Multilocus sequence analysis supports a low genetic diversity among ‘Candidatus Phytoplasma australasia’ related strains infecting vegetable crops and periwinkle in Egypt
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‘Candidatus Phytoplasma australasia’ causes important damages to the Egyptian vegetable crop production. A prerequisite for controlling the different diseases it causes to eggplant, tomato and squash, is to trace its propagation pathways. To allow the differentiation of ‘Ca. P. australasia’ strains, a multilocus sequence analysis protocol was developed. Four conserved phytoplama genes namely tuf, secY, dnaK and dppA, were selected among the CDS of a ‘Ca. P. aurantifolia’ genome draft. The corresponding genes were PCR amplified from tomato, eggplant and squash collected in 2010 from the governorates Sharkia, Elmynia and Beni sueif, as well as from Catharanthus roseus periwinkles collected in 2013 from Kafrelsheikh governorate. Sequence comparisons showed no diversity among the Egyptian isolates of ‘Ca. P. australasia’ that also constitute a distinct cluster within the 16SrII-D taxonomic subgroup. This low diversity supports a common epidemiology for the different diseases affecting vegetable crops and periwinkle in Egypt and suggests that future investigations on insect vector should focus on polyphagous leafhoppers.
KeywordsCucurbit Eggplant Tomato Periwinkle MLSA
Authors thank Sandrine Eveillard for helpful discussions and critical review of the manuscript. The whole Genome Shotgun was produced in the frame of the Phytoplasma Genome Sequencing Initiative (PGSI) of the European COST action FA0807 - Integrated management of phytoplasma epidemics in different crop systems.
AFO and XF and conceived and designed the study. AFO, MMS and SAM collected samples. YES, AFO, SAM and MMS performed the experiments. XF carried out the data analysis. XF and AFO contributed to the writing of the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All authors read and approved the final manuscript.
Human studies and participants
There was no involvement of human participants and/or animals in the present study.
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