Differential expressions of photosynthetic genes provide clues to the resistance mechanism during Fusarium oxysporum f.sp. ciceri race 1 (Foc1) infection in chickpea (Cicer arietinum L.)
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Fusarium oxysporum f.sp. ciceri race 1 (Foc1), a root-invading pathogen causes vascular wilt in chickpea (Cicer arietinum L.). Foc1 is known to induce reactive oxygen species (ROS) mediated localized defense responses at the site of colonization in roots. However, the effect of this localized infection on distant shoot tissues is still unknown. In the present study, the effect of Foc1 on shoot tissues of both susceptible and resistant chickpea plants was studied. Total pigment content and fluorescence of chlorophyll was measured. Occurrence of oxidative damage in shoots was confirmed by both biochemical and lipid peroxidation assays. Expression pattern of some redox responsive transcripts were also analyzed. Additionally, transcriptional accumulations of some key genes related to light reaction, carbon reduction and photosystem II (PSII) of photosynthesis were analyzed at different time points post infection. Expressional status of stress induced sugar metabolism related genes (sucrose synthase, β amylase and invertase) were also investigated. Finally, gene networks were constructed showing interconnection of the photosynthetic genes, sugar metabolism-related genes and redox responsive transcripts with other metabolic and stress related pathways. The results demonstrate that the infection in root tissues of chickpea by Foc1 dramatically increases the ROS levels in shoot tissues of susceptible plants. The oxidative outburst in shoot tissues of susceptible plants also hampers the photosynthetic stability by down-regulating the key photosynthetic genes. On the contrary, resistant chickpea lines are grossly devoid of such instances with few behavioral irregularities at later time points.
KeywordsBiotic stress Cicer arietinum Fusarium oxysporum f.sp. ciceri race 1 Oxidative burst Photosynthetic genes Wilt disease
This work was supported by the grant provided to A. Bhar by Council of Scientific and Industrial Research, India (09/015(0378) /2009-EMR-1), to S. Gupta by Department of Biotechnology, Government of India (BT/PR9593/AGR/02/444/ 2007), to M. Chatterjee by Department of Biotechnology, Government of India (BT/01/COE/06/03/2006) and S. Sen by Indian Council of Agricultural Research (NFBSFARA/PB-2010/2010-11). S.Das was supported by funds provided by Bose Institute, Department of Science and Technology, Government of India. The funding organizations had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Authors thank Dr. S.C. Pande (ICRISAT, Patancheru) for providing fungal culture and Dr. S.K. Chaturvedi (IIPR, Kanpur) for providing chickpea seeds. Patient assistance of Mr. Swarnava Das for chlorophyll isolation and microscopic experiments is greatly acknowledged. Special thanks are reserved for Mr. Arup Kumar Dey for his help in green house experiments. Mr. Sudipta Basu is duly acknowledged for plant maintenance. Finally, authors acknowledge the Director, Bose Institute for infrastructural facilities.
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