European Journal of Plant Pathology

, Volume 130, Issue 3, pp 287–295 | Cite as

Effect of seed priming with Serratia plymuthica and Pseudomonas chlororaphis to control Leptosphaeria maculans in different oilseed rape cultivars

Original Research


The efficacy of a seed treatment of oilseed rape (OSR) (Brassica napus) with the rhizobacteria Serratia plymuthica (strain HRO-C48) and Pseudomonas chlororaphis (strain MA 342) applied alone or in combination against the blackleg disease caused by Leptosphaeria maculans was tested with different cultivars. Seeds were soaked in bacterial suspensions (bio-priming) to obtain log10 6–7 CFU seed−1. Cotyledons were inoculated with a 10 ul droplet of L. maculans spore suspension of log10 7 spores ml−1 and the disease index (size of lesions) was evaluated 14 days later. A mean disease reduction of 71.6% was recorded for S. plymuthica and of 54% for P. chlororaphis. The combined treatment was not superior to the treatment with S. plymuthica alone. The reduction of the disease caused by S. plymuthica was independent of the cultivar’s susceptibility, whereas the control effect recorded with P. chlororaphis increased with decreasing cultivar resistance to blackleg disease. The bacterial colonization of OSR was restricted to the roots and hypocotyl. No significant difference in bacterial colonization of the rhizosphere was observed between different cultivars, nor between single or combined bacterial seed treatments.


Brassica napus Blackleg Biological control Bio-priming Antagonists Root colonization 



The financial support of the German Academic Exchange Services (DAAD) is highly acknowledged and appreciated.


  1. Benhamou, N., Gagné, S., Le Quéré, D., & Dehbi, L. (2000). Bacterial-mediated induced resistance in cucumber: beneficial effect of the endophytic bacterium Serratia plymuthica on the protection against infection by Pythium ultimum. Phytopathology, 90, 45–56.PubMedCrossRefGoogle Scholar
  2. Bennett, A. J., & Whipps, J. M. (2008). Dual application of beneficial microorganisms to seed during drum priming. Applied Soil Ecology, 38, 83–89.CrossRefGoogle Scholar
  3. Berg, G., Fritze, A., Roskot, N., & Smalla, K. (2001). Evaluation of potential biocontrol rhizobacteria from different host plants of Verticillium dahliae Kleb. Journal of Applied Microbiology, 156, 75–82.Google Scholar
  4. Borges, A. A., Cools, H. J., & Lucas, J. A. (2003). Menadione sodium bisulphate: a novel plant defence activator which enhances local and systemic resistance to infection by Leptosphaeria maculans in oilseed rape. Plant Pathology, 52, 429–436.CrossRefGoogle Scholar
  5. Buchenauer, H. (1998). Biological control of soil-borne diseases by rhizobacteria. Journal of Plant Diseases and Protection, 105, 329–348.Google Scholar
  6. Compant, S., Duffy, B., Nowak, J., Clement, C., & Barka, E. A. (2005). Use of plant growth-promoting bacteria for biocontrol of plant diseases: principle, mechanisms of action, and future prospects. Applied and Environmental Microbiology, 71, 4951–4959.PubMedCrossRefGoogle Scholar
  7. De Vleeschauwer, D. & Höfte, M. (2007). Using Serratia plymuthica to control fungal pathogens of plants. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 2, No. 046.Google Scholar
  8. Fitt, B. D. L., Brun, H., Barbetti, M. J., & Rimmer, S. R. (2006). World-wide importance of phoma stem canker (Leptosphaeria maculans and L. biglobosa) on oilseed rape (Brassica napus). European Journal of Plant Pathology, 114, 3–15.CrossRefGoogle Scholar
  9. Frankowski, J., Lorito, M., Schmid, R., Berg, G., & Bahl, H. (2001). Purification and properties of two chitinolytic enzymes of Serratia plymuthica HRO-C48. Archieves of Microbiology, 176, 421–426.CrossRefGoogle Scholar
  10. Gaetan, S. A. (2005). First outbreak of blackleg caused by Phoma lingam in commertial canola fields in Argentina. Plant Disease, 89, 435.CrossRefGoogle Scholar
  11. Hammoudi, O. (2007). Einfluss mikrobieller Antagonisten auf den Befall mit Phoma lingam und Verticillium dahliae var. longisporum an Raps (Brassica napus L. var. napus). Dissertation, Christian-Albrechts-Universität zu Kiel.Google Scholar
  12. Hökeberg, M. (2006). Development and registration of biocontrol products—experience and perspectives gained from the bacterial seed treatment products Cedomon® and Cerall®. Proceedings of the International Workshop “Implementation of Biocontrol in Practice in Temperate Regions—Present and Near Future”. Research Centre Flakkebjerg, Denmark, November 1–3, 2005. DIAS Report, 119, p.77.Google Scholar
  13. Hökeberg, M., Gerhardson, B., & Johnsson, L. (1997). Biological control of cereal seed-borne diseases by seed bacterization with greenhouse-selected bacteria. Eurpean Journal of Plant Pathology, 103, 25–33.CrossRefGoogle Scholar
  14. Howlett, B. J. (2004). Current knowledge of the interaction beween Brassica napus and Leptosphaeria maculans. Canadian Journal of Plant Pathology, 26, 245–252.CrossRefGoogle Scholar
  15. Johnsson, L., Hökeberg, M., & Gerhardson, B. (1998). Performance of the Pseudomonas chlororaphis biocontrol agent MA 342 against cereal seed-borne diseases in field experiments. European Journal of Plant Pathology, 104, 701–711.CrossRefGoogle Scholar
  16. Khangura, R. K., & Barbetti, M. J. (2001). Prevalence of blackleg (Leptosphaeria maculans) on canola (Brassica napus) in Western Australia. Australian Journal of Experimental Agriculture, 41, 1–9.CrossRefGoogle Scholar
  17. Kurze, S., Dahl, R., Bahl, H., & Berg, G. (2001). Biological control of soil-borne pathogens in strawberry by Serratia plymuthica HRO-C48. Plant Diseases, 85, 529–534.CrossRefGoogle Scholar
  18. Müller, H., & Berg, G. (2008). Impact of formulation procedures on the effect of the biocontrol agent Serratia plymuthica HRO-C48 on Verticillium wilt in oilseed rape. BioControl, 53, 905–916.CrossRefGoogle Scholar
  19. Pang, Y., Liu, X., Ma, Y., Chernin, L., Berg, G., & Gao, K. (2009). Inducution of systemic resistance, root colonisiation and biocontrol activities of rhizospheric strain of Serratia plymuthica are dependent on N-acyl homoserine lactones. Eurpean Journal of Plant Pathology, 124, 261–268.CrossRefGoogle Scholar
  20. Spencer, M., Ryu, C. M., Yang, K. Y., Kim, Y. C., Kloepper, J. W., & Anderson, A. J. (2003). Induced defence in tobacco by Pseudomonas chlororaphis strain O6 involves at least the ethylene pathway. Physiological and Molecular Plant Pathology, 63, 27–34.CrossRefGoogle Scholar
  21. Tombolini, R., Goag, D. J., Gerhardson, B., & Jansson, J. K. (1999). Colonization pattern of the biocontrol strain Pseudomonas chlororaphis strain MA 342 on barely seeds visualized by using green fluorescent protein. Applied and Environmental Microbiology, 65, 3674–3680.PubMedGoogle Scholar
  22. West, J. S., Kharbanda, P. D., Barbetti, M. J., & Fitt, B. D. L. (2001). Epidemiology and management of Leptosphaeria maculans (phoma stem canker) on oilseed rape in Australia, Canada and Europe. Plant Pathology, 50, 10–27.CrossRefGoogle Scholar
  23. West, J. S., Fitt, B. D. L., Leech, P. K., Biddulph, J. E., Huang, Y. J., & Balesdent, M. H. (2002). Effects of timing of Leptosphaeria maculans ascospore release and fungicide regime on phoma leaf spot and phoma stem canker development on winter oilseed rape (Brassica napus) in southern England. Plant Pathology, 51, 454–463.CrossRefGoogle Scholar
  24. Whipps, J. (2001). Microbial interactions and biocontrol in the rhizosphere. Journal of Experimental Botany, 52, 487–511.PubMedGoogle Scholar

Copyright information

© KNPV 2011

Authors and Affiliations

  1. 1.Institute for Phytopathology, Department of Biotechnology and Biological ControlChristian-Albrechts-UniversityKielGermany

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