European Journal of Plant Pathology

, Volume 136, Issue 2, pp 261–271

Sugar beet extract induces defence against Phytophthora infestans in potato plants

  • Laith Ibrahim Moushib
  • Johanna Witzell
  • Marit Lenman
  • Erland Liljeroth
  • Erik Andreasson
Article

Abstract

The aim of this study was to find a natural and cheap agent that could induce defence responses in potato plants to combat Phytophthora infestans, which causes late blight disease that is one of the most devastating plant pathogens in agriculture. We tested whether a sugar beet extract (SBE), derived through a simple extraction procedure from a large-scale plant waste product, induced resistance under green-house conditions. In three potato genotypes differing in their level of resistance to P. infestans (two susceptible genotypes: Desiree and Bintje and one partially resistant: Ovatio), treatment with SBE resulted in significant reduction of the size of the infection lesions in a pattern similar to that seen with application of a known defence-inducing compound, β-aminobutyric acid (BABA). Lower sporangial production was also observed on SBE-treated leaves, but the reduction in sporangial production was more pronounced after BABA treatment. SBE had no apparent toxic effect on the hyphal growth of the pathogen or on the germination of sporangia. Instead, SBE triggered pathogenesis-related protein (PR-1 and PR-2) induction which suggests that the protection conferred by SBE could be via induced resistance. An array of phenolic metabolites was found in the SBE that may contribute to the defence response.

Keywords

BABA Defence-inducing agent Induced resistance Plant extract Potato late blight PR-1 

Abbreviations

BABA

β-aminobutyric acid

Dpi

Days post inoculation

IPM

Integrated pest management

PAMP

Pathogen-associated molecular patterns

PR-1 protein

Pathogenesis-related protein 1

SBE

Sugar beet extract

SDS-PAGE

Sodium dodecyl sulphate polyacrylamide gel electrophoresis

References

  1. Beckers, G. J. M., & Conrath, U. (2007). Priming for stress resistance: from the lab to the field. Current Opinion in Plant Biology, 10, 425–431.PubMedCrossRefGoogle Scholar
  2. Boller, T., & Felix, G. (2009). A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annual Review of Plant Biology, 60, 379–406.PubMedCrossRefGoogle Scholar
  3. Boubakri, H., Wahab, M. A., Chong, J., Bertsch, C., Mliki, A., & Soustre-Gacougnolle, I. (2012). Thiamine induced resistance to Plasmopara viticola in grapevine and elicited host-defense responses, including HR like-cell death. Plant Physiology and Biochemistry, 57, 120–133.PubMedCrossRefGoogle Scholar
  4. Cohen, Y. (1994). Local and systemic control of Phytophthora infestans in tomato plants by DL-3-amino-n-butanoic acids. Phytopathology, 84, 55–59.CrossRefGoogle Scholar
  5. Cohen, Y. (2002). β-Aminobutyric acid induced resistance against plant pathogens. Plant Disease, 86, 448–457.CrossRefGoogle Scholar
  6. Curtis, H., Noll, U., Stormann, J., & Slusarenko, A. (2004). Broad-spectrum activity of the volatile phytoanticipin allicin in extracts of garlic (L.) against plant pathogenic bacteria, fungi and oomycetes. Physiological and Molecular Plant Pathology, 65, 79–89.CrossRefGoogle Scholar
  7. Deepak, S. A., Niranjan, R. S., Umemura, K., Kono, T., & Shetty, H. S. (2003). Cerebroside as an elicitor for induced resistance against the downy mildew pathogen in pearl millet. Annals of Applied Biology, 143, 169–173.CrossRefGoogle Scholar
  8. Devaiah, S. P., Mahadevappa, G. H., & Shetty, H. S. (2009). Induction of systemic resistance in pearl millet (Pennisetum glaucum) against downy mildew (Sclerospora graminicola) by Datura metel extract. Crop Protection, 28, 783–791.CrossRefGoogle Scholar
  9. Fought, L., & Kuć, J. A. (1996). Lack of specificity in plant extracts and chemicals as inducers of systemic resistance in cucumber plants to anthracnose. Journal of Phytopathology, 144, 1–6.CrossRefGoogle Scholar
  10. Fry, W. (2008). Phytophthora infestans: the plant (and R gene) destroyer. Molecular Plant Pathology, 9, 385–402.PubMedCrossRefGoogle Scholar
  11. Gust, A., Brunner, F., & Nürnberger, T. (2010). Biotechnological concepts for improving plant innate immunity. Current Opinion in Biotechnology, 21, 204–210.PubMedCrossRefGoogle Scholar
  12. Jakab, G., Ton, J., Flors, V., Zimmerli, L., Metraux, J. P., & Mauch-Mani, B. (2005). Enhancing Arabidopsis salt and drought stress tolerance by chemical priming for its abscisic acid responses. Plant Physiology, 139, 267–274.PubMedCrossRefGoogle Scholar
  13. Jeun, Y. C., Siegrist, J., & Buchnauer, H. (2000). Biochemical and cytological studies on mechanisms of systemically induced resistance to Phytophthora infestans in tomato plants. Journal of Phytopathology, 148, 129–140.Google Scholar
  14. Kashiwagi, K., Furuno, N., Kitamura, S., Ohta, S., Sugihara, K., Utsumi, K., Hanada, H., Taniguchi, K., Suzuki, K., & Kashiwagi, A. (2008). Disruption of thyroid hormone function by environmental pollutants. Journal of Health Science, 55, 147–160.CrossRefGoogle Scholar
  15. Kuć, J. (2001). Concepts and direction of induced systemic resistance in plants and its application. European Journal of Plant Pathology, 107, 7–12.Google Scholar
  16. Langer, P., Koćan, A., Tajtáková, M., Trnovec, T., & Klimes, I. (2009). What we learned from the study of exposed population to PCBs and pesticides. The Open Environmental Pollution and Toxicology Journal, 1, 54–65.CrossRefGoogle Scholar
  17. Liljeroth, E., Bengtsson, T., Wiik, L., & Andreasson, E. (2010). Induced resistance in potato to Phytphthora infestans - effects of BABA in greenhouse and field tests with different potato varieties. European Journal of Plant Pathology, 127, 171–183.CrossRefGoogle Scholar
  18. Martín, J. A., Solla, A., Witzell, J., Gil, L., & Garcia-Vallejo, M. C. (2010). Antifungal effect and reduction of Ulmus minor symptoms to Ophiostoma novo-ulmi by carvacrol and salicylic acid. European Journal of Plant Pathology, 127, 21–32.CrossRefGoogle Scholar
  19. Postel, S., & Kemmerling, B. (2009). Plant systems for recognition of pathogen-associated molecular patterns. Seminars in Cell & Developmental Biology, 20, 1025–1031.CrossRefGoogle Scholar
  20. Röhner, E., Carabet, A., & Buchenauer, H. (2004). Effectiveness of plant extracts of Paeonia suffruticosa and Hedera helix against diseases caused by Phytophthora infestans in tomato and Pseudoperonospora cubensis in cucumber. Journal of Plant Diseases and Protection, 111, 83–95.Google Scholar
  21. Sharma, K., Butz, A. F., & Finckh, M. R. (2010). Effects of host and pathogen genotypes on inducibility of resistance in tomato (Solanum lycopersicum) to Phytophthora infestans. Plant Pathology, 59, 1062–1071.CrossRefGoogle Scholar
  22. Slusarenko, A. J., Patel, A., & Portz, D. (2008). Control of plant diseases by natural products: Allicin from garlic as a case study. European Journal of Plant Pathology, 121, 313–322.CrossRefGoogle Scholar
  23. Soylu, E. M., Soylu, S., & Kurt, S. (2006). Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent Phytophthora infestans. Mycopathologia, 161, 119–128.PubMedCrossRefGoogle Scholar
  24. Stephan, D., Schmitt, A., Carvalho, M., Seddon, B., & Koch, E. (2005). Evaluation of biocontrol preparations and plant extracts for the control of Phytophthora infestans on potato leaves. European Journal of Plant Pathology, 112, 235–246.CrossRefGoogle Scholar
  25. Thuerig, B., Binder, A., Boller, T., Guyer, U., Jiménez, S., Rentsch, C., & Tamm, L. (2006). An aqueous extract of the dry mycelium of Penicillium chrysogenum induces resistance in several crops under controlled and field conditions. European Journal of Plant Pathology, 114, 185–197.CrossRefGoogle Scholar
  26. Unger, C., Wilhelm, I., Jünger, R., & Thalmann, R. (2006). Evidence of induced resistance of tomato plants against Phytophthora infestans by a water extract of dried biomass of Penicillium chrysogenum. Journal of Plant Diseases and Protection, 113, 1–9.Google Scholar
  27. Van Loon, L. C., Rep, M., & Pieterse, C. M. J. (2006). Significance of inducible defense-related proteins in infected plants. Annual Review of Phytopathology, 44, 135–162.PubMedCrossRefGoogle Scholar
  28. Walters, D. R., & Fountaine, J. M. (2009). Practical application of induced resistance to plant diseases: an appraisal of effectiveness under field conditions. The Journal of Agricultural Science, 147, 523–535.CrossRefGoogle Scholar
  29. Walters, D., Walsh, D., Newton, A., & Lyon, G. (2005). Induced resistance for plant disease control: maximizing the efficacy of resistance elicitors. Phytopathology, 95, 1368–1373.PubMedCrossRefGoogle Scholar
  30. Widmer, T. L., & Laurent, N. (2006). Plant extracts containing caffeic acid and rosmarinic acid inhibit zoospore germination of Phytophthora spp. pathogenic to Theobroma cacao. European Journal of Plant Pathology, 115, 377–388.CrossRefGoogle Scholar
  31. Wu, C. C., Singh, P., Chen, M. C., & Zimmerli, L. (2010). L-Glutamine inhibits beta-aminobutyric acid-induced stress resistance and priming in Arabidopsis. Journal of Experimental Botany, 61, 995–1002.PubMedCrossRefGoogle Scholar
  32. Yang, L., Zambrano, Y., Hu, C.-J., Carmona, E. R., Bernal, A., Pérez, A., Zayas, C. M., Li, Y.-R., Guerra, A., Santana, I., & Arencibia, A. D. (2010). Sugarcane metabolites produced in CO2-rich temporary immersion bioreactors (TIBs) induce tomato (Solanum lycopersicum) resistance against bacterial wilt (Ralstonia solanacearum). In vitro cellular and developmental biology-Plant, 46, 558–568.Google Scholar

Copyright information

© KNPV 2013

Authors and Affiliations

  • Laith Ibrahim Moushib
    • 1
  • Johanna Witzell
    • 2
  • Marit Lenman
    • 3
  • Erland Liljeroth
    • 3
  • Erik Andreasson
    • 3
  1. 1.Department of BiologyLund UniversityLundSweden
  2. 2.Department of Southern Swedish Forest Research CentreSwedish University of Agricultural SciencesAlnarpSweden
  3. 3.Department of Plant Protection BiologySwedish University of Agricultural SciencesAlnarpSweden

Personalised recommendations