Metabolic responses of avocado plants to stress induced by Rosellinia necatrix analysed by fluorescence and thermal imaging
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One of the most important soilborne diseases affecting avocado (Persea americana Mill.) crops is white root rot, caused by the fungus Rosellinia necatrix. In this study we investigated the metabolic responses elicited by white root rot in the aerial part of the plant with special focus on the potential applications of imaging technique (including chlorophyll fluorescence (Chl-F), blue-green fluorescence and thermography) in early detection of the disease on leaves. The results show that leaf metabolism was significantly affected by the infection only when symptoms started to appear, which was probably related to the loss of root functionality. However, changes in some Chl-F parameters provided early indications of stress even prior to the development of symptoms. We suggest that the combinatorial analysis of several Chl-F parameters could be used as a method for early detection of stress related to white root rot, and might prove useful as a general indicator of biotic and abiotic stress in avocado plants.
KeywordsRosellinia necatrix White root rot Avocado Fluorescence imaging Thermal imaging Plant stress
This work was supported by grants from CICE-Junta de Andalucía (Proyectos de Excelencia P08-CVI-03475, P10-AGR-5797 and P12-AGR-0370), Plan Nacional de I + D + I del Ministerio de Ciencia e Innovación, Spain (AGL2011-30354C0201) cofinanced by FEDER, EU and RECUPERA 2020/20134R060 (Ministerio de Economía y Competitividad-CSIC, Feder funds). E. Granum was recipient of a JAE-Doc contract funded by CSIC, Spain. C. E. Calderón was supported by a grant from FPI, Ministerio de Ciencia e Innovación, Spain.
Compliance with Ethical Standards
The Authors declare that the present manuscript complies with the Ethical Rules of good scientific practice applicable for the European Journal of Plant Pathology.
Conflict of Interest
The authors declare that they have no conflict of interest.
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