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European Journal of Plant Pathology

, Volume 132, Issue 1, pp 49–57 | Cite as

Effect of Meloidogyne ethiopica parasitism on water management and physiological stress in tomato

  • Polona StrajnarEmail author
  • Saša Širca
  • Gregor Urek
  • Helena Šircelj
  • Peter Železnik
  • Dominik Vodnik
Article

Abstract

Root-knot nematodes (RKN) are obligate endoparasites that severely damage the host root system. Nutrient and water uptake are substantially reduced in infested plants, resulting into altered physiological processes and reduced plant growth. The effect of nematode infestation on the morphological changes of roots and subsequent physiological plant responses of infested tomatoes with the RKN Meloidogyne ethiopica was studied in a pot experiment. Plants were infested with two inoculum densities (10 or 50 eggs per cm3 substrate) and its effect was evaluated 74 and 102 days post inoculation (DPI). Morphological changes and root growth was determined by analysing scanned images of the whole root system. Nematode infestation reduced the portion of fine roots and increased that of coarse roots due to gall formation. Fine roots of non-infested control plants represented around 51% of the area of the whole root system at 74 and 102 DPI. In comparison to controls, plants inoculated with low and high nematode density had 2.1 and 3.2-times lower surface area of fine roots at 102 DPI. Root analyses revealed that plants had a very limited ability to mitigate the effects of the root-knot nematodes infestation by altering root growth. Root galls had a major influence on the hydraulic conductivity of the root system, which was significantly reduced. The low leaf water potential of infested plants coincided with decreased stomatal conductivity, transpiration and photosynthesis. The latter two were reduced by 60–70% when compared to non-infested control plants.

Keywords

Leaf water potential Root hydraulic conductivity Root morphology Root-knot nematodes Photosynthesis 

Notes

Acknowledgements

This research was financially supported by the grant L4-1021 of the Slovenian Research Agency and the Ministry of Agriculture, Forestry and Food of the Republic of Slovenia. The authors thank to Dr. Hans-Josef Schroers for critically reviewing the manuscript.

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Copyright information

© KNPV 2011

Authors and Affiliations

  • Polona Strajnar
    • 1
    Email author
  • Saša Širca
    • 1
  • Gregor Urek
    • 1
  • Helena Šircelj
    • 2
  • Peter Železnik
    • 3
  • Dominik Vodnik
    • 2
  1. 1.Agricultural Institute of SloveniaLjubljanaSlovenia
  2. 2.Agronomy Department, Biotechnical FacultyUniversity of LjubljanaLjubljanaSlovenia
  3. 3.Slovenian Forestry InstituteLjubljanaSlovenia

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