Morphological and molecular identification of Fusarium species associated with head blight on wheat in East Croatia
- 472 Downloads
Fusarium head blight (FHB) is an important fungal disease of wheat. The aim of this research was to determine the diversity of Fusarium species infecting winter wheat ears in East Croatia. In 2008 wheat kernels were obtained from three locations in the eastern part of Croatia (Tovarnik, Osijek, Pozega), and in 2009 from two additional locations (Slavonski Brod, Nova Gradiska). In total, 498 visually diseased kernels were selected for morphological identification of Fusarium spp. The identity of 226 selected isolates was further investigated by molecular techniques. The predominant species on wheat kernels in East Croatia in 2008 were F. graminearum, isolated and confirmed from more than 80% of sampled wheat kernels, followed by F. avenaceum (8%) and F. culmorum (7%). Incidence of F. poae was less than 2%. The most common species identified in 2009 were F. graminearum (50%), F. culmorum (13%), F. avenaceum (12%) and F. poae (7%). This is the first report on the identification of Fusarium species isolated from naturally infected wheat ears in Croatia.
KeywordsFusarium species Fusarium head blight Triticum aestivum East Croatia
Part of this study was supported by the Department for Agrobiotechnology, IFA-Tulln (Austria). The work presented in this paper is part of project ‘073-0730718-0598’, which is supported by MZOŠ RH.
- Abd-Elsalam, K. A., Ibrahim, N. A., Khalil, M. S., & Verreet, J. A. (2003). PCR identification of Fusarium genus based on nuclear ribosomal-DNA sequence data. African Journal of Biotechnology, 2(4), 82–85.Google Scholar
- Annual statistical yearbook (2007). Croatian central bureau of statistics in Republic of Croatia, from http://www.dzs.hr//default_e.htm.
- Demeke, T., Clear, R. M., Patrick, S. K., & Gaba, D. (2005). Species-specific PCR-based assays for the detection of Fusarium species and a comparison with the whole seed agar plate method and trichothecene analysis. International Journal of Food Microbiology, 103, 271–284.CrossRefPubMedGoogle Scholar
- Lemmens, M., Bűrstmayr, H., Krska, R., Schumacher, R., Grausgruber, H., & Ruckenbauer, P. (2004). The effect of inoculation treatment and long-term application of moisture on Fusarium head blight symptoms and deoxynivalenol contamination in wheat grains. European Journal of Plant Pathology, 110, 229–308.CrossRefGoogle Scholar
- Nelson, P. E., Toussoun, T. A., & Marasas, W. F. O. (1983). Fusarium species. An illustrated manual for identification. The Pennsylvania State University Press, University Park e Londra, PA, 193 ppGoogle Scholar
- Nirenberg, H. I. (1981). A simplified method for identifying Fusarium spp. occurring on wheat. Canadian Journal of Botany, 59, 1599–1609.Google Scholar
- Rahjoo, V., Zad, J., Javan-Nikkhah, M., Mirzadi Gohari, A., Okhovvat, S. M., Bihamta, M. R., et al. (2008). Morphological and molecular identification of Fusarium isolated from maize ears in Iran. Journal of Plant Pathology, 90(3), 463–468.Google Scholar