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European Journal of Plant Pathology

, Volume 148, Issue 4, pp 771–784 | Cite as

Nuclear proteome analysis of apple cultivar ‘Antonovka’ accessions in response to apple scab (Venturia inaequalis)

  • Sidona Sikorskaite-GudziunieneEmail author
  • Perttu Haimi
  • Dalia Gelvonauskiene
  • Vidmantas Stanys
Article
  • 282 Downloads

Abstract

This study presents the first comprehensive description of the differential nuclear proteome of apple leaves during the interaction with the fungal pathogen Venturia inaequalis. Nuclear proteins isolated from V. inaequalis infected leaves of three different apple genotypes, two ‘Antonovka’ accessions of varying resistance and cultivar ‘Puikis’ used as a control, were subjected to 2DE-DIGE. The analysis of proteins revealed 186 protein spots with significant differences in protein abundance (P ≤ 0.01), of which 67 proteins were identified through LC-MS/MS analysis. The proteins were classified into 9 functional categories that included redox regulation, cell signalling, cell homeostasis, protein degradation, epigenetic control, energy metabolism, photosynthesis, other stress-related proteins and proteins with unknown biological function. Comparison of the ‘Antonovka’ accessions revealed 13 proteins with different expression patterns. Among these were proteins mainly involved in ROS scavenging mechanisms, ubiquitin/26S proteasome-mediated protein degradation, protein folding and carbohydrate metabolism that may contribute to a varying resistance of ‘Antonovka’ accessions to apple scab. These results provide novel insights into the response of apple leaves to fungal pathogen infection and promote further investigation of the molecular mechanisms of apple resistance to V. inaequalis.

Keywords

‘Antonovka’ Apple Comparative proteomics 2DE-DIGE Nucleus V.inaequalis 

Notes

Acknowledgments

The research was part of the Lithuanian Research Centre for Agriculture and Forestry (LAMMC) long-term program „Genetics and purposeful change of genotypes of agricultural and forest plants“. The authors thank Dr. Danas Baniulis for help in analysing mass spectrometry data and his kind support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10658_2016_1131_MOESM1_ESM.pdf (301 kb)
Supplementary Fig. 1 Four biological replicate gels of nuclear proteins labeled with Cy3 or Cy5 of each apple genotype. (PDF 301 kb)
10658_2016_1131_MOESM2_ESM.pdf (363 kb)
Supplementary Table List of apple scab responsive apple nuclear proteins identified by MS/MS analysis. (PDF 363 kb)
10658_2016_1131_Fig4_ESM.gif (58 kb)
Supplementary Fig. 3

The scatterplot view of GO terms in the biological process ontology drawn by REVIGO. The scatterplot indicates the cluster representatives in a two-dimensional space derived by applying multidimensional scaling to a matrix of the GO terms’ semantic similarities. The cluster representatives are shown in distinct colour and other cluster members in dimmed colour. Bubble colour indicates provided p-value. Bubble radius indicates the generality of GO terms, where bigger bubbles imply more general terms. Scale on the right – the mean of log10 p value. (GIF 58 kb)

10658_2016_1131_MOESM3_ESM.tif (802 kb)
High resolution image (TIFF 802 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2016

Authors and Affiliations

  • Sidona Sikorskaite-Gudziuniene
    • 1
    Email author
  • Perttu Haimi
    • 1
  • Dalia Gelvonauskiene
    • 1
  • Vidmantas Stanys
    • 1
  1. 1.Insitute of Horticulture, Lithuanian Research Centre for Agriculture and ForestryKaunas distrLithuania

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