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European Journal of Plant Pathology

, Volume 150, Issue 1, pp 179–190 | Cite as

Evaluation of oilseed rape seed yield losses caused by Leptosphaeria biglobosa in central China

  • Xiang Cai
  • Yongju Huang
  • Daohong Jiang
  • Bruce D.L. Fitt
  • Guoqing Li
  • Long YangEmail author
Article

Abstract

Phoma stem canker of oilseed rape (Brassica napus), caused by Leptosphaeria maculans/L. biglobosa is a globally important disease. Severe phoma stem canker symptoms have been observed on winter oilseed rape in China but the seed yield loss caused by this disease remains unknown. In May 2012 and May 2013, 17 and 13 crops were surveyed, respectively, in seven counties of Hubei Province, central China. Stems with phoma stem canker disease symptoms were sampled for pathogen isolation and identification. Only L. biglobosa was identified by culture morphology and species-specific PCR; no L. maculans was found. To evaluate the yield losses, yield components (number of branches per plant, number of pods per plant, 1000-seed weight, number of seeds per pod) were assessed on healthy and diseased plants sampled from crops in four counties and on plants from inoculated pot experiments (plants of three cultivars were inoculated at the green bud stage by injecting L. biglobosa conidia into the stem between the first and second leaf scars). Results of the field surveys showed that diseased plants had 14–61% less branches and 32–83% less pods than healthy plants, respectively. The estimated seed yield loss varied from 10% to 21% and from 13% to 37% in 2012 and 2013, respectively. In the pot experiments, there were no differences in numbers of branches or pods but there were differences in number of seeds per pod between inoculated and control plants. For the three cultivars tested, the inoculated plants had yield losses of 29–56% compared with the control. This study indicates that L. biglobosa could cause substantial seed yield loss in China.

Keywords

Brassica napus Blackleg Disease control Crop disease survey 

Notes

Acknowledgements

We thank the China Agriculture Research System (Grant CARS-13), China National Science and Technology Supporting Program (Grant No. 2010BAD01B04), the UK Biotechnology and Biological Sciences Research Council (BBSRC), AHDB Cereals and Oilseeds and the Felix Thornley Cobbold Agricultural Trust for supporting the work.

References

  1. Cai, X., Yang, L., Zhang, J., & Li, G. Q. (2014a). First report of Leptosphaeria biglobosa causing blackleg on Brassica campestris ssp. chinensis var. purpurea in central China. Plant Disease, 98, 1156.Google Scholar
  2. Cai, X., Yang, L., Zhang, J., & Li, G. Q. (2014b). First report of Leptosphaeria biglobosa causing blackleg on Raphanus sativus in central China. Plant Disease, 98, 993.CrossRefGoogle Scholar
  3. Dai, F. L. (1979). Flora Fungoru Sinicorum (pp. 1027–1028). Beijing, China: Science Press.Google Scholar
  4. Delourme, R., Chèvre, A. M., Brun, H., Rouxel, T., Balesdent, M. H., Dias, J. S., et al. (2006). Major gene and polygenic resistance to Leptosphaeria maculans in oilseed rape (Brassica napus). European Journal of Plant Pathology, 114, 41–52.CrossRefGoogle Scholar
  5. Fitt, B. D. L., Brun, H., Barbetti, M. J., & Rimmer, S. R. (2006a). World-wide importance of phoma stem canker (Leptosphaeria maculans and L. biglobosa) on oilseed rape (Brassica napus). European Journal of Plant Pathology, 114, 3–15.CrossRefGoogle Scholar
  6. Fitt, B. D. L., Huang, Y. J., van den Bosch, F., & West, J. S. (2006b). Coexistence of related pathogen species on arable crops in space and time. Annual Review of Phytopathology, 44, 163–182.CrossRefGoogle Scholar
  7. Fitt, B. D. L., Hu, B. C., Li, Z. Q., Liu, S. Y., Lange, R. M., Kharbanda, P. D., et al. (2008). Strategies to prevent spread of Leptosphaeria maculans (phoma stem canker) onto oilseed rape crops in China; costs and benefits. Plant Pathology, 57, 652–664.CrossRefGoogle Scholar
  8. Gladders, P., & Musa, T. (1979). The development of Leptosphaeria maculans in winter oilseed rape and its implications for disease control. Proceedings of the British Crop Protection Conference-Pests and Diseases, 129–136.Google Scholar
  9. Gugel, R., & Petrie, G. (1992). History, occurrence, impact, and control of blackleg of rapeseed. Canadian Jorunal of Plant Pathology, 14, 36–45.CrossRefGoogle Scholar
  10. Huang, Y. J., Toscano-Underwood, C., Fitt, B. D. L., Hu, X. J., & Hall, A. M. (2003). Effects of temperature on ascospore germination and penetration of oilseed rape (Brassica napus) leaves by A- or B-group Leptosphaeria maculans (phoma stem canker). Plant Pathology, 52, 245–255.CrossRefGoogle Scholar
  11. Huang, Y. J., Fitt, B. D. L., Jedryczka, M., Dakowska, S., West, J. S., Gladders, P., et al. (2005). Patterns of ascospore release in relation to phoma stem canker epidemiology in England (Leptosphaeria maculans) and Poland (Leptosphaeria biglobosa). European Journal of Plant Pathology, 111, 263–277.CrossRefGoogle Scholar
  12. Huang, Y. J., Qi, A. M., King, G. J., & Fitt, B. D. L. (2014). Assessing quantitative resistance against Leptosphaeria maculans (phoma stem canker) in Brassica napus (oilseed rape) in young plants. PloS One, 9, e84924.CrossRefGoogle Scholar
  13. Jedryczka, M., Nikonorenkov, V. A., Levitin, M., Gasich, E., Lewartowska, E., & Portenko, L. (2002). Spectrum and severity of fungal diseases on spring oilseed rape in Russia. IOBC WPRS Bulletin, 25, 13–20.Google Scholar
  14. Karolewski, Z., Kosiada, T., Hylak-Nowosad, B., & Nowacka, K. (2002). Changes in population structure of Leptosphaeria maculans in Poland. Phytopathologia Polonia, 25, 27–34.Google Scholar
  15. Lacoste, L., Louvet, J., Anselme, C., Alabouvette, C., Brunin, B., & Pierre, J. (1969). Rôle de Phoma lingam (Tode) Desm. et de sa forme parfaite, Leptosphaeria maculans (Desm.) Ces. et de Not. dans les épidémies de nécrose du collet de colza (Brassica napus L. var. oleifera Metzer). Comptes Rendus de l'Academie d'Agriculture de France, 981–987.Google Scholar
  16. Larkan, N. J., Ma, L., & Borhan, M. H. (2015). The Brassica napus receptor-like protein RLM2 is encoded by a second allele of the LepR3/Rlm2 blackleg resistance locus. Plant Biotechnology Journal, 13, 983–992.CrossRefGoogle Scholar
  17. Li, C. X., Wratten, N., Salisbury, P. A., Burton, W. A., Potter, T. D., Walton, G., et al. (2008). Response of Brassica napus and B. juncea germplasm from Australia, China and India to Australian populations of Leptosphaeria maculans. Australasian Plant Pathology, 37, 162–170.CrossRefGoogle Scholar
  18. Li, Q. S., Rong, S. B., Hu, B. C., Jiang, Y. F., Hou, S. M., Fei, W. X., et al. (2013). Distribution of blackleg disease on oilseed rape in China and its pathogen identification. Chinese Journal of Oil Crop Sciences, 35, 415–423.Google Scholar
  19. Liu, S. Y., Liu, Z., Fitt, B. D. L., Evans, N., Foster, S. J., Huang, Y. J., et al. (2006). Resistance to Leptosphaeria maculans (phoma stem canker) in Brassica napus (oilseed rape) induced by L. biglobosa and chemical defence activators in field and controlled environments. Plant Pathology, 55, 401–412.CrossRefGoogle Scholar
  20. Liu, Z., Latunde-Dada, A., Hall, A., & Fitt, B. D. L. (2014). Phoma stem canker disease on oilseed rape (Brassica napus) in China is caused by Leptosphaeria biglobosa ‘brassicae’. European Journal of Plant Pathology, 140, 841–857.CrossRefGoogle Scholar
  21. Marcroft, S. J., Sosnowski, M. R., Scott, E. S., Ramsey, M. D., Salisbury, P. A., & Howlett, B. J. (2005). Brassica napus Plants infected by Leptosphaeria maculans after the third to fifth leaf growth stage in south-eastern Australia do not develop blackleg stem canker. European Journal of Plant Pathology, 112, 289–292.CrossRefGoogle Scholar
  22. Möller, E. M., Bahnweg, G., Sandermann, H., & Geige, H. H. (1992). A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Research, 20, 6115–6116.CrossRefGoogle Scholar
  23. Rimmer, R., & Berg, V. D. (2007). Blackleg (phoma stem canker). In S. R. Rimmer, V. I. Shattuck, & L. Buchwaldt (Eds.), Compendium of brassica diseases (pp. 19–22). St Paul, MN, USA: APS Press.Google Scholar
  24. Rong, S. B., Hu, B. C., Chen, F. X., Wu, X. J., Hou, S. M., Fei, W. X., et al. (2015). Effects of Leptosphaeria biglobosa on grain yield and yield related traits of oilseed rape. Crops, 169, 159–161.Google Scholar
  25. Shoemaker, R. A., & Brun, H. (2001). The teleomorph of the weakly aggressive segregate of Leptosphaeria maculans. Canadian Journal of Botany, 79, 412–419.Google Scholar
  26. Song, P. L., Jedryczka, M., Irzykowski, W., Yan, M. J., Huangfu, H. Y., Hao, L. F., et al. (2016). Efficient detection of Leptosphaeria maculans from infected seed lots of oilseed rape. Journal of Phytopathology, 164, 1097–1104.CrossRefGoogle Scholar
  27. Toscano-Underwood, C, Huang, Y.J, Fitt, B.D.L., & Hall, A.M. (2003). Effects of temperature on maturation of pseudothecia of Leptosphaeria maculans and L. biglobosa on oilseed rape stem debris. Plant Pathology, 52, 726–736.Google Scholar
  28. Van Der Spek, J. (1981). Blackleg of oilseed rape in Netherlands. Medelelingen van de Faculteit Landbouwwetenschappen Rijksuniversiteit Gent, 46, 813–822.Google Scholar
  29. West, J. S., Evans, N., Liu, S., Hu, B., & Peng, L. (2000). Leptosphaeria maculans causing stem canker of oilseed rape in China. Plant Pathology, 49, 800.CrossRefGoogle Scholar
  30. West, J. S., Kharbanda, P. D., Barbetti, M. J., & Fitt, B. D. L. (2001). Epidemiology and management of Leptosphaeria maculans (phoma stem canker) on oilseed rape in Australia, Canada and Europe. Plant Pathology, 50, 10–27.CrossRefGoogle Scholar
  31. West, J. S., Balesdent, M. H., Rouxel, T., Narcy, J. P., Huang, Y. J., Roux, J., et al. (2002). Colonization of winter oilseed rape tissues by a/Tox(+) and B/Tox(0) Leptosphaeria maculans (phoma stem canker) in France and England. Plant Pathology, 51, 311–321.CrossRefGoogle Scholar
  32. Zhang, X., White, R. P., Demir, E., Jedryczka, M., Lange, R. M., Islam, M., et al. (2014). Leptosphaeria spp., phoma stem canker and potential spread of L. maculans on oilseed rape crops in China. Plant Pathology, 63, 598–612.CrossRefGoogle Scholar
  33. Zhou, Y., Fitt, B. D. L., Welham, S. J., Gladders, P., Sansford, C., & West, J. S. (1999). Effects of severity and timing of stem canker (Leptosphaeria maculans) symptoms on yield of winter oilseed rape (Brassica napus) in the UK. European Journal of Plant Pathology, 105, 715–728.CrossRefGoogle Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2017

Authors and Affiliations

  • Xiang Cai
    • 1
    • 2
  • Yongju Huang
    • 3
  • Daohong Jiang
    • 1
  • Bruce D.L. Fitt
    • 3
  • Guoqing Li
    • 1
  • Long Yang
    • 1
    Email author
  1. 1.The State Key Lab of Agricultural Microbiology and Hubei Key Laboratory of Plant PathologyHuazhong Agricultural UniversityWuhanChina
  2. 2.Institute of Crop Science of Wuhan Academy of Agriculture Science and TechnologyWuhanChina
  3. 3.School of Life and Medical SciencesUniversity of HertfordshireHatfieldUK

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