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European Journal of Plant Pathology

, Volume 128, Issue 4, pp 511–516 | Cite as

Morphological and molecular identification of Fusarium species associated with head blight on wheat in East Croatia

  • Valentina Spanic
  • Marc Lemmens
  • Georg Drezner
Article

Abstract

Fusarium head blight (FHB) is an important fungal disease of wheat. The aim of this research was to determine the diversity of Fusarium species infecting winter wheat ears in East Croatia. In 2008 wheat kernels were obtained from three locations in the eastern part of Croatia (Tovarnik, Osijek, Pozega), and in 2009 from two additional locations (Slavonski Brod, Nova Gradiska). In total, 498 visually diseased kernels were selected for morphological identification of Fusarium spp. The identity of 226 selected isolates was further investigated by molecular techniques. The predominant species on wheat kernels in East Croatia in 2008 were F. graminearum, isolated and confirmed from more than 80% of sampled wheat kernels, followed by F. avenaceum (8%) and F. culmorum (7%). Incidence of F. poae was less than 2%. The most common species identified in 2009 were F. graminearum (50%), F. culmorum (13%), F. avenaceum (12%) and F. poae (7%). This is the first report on the identification of Fusarium species isolated from naturally infected wheat ears in Croatia.

Keywords

Fusarium species Fusarium head blight Triticum aestivum East Croatia 

Notes

Acknowledgements

Part of this study was supported by the Department for Agrobiotechnology, IFA-Tulln (Austria). The work presented in this paper is part of project ‘073-0730718-0598’, which is supported by MZOŠ RH.

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Copyright information

© KNPV 2010

Authors and Affiliations

  1. 1.Department for Breeding & Genetics of Small Cereal CropsAgricultural Institute OsijekOsijekCroatia
  2. 2.IFA-TullnInstitute of Biotechnology in Plant ProductionTullnAustria

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