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European Journal of Plant Pathology

, Volume 128, Issue 3, pp 399–407 | Cite as

Influence of rice development on the function of bacterial blight resistance genes

  • Kimberly M. Webb
  • Epifania Garcia
  • Casiana M. Vera Cruz
  • Jan E. Leach
Article

Abstract

Disease resistance genes most commonly used in breeding programs are single, dominant genes with relative effectiveness that is sometimes influenced by plant developmental stage. Knowing the developmental stages at which a resistance gene is functional is important for disease management. In rice, resistance at the seedling stage is crucial, because wounding during transplanting increases the potential for bacterial blight disease, and not all bacterial blight resistance genes are effective at the seedling stage. Effectiveness of the bacterial blight resistance genes Xa4, xa5, and Xa7, all in a common genetic background, was evaluated at different developmental stages by measuring lesion length and bacterial numbers after inoculation with the bacterial pathogen, Xanthomonas oryzae pv. oryzae. The Xa4 and xa5 genes controlled disease at all growth stages. In contrast, Xa7 was not fully functional in very young seedlings, but was completely effective by 21 days after sowing (das). The effects of plant developmental stage on interactions of the Xa7 gene with X. oryzae pv. oryzae strains carrying different mutant avrXa7 alleles were also tested. If a partial or fully functional avrXa7 allele was present, Xa7 resistance was effective at all growth stages tested after the transplant stage (>21 das).

Keywords

Aggressiveness Avirulence/effector gene Oryza sativa Virulence Xanthomonas oryzae pv. oryzae 

Abbreviations

Avr

avirulence gene

BB

bacterial blight

Dai

days after inoculation

Das

days after sowing

R gene

resistance gene

Xoo

Xanthomonas oryzae pv. oryzae

Notes

Acknowledgments

We would like to thank Tim Todd, Department of Plant Pathology, Kansas State University, who assisted in statistical data analysis. We also thank Florencio Balenson and Flavio Maghirang from the International Rice Research Institute (IRRI) in helping to maintain experiments and data collection. Travel for K. M. Webb to collect data was supported by the Asia Rice Foundation and the James B. Person Fellowship. This research was supported by the Generation Challenge Program, a USAID Linkage Project, and the Colorado State Agricultural Experiment Station.

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Copyright information

© KNPV 2010

Authors and Affiliations

  • Kimberly M. Webb
    • 1
    • 2
  • Epifania Garcia
    • 2
  • Casiana M. Vera Cruz
    • 2
  • Jan E. Leach
    • 3
  1. 1.USDA-ARS Sugarbeet Research UnitFt. CollinsUSA
  2. 2.Plant Breeding, Genetics, and BiotechnologyInternational Rice Research InstituteLos BañosPhilippines
  3. 3.Bioagricultural Sciences and Pest ManagementColorado State UniversityFt. CollinsUSA

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