Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates
Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008–09. Mixed infections were the most frequent type in 2007–08 in contrast to 2009 when single infection of ZYMV prevailed. ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo ‘Beogradska’) squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide.
KeywordsCucurbit viruses RT-PCR Sequencing Serological identification Virus incidence
This study was supported by the Ministry for Education and Science of the Republic of Serbia (Project Nos. III-43001 and TR-31025).
- Dukić, N., Krstić, B., Vico, I., Berenji, J., & Duduk, B. (2006). First report of Zucchini yellow mosaic virus, Watermelon mosaic virus and Cucumber mosaic virus in bottlegourd (Lagenaria siceraria) in Serbia. Plant Disease, 90, 380.Google Scholar
- FAO (2008). The FAO Statistical Database (FAOSTAT): Food and Agriculture Organization of the United Nations. Retrieved from http://faostat.fao.org.
- Iwaki, M., Honda, Y., Hanada, K., Tochihara, H., Yonaha, T., Hokama, K., & Yokoyama, T. (1984). Silver mottle disease of watermelon caused by tomato spotted wilt virus. Plant Disease, 68, 1006–1008.Google Scholar
- Lecoq, H., Wisler, G., & Pitrat, M. (1998). Cucurbit viruses: The classics and the emerging. In J. D. McCreight (Ed.), Cucurbitaceae ’98: Evaluation and enhancement of cucurbit germplasm (pp. 126–142). Alexandria: ASHS.Google Scholar
- Marchoux, G., Hostachy, B., Gebre-Selassie, K., & Gognalons, P. (2000). Tomato spotted wilt virus: hôtes et méthodes de lutte. PHM—Revue Horticole, 418, 46–52.Google Scholar
- Nagata, T., de Ávila, A. C., Tavares, P. C. M., Barbosa, C. J., Juliatti, F. C., & Kitajima, E. W. (1995). Occurrence of different tospoviruses in six states of Brazil. Fitopatologia Brasiliera, 20, 90–95.Google Scholar
- Pfosser, M. F., & Baumann, H. (2002). Phylogeny and geographical differentiation of zucchini yellow mosaic virus isolates (Potyviridae) based on molecular analysis of the coat protein and part of the cytoplasmic inclusion protein genes. Archives of Virology, 147, 1599–1609.PubMedCrossRefGoogle Scholar
- Provvidenti, R. (1996). Diseases caused by viruses. In T. A. Zitter, D. L. Hopkins, & C. E. Thomas (Eds.), Compendium of Cucurbit Diseases (pp. 37–45). St. Paul: American Phytopathological Society.Google Scholar
- Rao, X., Liu, Y., Wu, Z., & Li, Y. (2011). First report of natural infection of watermelon by Watermelon silver mottle virus in China. New Disease Reports, 24, 12.Google Scholar
- Silveira, W. G., Jr., Avila, A. C., & Muñoz, J. O. (1985). Chayote (Sechium edule Sw): a new host of tomato spotted wilt virus. Fitopatologia Brasileira, 10, 661–665.Google Scholar
- Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J., & Krstić, B. (2011). The current status of cucurbit viruses in Serbia (pp. 109). 4th Conference of the International Working Group on Legume and Vegetable Viruses (IWGLVV), Antequera, Spain.Google Scholar
- Wang, Y., Gaba, V., Yang, J., Palukaitis, P., & Gal-On, A. (2002). Characterizations of synergy between cucumber mosaic virus and potyviruses in cucurbit hosts. Phytopathology, 147, 2301–2312.Google Scholar
- Zeng, R., Liao, Q., Feng, J. D., Li, J. D., & Chen, J. (2007). Synergy between Cucumber mosaic virus and Zucchini yellow mosaic virus on Cucurbitaceae hosts tested by real-time reverse transcription-polymerase chain reaction. Acta Biochimica et Biophysica Sinica, 39, 431–437.PubMedCrossRefGoogle Scholar