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European Journal of Plant Pathology

, Volume 142, Issue 3, pp 625–632 | Cite as

Metabolic responses of avocado plants to stress induced by Rosellinia necatrix analysed by fluorescence and thermal imaging

  • Espen Granum
  • María Luisa Pérez-Bueno
  • Claudia E. Calderón
  • Cayo Ramos
  • Antonio de Vicente
  • Francisco M. Cazorla
  • Matilde Barón
Article

Abstract

One of the most important soilborne diseases affecting avocado (Persea americana Mill.) crops is white root rot, caused by the fungus Rosellinia necatrix. In this study we investigated the metabolic responses elicited by white root rot in the aerial part of the plant with special focus on the potential applications of imaging technique (including chlorophyll fluorescence (Chl-F), blue-green fluorescence and thermography) in early detection of the disease on leaves. The results show that leaf metabolism was significantly affected by the infection only when symptoms started to appear, which was probably related to the loss of root functionality. However, changes in some Chl-F parameters provided early indications of stress even prior to the development of symptoms. We suggest that the combinatorial analysis of several Chl-F parameters could be used as a method for early detection of stress related to white root rot, and might prove useful as a general indicator of biotic and abiotic stress in avocado plants.

Keywords

Rosellinia necatrix White root rot Avocado Fluorescence imaging Thermal imaging Plant stress 

Notes

Acknowledgments

This work was supported by grants from CICE-Junta de Andalucía (Proyectos de Excelencia P08-CVI-03475, P10-AGR-5797 and P12-AGR-0370), Plan Nacional de I + D + I del Ministerio de Ciencia e Innovación, Spain (AGL2011-30354C0201) cofinanced by FEDER, EU and RECUPERA 2020/20134R060 (Ministerio de Economía y Competitividad-CSIC, Feder funds). E. Granum was recipient of a JAE-Doc contract funded by CSIC, Spain. C. E. Calderón was supported by a grant from FPI, Ministerio de Ciencia e Innovación, Spain.

Compliance with Ethical Standards

The Authors declare that the present manuscript complies with the Ethical Rules of good scientific practice applicable for the European Journal of Plant Pathology.

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

10658_2015_640_Fig1_ESM.gif (68 kb)
Suppl. Fig 1

Average values of Chl-F parameters FM/F0 (a), F’M/F’0 (b), F’V/F’M (c) and F’V/F’0 (d) in leaves of R. necatrix-infected avocado plants (R) and non-infected control plants (C) throughout the infection; means ± sd, n = 5. (GIF 68 kb)

10658_2015_640_MOESM1_ESM.tif (270 kb)
High resolution image (TIFF 270 kb)

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2015

Authors and Affiliations

  • Espen Granum
    • 1
  • María Luisa Pérez-Bueno
    • 1
  • Claudia E. Calderón
    • 2
  • Cayo Ramos
    • 3
  • Antonio de Vicente
    • 2
  • Francisco M. Cazorla
    • 2
  • Matilde Barón
    • 1
  1. 1.Departamento de Bioquímica, Biología Celular y Molecular de PlantasEstación Experimental del Zaidín. Consejo Superior de Investigaciones CientíficasGranadaSpain
  2. 2.Departamento de Microbiología, Facultad de CienciasInstituto de Hortofruticultura Subtropical y Mediterránea La Mayora. Universidad de Málaga, Consejo Superior de Investigaciones CientíficasMálagaSpain
  3. 3.Área de Genética, Facultad de CienciasInstituto de Hortofruticultura Subtropical y Mediterránea La Mayora. Universidad de Málaga, Consejo Superior de Investigaciones CientíficasMálagaSpain

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