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European Journal of Plant Pathology

, Volume 139, Issue 3, pp 511–524 | Cite as

Chlorocholine chloride mediated resistance mechanism and protection against leaf spot disease of Stevia rebaudiana Bertoni

  • Sayanti Kundu
  • Avishek Dey
  • Abhijit BandyopadhyayEmail author
Article
  • 266 Downloads

Abstract

Investigations have been carried out to determine the influence of chlorocholine chloride on induction of the resistance mechanisms of Stevia rebaudiana against leaf spot disease, caused by Alternaria alternata. The paper also focuses an impact of chlorocholine chloride induced resistance on reduction of leaf spot disease. Chlorocholine chloride is attributed to its significant role in defence responses through augmentation of phenol and salicylic acid content as well as stimulation of phenylalanine ammonia lyase and peroxidase activity in S. rebaudiana following inoculation with A. alternata. Histochemical studies revealed that fungal invasion as well as infection process was appreciably reduced in chlorocholine chloride treated plants through peroxidase-H2O2 mediated strengthening of cell wall. The overall study highlights the significant role of chlorocholine chloride in induction of resistance in S. rebaudiana against A. alternata.

Keywords

Stevia rebaudiana Alternaria alternata Leaf spot disease Chlorocholine chloride Resistance 

Abbreviations

ANOVA

Analysis of variance

CCC

Chlorocholine chloride

EDTA

Ethylene diamine tetra acetate

g

Gram

h

Hour

MS

Murashige & Skoog

mg

Milligram

mg/l

Milligram per litre

min

Minute

ml

Milliliter

μg

Microgram

μl

Microlitre

μm

micrometer

nm

Nanometer

PAL

Phenylalanine ammonia lyase

PAGE

Polyacrylamide gel electrophoresis

PDA

Potato dextrose agar

ROS

Reactive oxygen species

rpm

Rotation per minute

SA

Salicylic acid

TEMED

Tetramethylethylenediamine

UV

Ultra violet

w/v

Weight by volume

v/v

Volume by volume

Notes

Acknowledgments

Authors gratefully acknowledge their respected supervisor for valuable and constructive suggestions. We are also grateful to Prof. N. C. Mondal, Visva-Bharati University, West Bengal, India, for providing pure culture of the pathogen. We are thankful to the Head, Department of Botany, The University of Burdwan for providing laboratory facilities. Financial assistance from Government of West Bengal, India is duly acknowledged.

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Copyright information

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2014

Authors and Affiliations

  • Sayanti Kundu
    • 1
  • Avishek Dey
    • 1
  • Abhijit Bandyopadhyay
    • 1
    Email author
  1. 1.UGC Centre for Advanced Study, Department of BotanyThe University of BurdwanBurdwanIndia

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