Mechanical transmission of Potato spindle tuber viroid between plants of Brugmansia suaveoles, Solanum jasminoides and potatoes and tomatoes
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Potato spindle tuber viroid (PSTVd) has been recently found in many solanaceous ornamental plant species. This study reports on the effectiveness of mechanical transmission between Brugmansia suaveolens, Solanum jasminoides, potato and tomato. Inoculation with ‘infected’ plant sap diluted in water, rubbing with contaminated finger tips and cutting with contaminated razor blades all resulted in transmission of PSTVd. Temperature, plant species and source of inoculum were found to be critical factors. An average temperature of 15°C only resulted in a few infections, whereas transmission at 20 and 25°C was more successful. Tomatoes were more susceptible to PSTVd than B. suaveolens, S. jasminoides and potatoes. Furthermore, S. jasminoides was a better source of inoculum than B. suaveolens. No transmission was obtained after repeated addition of inocula to tomato roots. These results indicate that PSTVd can be transmitted between plant species in practice by crop handling.
KeywordsPSTVd Pospiviroid Inoculation Inoculum Susceptibility Temperature
We thank our colleagues from the National Reference Laboratory of the Netherlands Plant Protection Service for their practical support. The work was partially performed in the frame of EU phytosanitary ERA-net EUPHRESCO project “Detection and Epidemiology of Pospiviroids” and financed by the Netherlands Ministry of Agriculture, Nature and Food Quality and the Slovenian Ministry of Agriculture, Forestry and Food.
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