European Journal of Plant Pathology

, Volume 136, Issue 2, pp 273–280 | Cite as

A new quantitative real-time PCR assay for Rhizoctonia solani AG3-PT and the detection of AGs of Rhizoctonia solani associated with potato in soil and tuber samples in Great Britain

  • James W. Woodhall
  • Ian P. Adams
  • Jeff C. Peters
  • Glyn Harper
  • Neil Boonham
Article

Abstract

Rhizoctonia solani is an important pathogen of potatoes causing stem canker and black scurf. The fungus is a species complex comprised of 13 known anastomosis groups (AGs). AG3-PT is the anastomosis group frequently associated with disease in potatoes. A real-time PCR assay was designed to the rDNA ITS region of AG3-PT isolates to enable the pathogen to be detected directly in tuber and soil samples. The resulting assay was highly specific for AG3-PT, and did not amplify DNA from isolates from other AGs or subgroups of AG3. Using a bulk DNA extraction method capable of extracting from up to 250 g of soil, the assay could detect one individual sclerotium of AG3-PT (weighing 200 μg) in 250 g of soil. The AG3-PT assay was used, with assays for AG2-1, AG5 and AG8 to determine the prevalence of those AGs in UK potato soils and tubers. AG2-1 and AG3-PT were the predominant groups in tubers and soils, although AG3-PT was more frequently isolated from tubers, highlighting its importance as a potato pathogen. AG3-PT was also detected in more than half of the tuber samples tested suggesting the importance of seed borne inoculum.

Keywords

Rhizoctonia solani AG3-PT Potatoes Real-time PCR Soil 

Abbreviations

AG

Anastomosis group

Ct

Cycle threshold

ITS

Internal transcribed spacer

PDA

Potato dextrose agar

rDNA

Ribosomal DNA

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Copyright information

© UK Crown Copyright 2013

Authors and Affiliations

  • James W. Woodhall
    • 1
  • Ian P. Adams
    • 1
  • Jeff C. Peters
    • 1
  • Glyn Harper
    • 2
  • Neil Boonham
    • 1
  1. 1.The Food and Environment Research AgencyYorkUK
  2. 2.AHDB Potato Council, Sutton Bridge Crop Storage ResearchSpaldingUK

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