elsewhere. All authors have reviewed the manuscript and approved the final version of manuscript before submission.
Research involving human participants and/or animals
No specific permits were required for the described studies. Permission for sampling the cork oak farms was granted by the landowner.
The authors certify that the work carried out in this research followed the principles of ethical and professional conduct.
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