Advertisement

European Journal of Plant Pathology

, Volume 141, Issue 3, pp 611–615 | Cite as

Complete genomic sequence of a Pineapple mealybug wilt-associated virus-1 from Hainan Island, China

  • Naitong Yu
  • Zhiwen Luo
  • Hongyan Fan
  • Zhili Zhang
  • Xianghong Li
  • Jianhua Wang
  • Zhixin LiuEmail author
  • Fan HeEmail author
Article

Abstract

This is the first report of the complete genome sequence of Pineapple mealybug wilt-associated virus-1 (PMWaV-1 HN, KJ872494) identified on pineapple [Ananas comosus (L.) Merr.] in Hainan island, China. The genome is 13,069 nucleotides long, possessing seven open reading frames (ORFs). The ORF1 encodes a 233 kDa polyprotein which has PRO protease domain, MTR methyltransferase domain and HEL helicase domain; The ORF2 ~ ORF7 encode RNA-dependent RNA polymerase protein (RdRp), a 6 kDa protein (p6), heat shock 70 homolog protein (HSP70h), a 46 kDa protein (p46), coat protein (CP) and a 24 kDa protein (p24), respectively. Full-length nucleotide sequence analysis of PMWaV-1 HN genome confirmed it 97.99 % homology with PMWaV-1 (AF414119) which was isolated from Hawaii, USA. Furthermore, based on Maximum Likelihood method, all conserved trees of RdRp, HSP70h and CP proteins sequences revealed that PMWaV-1 HN together with other PMWaV-1 isolates clearly separated into PMWaV-1 group.

Keywords

Genome Pineapple mealybug wilt-associated virus-1 Sequencing 

Abbreviations

PMWaV-1

Pineapple mealybug wilt-associated virus-1

PMWaV-2

Pineapple mealybug wilt-associated virus-2

PMWaV-3

Pineapple mealybug wilt-associated virus-3

MWP

Mealybug wilt of pineapple

Notes

Acknowledgments

This work was supported by the Natural Science Fund of Hainan Province (NO.313096), the Scientific Research Fund of Hainan Province (KYYS-2013-02) and the Major Science and Technology Program of Hainan Province (ZDZX2013023-1).

Supplementary material

10658_2014_545_MOESM1_ESM.doc (26 kb)
Fig. S1 (DOC 26 kb)
10658_2014_545_MOESM2_ESM.doc (26 kb)
Fig. S2 (DOC 26 kb)
10658_2014_545_MOESM3_ESM.doc (26 kb)
Fig. S3 (DOC 26 kb)

References

  1. Gambley, C. F., Steele, V., Geering, A. D. W., & Thomas, J. E. (2008). The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease. Australasian Plant Pathology, 37, 95–105.CrossRefGoogle Scholar
  2. Hughes, G., & Samita, S. (1998). Analysis of patterns of pineapple mealybug wilt disease in Sri Lanka. Plant Disease, 82, 885–890.CrossRefGoogle Scholar
  3. King, E., Lefkowitz, E., Adams, M., & Carstens, E. (2011). Virus taxonomy: Ninth report of the international committee on taxonomy of viruses. London: Elsevier.Google Scholar
  4. Li, Y. H., Mo, Y. W., Xi, J. G., Zhang, L. B., & Sun, G. M. (2010). Detection of pineapple mealybug wilt-associated viruses with RT-PCR. Chinese journal of tropical crops, 31, 1003–1008.Google Scholar
  5. Melzer, M. J., Karasev, A. V., Sether, D. M., & Hu, J. S. (2001). Nucleotide sequence, genome organization and phylogenetic analysis of pineapple mealybug wilt-associated virus-2. Journal of General Virology, 82, 1–7.PubMedGoogle Scholar
  6. Melzer, M. J., Sether, D. M., Karasev, A. V., Borth, W., & Hu, J. S. (2008). Complete nucleotide sequence and genome organization of pineapple mealybug wilt-associated virus-1. Archives of Virology, 153, 707–714.PubMedCrossRefGoogle Scholar
  7. Peremyslov, V. V., Hagiwara, Y., & Dolja, V. V. (1999). HSP70 homolog functions in cell-to-cell movement of a plant virus. Proceedings of the National Academy of Sciences, 96, 14771–14776.CrossRefGoogle Scholar
  8. Sether, D. M., Melzer, M. J., Borth, W. B., & Hu, J. S. (2009). Genome organization and phylogenetic relationship of Pineapple mealybug wilt associated virus-3 with family Closteroviridae members. Virus Genes, 38, 414–420.PubMedCrossRefGoogle Scholar
  9. Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.PubMedCentralPubMedCrossRefGoogle Scholar
  10. Ullman, D. E., German, T. L., Gunasinghe, U. B., & Ebesu, R. H. (1989). Serology of a closteroviruslike particle associated with mealybug wilt of pineapple. Phytopathology, 79, 1341–1345.CrossRefGoogle Scholar
  11. Xu, C. M. (2009). Advances on researches of pineapple mealybug wilt associated viruses. Chinese journal of tropical crops, 30, 718–724.Google Scholar

Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2014

Authors and Affiliations

  • Naitong Yu
    • 1
    • 2
  • Zhiwen Luo
    • 1
  • Hongyan Fan
    • 1
  • Zhili Zhang
    • 1
  • Xianghong Li
    • 1
  • Jianhua Wang
    • 2
  • Zhixin Liu
    • 2
    Email author
  • Fan He
    • 1
    Email author
  1. 1.Institute of Tropical Fruit Tree, Hainan Academy of Agricultural Science/Haikou Investigation Station of Tropical Fruit Trees, Ministry of AgricultureHaikouChina
  2. 2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikouChina

Personalised recommendations