European Journal of Plant Pathology

, Volume 135, Issue 4, pp 805–815

Diversity of sooty blotch and flyspeck fungi from apples in northeastern Turkey

  • Derrick A. Mayfield
  • Aziz Karakaya
  • Jean C. Batzer
  • Jennifer M. Blaser
  • Mark L. Gleason


Sooty blotch and flyspeck (SBFS) is a mid- to late-season fungal disease complex that blemishes apples and other tree fruit crops in humid regions worldwide. SBFS is a concern for apple growers in northeastern Turkey, but the composition of the SBFS species assemblage in Turkey is unknown. In this study, SBFS fungi were isolated from apples collected in 2008 in the Rize Province of northeastern Turkey. Pressed SBFS colonies with subtending apple cuticle were shipped to Iowa State University for isolation. Of 633 primary isolates from 148 apple peels, 87 cultures were purified, 67 isolates were genetically characterized and 33 isolates examined for morphology. The internal transcriber spacer (ITS) ribosomal DNA and a portion of the 28S large subunit region (LSU) were compared to previously identified fungi using parsimony analysis. Putative species were delineated from ITS sequences as well as morphology on apple and in culture. Twelve species were delineated based on parsimony analysis of ITS sequences and morphology. A newly discovered and described species from the survey was Scleroramularia abundans; newly discovered putative species included Zygophiala sp. FS3.3, Stomiopeltis spp. RS7.1 and RS7.2, and Chaetothyriales sp. F1; previously recovered putative species included Zygophiala sp. FS6 and Stomiopeltis sp. RS4.1; and previously discovered and named SBFS species included Schizothyrium pomi, Zygophiala wisconsinensis, Microcyclosporella mali, Microcyclospora tardicrescens, and Peltaster fructicola. Based on parsimony analysis of the LSU, one species was placed in the subclass Chaetothyriomycetidae and 11 species were placed in subclass Dothideomycetidae. Ten species were placed in the order Capnodiales. These findings expand the documented extent of genetic diversity within the worldwide SBFS complex and are the first published description of the composition of the SBFS complex from Turkey.


Black Sea Region Epiphytic fungi Malus Taxonomy SBFS 


  1. Batzer, J. C., Gleason, M. L., Harrington, T. C., & Tiffany, L. H. (2005). Expansion of the sooty blotch and flyspeck complex on apples based on analysis of ribosomal DNA gene sequences and morphology. Mycologia, 97, 1268–1286.PubMedCrossRefGoogle Scholar
  2. Batzer, J. C., Díaz-Arias, M. M., Harrington, T. C., Gleason, M. L., Groenewald, J. Z., & Crous, P. W. (2008). Four species of Zygophiala (Schizothyriaceae, Capnodiales) are associated with the sooty blotch and flyspeck complex on apple. Mycologia, 100, 246–258.PubMedCrossRefGoogle Scholar
  3. Batzer, J. C., Hernandez Rincon, S., Mueller, D. S., Petersen, B. J., Le Corronc, F., McManus, P. S., et al. (2010). Effect of temperature and nutrient concentration on the growth of six species of sooty blotch and flyspeck fungi. Phytopathologia Mediterranea, 49, 3–10.Google Scholar
  4. Batzer, J.C., Sisson, A.J., Harrington, T.C., Mayfield, D.A., Gleason, M.L. (2012). Temporal patterns in appearance of sooty blotch and flyspeck fungi on apples. Microbial Ecology, 64, 928–941.Google Scholar
  5. Blaser, J. M., Karakaya, A., Mayfield, D. A., Batzer, J. C., & Gleason, M. L. (2010). Diversity of sooty blotch and flyspeck from apples in northeastern Turkey. (Abstr.). Phytopathology, 100, S15.Google Scholar
  6. Colby, A. S. (1920). Sooty blotch of pomaceous fruits. Transactions of the Illinois Academy of Science, 13, 139–179.Google Scholar
  7. Díaz Arias, M. M., Batzer, J. C., Wang Wong, A., Bost, S. C., Cooley, D. R., Ellis, M. A., et al. (2010). Diversity and biogeography of sooty blotch and flyspeck fungi on apple in the eastern and midwestern United States. Phytopathology, 100, 345–355.PubMedCrossRefGoogle Scholar
  8. FAO Statistical Database. Retrieved February 10, 2012, from
  9. Fisher, N. L., Burgess, L. W., Tousson, T. A., & Nelson, P. E. (1982). Carnation leaves as a substrate and for preserving cultures of Fusarium species. Phytopathology, 72, 151–153.CrossRefGoogle Scholar
  10. Frank, J., Crous, P. W., Groenewald, J. Z., Oertel, B., Hyde, K. D., Phengsintham, P., et al. (2010). Microcyclospora and Microcyclosporella: novel genera accommodating epiphytic fungi causing sooty blotch on apple. Persoonia, 24, 93–105.PubMedCrossRefGoogle Scholar
  11. Gharghani, A., Zabihollah, Z., Talaie, A., Oraguzie, N. C., Fatahi, R., Hajnajari, H., et al. (2009). Genetic identity and relationships of Iranian apple (Malus x domestica Borkh.) cultivars and landraces, wild Malus species and representative old apple cultivars based on simple sequence repeat (SSR) marker analysis. Genetic Resources and Crop Evolution, 56, 829–842.CrossRefGoogle Scholar
  12. Gleason, M. L., Batzer, J. C., Guangyu, S., Zhang, R., Díaz-Arias, M. M., Sutton, T. B., et al. (2011). A new view of sooty blotch and flyspeck. Plant Disease, 95, 368–383.CrossRefGoogle Scholar
  13. Groves, A. B. (1933). A study of the sooty blotch disease of apples and casual fungus Gloeodes pomigena. Virginia Agriculture Experimental Station Bulletin, 50, 1–43.Google Scholar
  14. Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.Google Scholar
  15. Hickey, K.D. (1960). The sooty blotch and flyspeck diseases of apple with emphasis on variation within Gloeodes pomigena (SCW.) Colby. PhD dissertation, Pennsylvania State UniversityGoogle Scholar
  16. Ivanović, M. M., Ivanović, M. S., Batzer, J. C., Tatalović, N., Oertel, B., Latinović, J., et al. (2010). Fungi in the apple sooty blotch and flyspeck complex from Serbia and Montenegro. Journal of Plant Pathology, 92, 65–72.Google Scholar
  17. Li, H. Y., Zhang, R., Sun, G., Batzer, J. C., & Gleason, M. L. (2010). New species and record of Zygophiala on apple fruit from China. Mycological Progress, 9, 245–252.CrossRefGoogle Scholar
  18. Li, H. Y., Sun, G., Batzer, J. C., Crous, P. W., Groenewald, J. Z., Karakaya, A., et al. (2011). Scleroramularia gen. nov. associated with sooty blotch and flyspeck of apple and pawpaw form the Northern Hemisphere. Fungal Diversity, 46, 53–66.CrossRefGoogle Scholar
  19. Ridgway, R. (1912). Color standards and color nomenclature. Baltimore: Press of a Hoen and Company.Google Scholar
  20. Sutton, A. L., & Sutton, T. B. (1994). The distribution of the mycelial types of Gloeodes pomigena on apples in North Carolina and their relationship to environmental conditions. Plant Disease, 78, 668–673.CrossRefGoogle Scholar
  21. Swofford, D. L. (2002). PAUP. Phylogenetic Analysis Using Parsimony (and other methods). Version 4.0. Sunderland: Sinauer Associates.Google Scholar
  22. Tarnowski, T. B., Batzer, J. C., Gleason, M. L., Helland, S., & Dixon, P. (2003). Sensitivity of newly identified clades in the sooty blotch and flyspeck complex on apple to thiophanate-methyl and ziram. Online. Plant Health Progress. doi:10.1094/PHP-2003-12XX-01-RS.
  23. Vilgalys, R., & Hester, M. (1990). Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology, 172, 4238–4246.PubMedGoogle Scholar
  24. Voglmayr, H., Mayer, V., Maschwitz, U., Moog, J., Djieto-Lordon, C., & Blatrix, R. (2011). The diversity of ant-associated black yeasts: insights into a newly discovered world of symbiotic interactions. Fungal Biology, 115(10), 1077–1091.PubMedCrossRefGoogle Scholar
  25. White, T. J., Bruns, T., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Snisky, & J. W. White (Eds.), PCR protocols: A guide to molecular methods and amplifications (pp. 315–322). New York: Academic Press.Google Scholar
  26. Williamson, S. M., & Sutton, T. B. (2000). Sooty blotch and flyspeck of apple: etiology, biology, and control. Plant Disease, 84, 714–724.CrossRefGoogle Scholar
  27. Wrona, B., & Grabowski, M. (2004). Etiology of apple sooty blotch in Poland. Journal of Plant Protection Research, 44, 293–297.Google Scholar
  28. Yang, H. L., Sun, G. Y., Batzer, J. C., Crous, P. W., Groenewald, J. Z., & Gleason, M. L. (2010). Novel fungal genera and species associated with the sooty blotch and flyspeck complex on apple in China and the United States. Persoonia, 24, 29–37.PubMedCrossRefGoogle Scholar

Copyright information

© KNPV 2012

Authors and Affiliations

  • Derrick A. Mayfield
    • 1
  • Aziz Karakaya
    • 2
  • Jean C. Batzer
    • 1
  • Jennifer M. Blaser
    • 1
  • Mark L. Gleason
    • 1
  1. 1.Department of Plant Pathology and MicrobiologyIowa State UniversityAmesUSA
  2. 2.Department of Plant Protection, Faculty of AgricultureUniversity of AnkaraAnkaraTurkey

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