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European Journal of Plant Pathology

, Volume 150, Issue 1, pp 115–125 | Cite as

Trichomes: interaction sites of tomato leaves with biotrophic powdery mildew pathogens

  • Tomoko Suzuki
  • Tomoe Murakami
  • Yoshihiro Takizumi
  • Hiroyuki Ishimaru
  • Daiki Kudo
  • Yoshihiro Takikawa
  • Yoshinori Matsuda
  • Koji Kakutani
  • Yuling BaiEmail author
  • Teruo NonomuraEmail author
Article

Abstract

The present study aimed to explore the possibility of using the type I trichomes of tomato (Solanum lycopersicum) to monitor the infection processes of powdery mildews by microscopy. Individual trichome cells of two tomato genotypes were inoculated with pathogenic and non-pathogenic powdery mildew species, Pseudoidium neolycopersici, Erysiphe trifoliorum and Podosphaera xanthii. On the trichome cells of the tomato cultivar Moneymaker, hyphae of the pathogenic Pseudoidium neolycopersici (isolates KTP-03 and KTP-04) grew vigorously; whereas hyphal growth of the non-pathogenic Erysiphe trifoliorum and Podosphaera xanthii ceased after appressorium formation, which was associated with papilla formation and hypersensitive cell death, respectively. Similar infection processes of the tested powdery mildews were seen in Moneymaker epidermal cells. Therefore, tomato trichomes are suitable for analysing, at individual cell level, the infection processes of different pathotypes of powdery mildews and for observing the cytological responses of plants by non-pathogenic powdery mildews. On the other hand, it was observed that both isolates KTP-03 and KTP-04 failed to produce conidiophores on the hyphae elongating on Moneymaker trichomes. Similarly, no conidiophores were produced on the hyphae elongating on trichomes of Solanum peruvianum LA2172, which is resistant to KTP-03 and susceptible to KTP-04. Interestingly, delayed cell death occurred in LA2172 epidermal cells, which were attacked by KTP-03 hyphae elongating from trichomes and conidiophores were formed on new hyphae growing from the leaf epidermal cells. Thus, leaf trichomes and epidermal cells of the wild tomato species LA2172 reacted differently to the avirulent isolate KTP-03.

Keywords

Pseudoidium neolycopersici Erysiphe trifoliorum Podosphaera xanthii Hypersensitive cell death Delayed cell death Papilla formation 

Notes

Acknowledgements

This work was supported by a Grant for Scientific Research from Faculty of Agriculture, Kindai University.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Open Access

This article is distributed under the terms of the Creative Commons Attibution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2017

Authors and Affiliations

  • Tomoko Suzuki
    • 1
  • Tomoe Murakami
    • 2
  • Yoshihiro Takizumi
    • 2
  • Hiroyuki Ishimaru
    • 2
  • Daiki Kudo
    • 2
  • Yoshihiro Takikawa
    • 3
  • Yoshinori Matsuda
    • 2
  • Koji Kakutani
    • 4
  • Yuling Bai
    • 5
    Email author
  • Teruo Nonomura
    • 2
    Email author
  1. 1.Department of Chemical Biological Sciences, Faculty of ScienceJapan Women’s UniversityTokyoJapan
  2. 2.Laboratory of Phytoprotection, Science and Technology, Faculty of AgricultureKindai UniversityNaraJapan
  3. 3.Plant Center, Institute of Advanced TechnologyKindai UniversityWakayamaJapan
  4. 4.Pharmaceutical Research and Technology InstituteKindai UniversityOsakaJapan
  5. 5.Plant Breeding, Wageningen University & ResearchWageningenthe Netherlands

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