Advertisement

European Journal of Plant Pathology

, Volume 141, Issue 3, pp 477–489 | Cite as

Identification and pathogenicity of Lasiodiplodia theobromae causing dieback of table grapes in Peru

  • E. Rodríguez-Gálvez
  • E. Maldonado
  • A. Alves
Original Research

Abstract

Production of table grapes is a major export commodity in the region of Piura, one of the most important grape-production areas of Peru. Characteristic symptoms of Botryosphaeria dieback were observed in vineyards throughout this region. The aim of this study was to characterise the symptoms of dieback of table grapes; evaluate the incidence and prevalence of the disease; identify the aetiological agent and evaluate its pathogenicity and virulence. The disease was present in all vineyards surveyed but incidence varied with location. A botryosphaeriaceous fungus was isolated from symptomatic plants collected in five different vineyards in Piura. On the basis of morphological and cultural characters as well as phylogenetic analysis of the internal transcribed spacer region (ITS), and part of the translation elongation factor 1-α gene (EF1-α) the isolates were identified as belonging to the species Lasiodiplodia theobromae. Inoculation of cv. Red Globe plants confirmed the pathogenicity of the isolates and revealed differences in virulence among the isolates tested. This is the first report of L. theobromae as causal agent of dieback of table grapes in Peru, which represents a threat to Peruvian vineyards productivity and longevity.

Keywords

Grapevine trunk diseases Lasiodiplodia Vitis vinifera Canker Dieback 

Notes

Acknowledgments

This work was partially financed by Instituto de Investigación para el Desarrollo (IIPD) de la Universidad Nacional de Piura, through the FEDU (fondo de investigación universitaria), by European Funds through COMPETE and by National Funds through the Portuguese Foundation for Science and Technology (FCT) within projects PTDC/AGR-FOR/3807/2012 - FCOMP-01-0124-FEDER-027979 and PEst-C/MAR/LA0017/2013. We thank two anonymous reviewers for some valuable comments on a draft of this paper.

References

  1. Akgul, D. S., Savas, N. G., & Eskalen, A. (2014). First report of wood canker caused by Botryosphaeria dothidea, Diplodia seriata, Neofusicoccum parvum, and Lasiodiplodia theobromae on grapevine in Turkey. Plant Disease, 98(4), 568.CrossRefGoogle Scholar
  2. Alama, I., Maldonado, E., & Rodríguez Gálvez, E. (2006). Lasiodiplodia theobromae afectando el Cultivo de Palto (Persea americana) en las condiciones de Piura-Perú. Universalia, 11(2), 4–13.Google Scholar
  3. Al-Saadoon, A. H., Ameen, M. K. M., Hameed, M. A., Al-Badran, A., & Ali, Z. (2012). First report of grapevine dieback caused by Lasiodiplodia theobromae and Neoscytalidium dimidiatum in Basrah, Southern Iraq. African Journal of Biotechnology, 11(95), 16165–16171.Google Scholar
  4. Alves, A., Correia, A., Luque, J., & Phillips, A. J. L. (2004). Botryosphaeria corticola sp. nov. on Quercus species, with notes and description of Botryosphaeria stevensii and its anamorph Diplodia mutila. Mycologia, 96, 598–613.PubMedCrossRefGoogle Scholar
  5. Alves, A., Phillips, A. J. L., Henriques, I., & Correia, A. (2007). Rapid differentiation of species of Botryosphaeriaceae by PCR fingerprinting. Research in Microbiology, 158, 112–121.PubMedCrossRefGoogle Scholar
  6. Alves, A., Crous, P. W., Correia, A., & Phillips, A. J. L. (2008). Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity, 28, 1–13.Google Scholar
  7. Amponsah, N. T., Jones, E. E., Ridgway, H. J., & Jaspers, M. V. (2011). Identification, potential inoculum sources and pathogenicity of botryosphaeriaceous species associated with grapevine dieback disease in New Zealand. European Journal of Plant Pathology, 131, 467–482.CrossRefGoogle Scholar
  8. Aroca, A., Raposo, R., Gramaje, D., Armengol, J., Martos, S. & Luque, J. (2008). First report of Lasiodiplodia theobromae associated with decline of grapevine rootstock mother plants in Spain. Plant Disease, 92(5), 832.Google Scholar
  9. Baskarathevan, J., Jaspers, M. V., Jones, E. E., & Ridgway, H. J. (2012). Identification, incidence and distribution of botryosphaeriaceous species in New Zealand vineyards. European Journal of Plant Pathology, 132, 549–560.CrossRefGoogle Scholar
  10. Bertsch, C., Ramírez-Suero, M., Magnin-Robert, M., Larignon, P., Chong, J., Abou-Mansour, E., Spagnolo, A., Clément, C., & Fontaine, F. (2013). Grapevine trunk diseases: complex and still poorly understood. Plant Pathology, 62(2), 243–265.CrossRefGoogle Scholar
  11. Burruano, S., Mondello, V., Conigliaro, G., Alfonzo, A., Spagnolo, A., & Mugnai, L. (2008). Grapevine decline in Italy caused by Lasiodiplodia theobromae. Phytopathologia Mediterranea, 47, 132–136.Google Scholar
  12. Correia, K. C., Câmara, M. P. S., Barbosa, M. A. G., Sales, R., Jr., Agustí-Brisach, C., Gramaje, D., León, M., García-Jiménez, J., Abad-Campos, P., Armengol, J., & Michereff, S. J. (2013). Fungal trunk pathogens associated with table grape decline in Northeastern Brazil. Phytopathologia Mediterranea, 52(2), 380–387.Google Scholar
  13. Damm, U., Crous, P. W., & Fourie, P. H. (2007). Botryosphaeriaceae as potential pathogens of Prunus species in South Africa, with descriptions of Diplodia africana and Lasiodiplodia plurivora sp. nov. Mycologia, 99, 664–680.PubMedCrossRefGoogle Scholar
  14. El-Goorani, M. A., & El Meleigi, M. A. (1972). Dieback of grapevine by Botryodiplodia theobromae Pat. in Egypt. Phytophatologia Mediterranean, 11, 210–211.Google Scholar
  15. Icochea, T., Perez, W., & Torres, H. (1995). First report of black rot of Oxalis tuberosa tubers caused by Lasiodiplodia theobromae. Plant Disease, 79, 425.CrossRefGoogle Scholar
  16. Kaiser, W. J., Rivero, V. G. M., & Valverde, B. E. (2009). First report of diplodia cane dieback of grapevine in Bolivia. Plant Disease, 93(3), 320.CrossRefGoogle Scholar
  17. Luque, J., Martos, S., Aroca, A., Raposo, R., & Garcia-Figueres, F. (2009). Symptoms and fungi associated with declining mature grapevine plants in northeast Spain. Journal of Plant Pathology, 91, 381–390.Google Scholar
  18. Martin, M. T., Martin, L., de Francisco, M. T., & Cobos, R. (2009). First report of Lasidiplodia theobromae and Cryptovalsa ampelina associated with grapevine decline from Castilla y León, Spain. Plant Disease, 93(5), 545.CrossRefGoogle Scholar
  19. Netto, M. S. B., Assunção, I. P., Lima, G. S. A., Marques, M. W., Lima, W. G., Monteiro, J. H. A., Balbino, V. Q., Michereff, S. J., Phillips, A. J. L. & Câmara, M. P. S. (2014). Species of Lasiodiplodia associated with papaya stem-end rot in Brazil. Fungal Diversity.Google Scholar
  20. Page, R. D. (1996). TreeView: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences, 12, 357–358.PubMedGoogle Scholar
  21. Phillips, A. J. L. (2002). Botryosphaeria species associated with diseases of grapevines in Portugal. Phytopathologia Mediterranea, 41, 3–18.Google Scholar
  22. Phillips, A. J. L., Crous, P. W., & Alves, A. (2007). Diplodia seriata, the anamorph of “Botryosphaeria” obtusa. Fungal Diversity, 25, 141–155.Google Scholar
  23. Phillips, A. J. L., Alves, A., Abdollahzadeh, J., Slippers, B., Wingfield, M. J., Groenewald, J. Z., & Crous, P. W. (2013). The Botryosphaeriaceae: Genera and species known from culture. Studies in Mycology, 76, 51–167.PubMedCentralPubMedCrossRefGoogle Scholar
  24. Pitt, W. M., Huang, R., Steel, C. C., & Savocchia, S. (2013). Pathogenicity and epidemiology of Botryosphaeriaceae species isolated from grapevines in Australia. Australasian Plant Pathology, 42(5), 573–582.CrossRefGoogle Scholar
  25. Punithalingam, E. (1980). Plant diseases attributed to Botryodiplodia theobromae. In: Biblioteca Mycologica. J. Cramer, Berlin.Google Scholar
  26. Qiu, Y., Steel, C. C., Ash, G. J., & Savocchia, S. (2011). Survey of Botryosphaeriaceae associated with grapevine decline in the hunter valley and mudgee grape growing regions of New South Wales. Australasian Plant Pathology, 40, 1–11.CrossRefGoogle Scholar
  27. Rodriguez, F., Oliver, J. F., Marin, A., & Medina, J. R. (1990). The general stochastic model of nucleotide substitutions. Journal of Theoretical Biology, 142, 485–501.PubMedCrossRefGoogle Scholar
  28. Rodríguez-Gálvez, E., Maldonado, D. E., & Díaz, M. A. (1999). Incidencia de la muerte apical descendente del mango causada por Lasiodiplodia theobromae en Piura, Peru. Fitopatología, 34(2), 90–95.Google Scholar
  29. Savocchia, S., Steel, C. C., Stodard, B. J., & Somers, A. (2007). Pathogenicity of Botryosphaeria species isolated from declining grapevines in sub tropical regions of Eastern Australia. Vitis, 46, 27–32.Google Scholar
  30. Swofford, D. L. (2003). PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4.0. Sunderland: Sinauer Associates.Google Scholar
  31. Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.PubMedCentralPubMedCrossRefGoogle Scholar
  32. Taylor, A., Hardy, G. E., St, J., Wood, P., & Burgess, T. (2005). Identification and pathogenicity of Botryosphaeria species associated with grapevine decline in Western Australia. Australasian Plant Pathology, 34, 187–195.CrossRefGoogle Scholar
  33. Teng, P. S. and James, W. C. 2001.Disease and yield loss assessment. In: Waller, J.M., Lenné, J.M. and Waller, S.J. Plant Pathologist’s pocketbook. CABI Publishing. Wallingford. Oxon, UK.516 p.Google Scholar
  34. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., & Higgins, D. G. (1997). The ClustalX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882.PubMedCentralPubMedCrossRefGoogle Scholar
  35. Úrbez-Torres, J. R. (2011). The status of Botryosphaeriaceae species infecting grapevines. Phytopathologia Mediterranea, 50(Suppl), 5–45.Google Scholar
  36. Úrbez-Torres, J. R., & Gubler, W. D. (2009). Pathogenicity of Botryosphaeriaceae species isolated from grapevine cankers in California. Plant Disease, 93, 584–592.CrossRefGoogle Scholar
  37. Úrbez-Torres, J. R., Leavitt, G. M., Voegel, T. M., & Gubler, W. D. (2006). Identification and distribution of Botryosphaeria spp. associated with grapevine cankers in California. Plant Disease, 90, 1490–1503.CrossRefGoogle Scholar
  38. Úrbez-Torres, J. R., Leavitt, G. M., Guerrero, J. C., Guevara, J., & Gubler, W. D. (2008). Identification and pathogenicity of Lasiodiplodia theobromae and Diplodia seriata, the causal agents of bot canker disease of grapevines in Mexico. Plant Disease, 92, 519–529.CrossRefGoogle Scholar
  39. Úrbez-Torres, J. R., Peduto, F., & Gubler, W. D. (2010). First report of grapevine cankers caused by Lasiodiplodia crassispora and Neofusicoccum mediterraneum in California. Plant Disease, 94(6), 785.Google Scholar
  40. Úrbez-Torres, J. R., Peduto, F., Striegler, R. K., Urrea-Romero, K. E., Rupe, J. C., Cartwright, R. D., & Gubler, W. D. (2012). Characterization of fungal pathogens associated with grapevine trunk diseases in Arkansas and Missouri. Fungal Diversity, 52(1), 169–189.CrossRefGoogle Scholar
  41. van Niekerk, J. M., Crous, P. W., Groenewald, J. Z., Fourie, P. H., & Halleen, F. (2004). DNA phylogeny, morphology and pathogenicity of Botryosphaeria species on grapevines. Mycologia, 96, 781–798.PubMedCrossRefGoogle Scholar
  42. van Niekerk, J. M., Fourie, P. H., Halleen, F., & Crous, P. W. (2006). Botryosphaeria spp. as grapevine trunk disease pathogens. Phytopathologia Mediterranea, 45, S43–S54.Google Scholar
  43. van Niekerk, J. M., Bester, W., Halleen, F., Crous, P. W., & Fourie, P. H. (2010). First report of Lasiodiplodia crassispora as a pathogen of grapevine trunks in South Africa. Plant Disease, 94(8), 1063.CrossRefGoogle Scholar
  44. Vivier, M. A., & Pretorius, I. S. (2002). Genetically tailored grapevines for the wine industry. Trends in Biotechnology, 20, 472–478.PubMedCrossRefGoogle Scholar
  45. Webb, R. & Fernández B.G. (2013). Anuario Estadístico El Perú en Números. Ed. Tarea Asociación Gráfica Educativa. Lima, Perú. 1271 pp.Google Scholar
  46. Yan, J.-Y., Xie, Y., Zhang, W., Wang, Y., Liu, J.-K., Hyde, K. D., Seem, R. C., Zhang, G.-Z., Wang, Z.-Y., Yao, S.-W., Bai, X.-J., Dissanayake, A. J., Peng, Y.-L., & Li, X.-H. (2013). Species of Botryosphaeriaceae involved in grapevine dieback in China. Fungal Diversity, 61(1), 221–236.CrossRefGoogle Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2014

Authors and Affiliations

  1. 1.Departamento de Sanidad Vegetal, Facultad de AgronomíaUniversidad Nacional de PiuraPiuraPeru
  2. 2.Departamento de Biologia, CESAMUniversidade de AveiroAveiroPortugal

Personalised recommendations