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European Journal of Plant Pathology

, Volume 138, Issue 1, pp 93–101 | Cite as

Characterization of Potato spindle tuber viroid (PSTVd) incidence and new variants from ornamentals

  • Jaroslav Matoušek
  • Rajen J. J. Piernikarczyk
  • Petr Dědič
  • Josef Mertelík
  • Kateřina Uhlířová
  • Ganesh S. Duraisamy
  • Lidmila Orctová
  • Kateřina Kloudová
  • Jiří Ptáček
  • Gerhard Steger
Article

Abstract

We analyzed the spreading and persistence of PSTVd variants in several ornamentals in the territory of the Czech Republic. The pool of PSTVd variants detected in Solanum jasminoides, S. muricatum, Datura sp. and Brugmansia sp. was biolistically transferred to Matricaria chamomilla, Argyranthemum frutescens and Diascia sp., species which we found as sensitive hosts for PSTVd from ornamentals. The PSTVd pool showed sequence changes and increased variation after its transfer to potato, suggesting a wide adaptation potential of PSTVd in this crop. Potato exhibited genotype-dependent leaf and spindle tuber symptoms, when inoculated with the sap from S. jasminoides infected with the predominant and sequence-stable PSTVd-S1.

Keywords

Viroid pathogenicity Real-time PCR Biolistic inoculation of plants Solanum jasminoides Solanum tuberosum Solanum lycopersicum 

Notes

Acknowledgments

The authors would like to acknowledge help and fruitful discussions with Dr. Csaba Koncz (Max-Planck-Institut für Züchtungsforschung, Cologne, Germany). The authors thank Mrs. Helena Matoušková, Ing. Olga Horáková and bc. Martin Selinger (BC v.v.i. IPMB, České Budějovice), Bernd Esters (Institute of Physical Biology, Heinrich-Heine-Universität Düsseldorf, Germany) for their help and excellent technical assistance. The project was supported by the Czech Science Foundation (GACR P501/10/J018), by the cooperative project FP7-REGPOT-2012-2013-1 MODBIOLIN No. 316304, by the project QH81262 (NAZV CR), and by institutional support (RVO:60077344) to J.M.

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Copyright information

© KNPV 2013

Authors and Affiliations

  • Jaroslav Matoušek
    • 1
  • Rajen J. J. Piernikarczyk
    • 4
  • Petr Dědič
    • 2
  • Josef Mertelík
    • 3
  • Kateřina Uhlířová
    • 1
  • Ganesh S. Duraisamy
    • 1
  • Lidmila Orctová
    • 1
  • Kateřina Kloudová
    • 3
  • Jiří Ptáček
    • 2
  • Gerhard Steger
    • 4
  1. 1.BC ASCR v.v.i., Institute of Plant Molecular BiologyČeské BudějoviceCzech Republic
  2. 2.Potato Research InstituteHavlíčkův BrodCzech Republic
  3. 3.Silva Tarouca Research Institute for Landscape and Ornamental GardeningPrůhoniceCzech Republic
  4. 4.Institute of Physical BiologyHeinrich-Heine-Universität DüsseldorfDüsseldorfGermany

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