Abstract
Ascochyta blight (AB, Ascochyta rabiei (Pass.) Lab.) is one of the most important foliar disease of chickpea (Cicer arietinum L.), globally. Chickpea is attacked by AB at any growth stage in cool and humid weather depending on the inoculum availability. However, the disease epidemics are most prominent during the flowering and podding growth stages. The main objective of this study was to determine the effect of growth stages of chickpea on the genetic resistance of AB and use this information in a resistance breeding program. Two susceptible and two moderately resistant chickpea cultivars were spray inoculated at seedling (GS1), post-seedling (GS2), vegetative (GS3), flowering (GS4) and podding (GS5) growth stages with A. rabiei conidial suspension under controlled environment conditions. Irrespective of crop cultivars the incubation period (IP) was shorter in GS1, GS4 and GS5 and was significantly extended in GS2 and GS3. Symptom development was delayed significantly in moderately resistant cultivars. The AB severity 10 days after inoculation ranged between 7 and 9 on susceptible cultivars and 3 and 5 on moderately resistant cultivars. Further the correlation coefficient of disease severity between GS1, GS4 and GS5 was highly significant (r = 0.95) indicating that, evaluation for resistance to AB can be done at GS 1 (seedling stage), and or GS4 (flowering stage) to GS5 (podding stage) growth stages of chickpea. This supports the evaluation for AB resistance using 10-day-old-seedlings in controlled environment at ICRISAT and adult plant field screening at hot-spot locations in Dhaulakuan and Ludhiana in India.
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References
Basandrai, A. K., Pande, S., Kishore, G. K., Crouch, J. H., & Basandrai, D. (2005). Cultural, morphological and pathological variation in Indian isolates of Ascochyta rabiei, the chickpea blight pathogen. Plant Pathology Journal, 21, 207–213.
Basandrai, A. K., Basandrai, D., Pande, S., Sharma, M., Thakur, S. K., & Thakur, H. L. (2007). Development of Ascochyta blight (Ascochyta rabiei) in chickpea as affected by host resistance and plant age. European Journal of Plant Pathology, 11, 77–86.
Chongo, G., & Gossen, B. D. (2001). Effect of plant age on resistance to Ascochyta rabiei in chickpea. Canadian Journal of Plant Pathology, 23, 358–363.
Chongo, G., Gossen, B. D., & Buchawldt, L. (2000). Fungicidal control of Ascochyta blight in chickpea in 1999. In 1999 Pesticide Management Research Reports (pp. 275–276). Agriculture and Agri Foods Canada, Ottawa Ontario. Report No. 99.
FAO. 2008. FAO Bulletin of statistics. http://faostat.org/faostatcollectionsubset=agriculture.
Gan, Y. T., Siddique, K. H. M., MacLeod, W. J., & Jayakumar, P. (2006). Management options for minimizing the damage by Ascochyta blight (Ascochyta rabiei) in chickpea (Cicer arietinum L.). Canadian. Journal of Plant Sciences, 82, 531–537.
Gaur, R. B., & Singh, R. D. (1996). Evaluation of chickpea cultivars for resistance to Ascochyta blight. Indian Journal of Mycology and Plant Pathology, 26, 50–55.
Hanselle, T., & Barz, W. (2001). Purification and characterization of the extracellular PR-2b beta-1, 3-glucanase accumulating in different Ascochyta rabiei-infected chickpea (Cicer arietinum L.) cultivars. Plant Science, 161, 773–781.
Kaiser, W. J., Coca, F. W., & Vega, S. (2000). First report of Ascochyta blight of chickpea in Latin America. Plant Disease, 84, 102.
Knights, E. J., & Siddique, K. H. M. (2002). Chickpea status and production constraints in Australia. In M.A. Bakr, K.H.M. Siddique, & C. Johansen (Eds.), Integrated management of Botrytis grey mould of chickpea in Bangladesh and Australia. Summary proceedings of a project inception workshop, 1–2 June 2002. (pp. 33–41). (Bangladesh Agricultural Research Institute: Joydebpur, Bangladesh).
Littell, R. C., George, A. M., Walter, W. S., Russell, D. W., & Oliver, S. (2006). SAS® for Mixed Models (2nd ed.). Cary: SAS Institute Inc.
Nehra, K. S., Sindhu, A., Singh, R., Batra, P., Dhillon, S., & Sindhu, A. (1997). Induction and characterization of exochitinase from chickpea (Cicer arietinum L.) infected with Ascochyta rabiei. Annals of Biology, 13, 305–308.
Nene, Y. L., & Reddy, M. V. (1987). Chickpea diseases and their control. In M. C. Saxena & K. B. Singh (Eds.), The Chickpea (pp. 233–270). Wallingford: CAB International.
Nene, Y. L., Haware, M. P. & Reddy, M. V. (1981). Chickpea diseases: resistance-screening techniques. Information Bulletin No. 10. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Andhra Pradesh, India, 10 pp.
Pande, S., Siddique, K. H. M., Kishore, G. K., Bayaa, B., Gaur, P. M., Gowda, C. L. L., et al. (2005). Ascochyta blight of chickpea: biology, pathogenicity, and disease management. Australasian Journal of Agricultural Research, 56, 317–332.
Pande, S., Sharma, M., Kaur, L., Basandrai, A. K., Gaur, P. M., Khan, T., Siddique, K. H. M., & Gowda, C. L. L. (2009). Development of screening techniques and identification of new sources of resistance to Ascochyta blight disease of chickpea. (Paper presented in Proceedings of Ascochyta, the second International Workshop, June 28–July 2, 2009, Pullman, Washington, USA). 63 p.
Pedersen, E. A., & Morrall, R. A. A. (1994). Effect of cultivar, leaf wetness duration, temperature, and growth stage on infection and development of Ascochyta blight of lentil. Phytopathology, 84, 1024–1030.
Reddy, M. V., & Singh, K. B. (1984). Evaluation of a world collection of chickpea germplasm accessions for resistance to Ascochyta blight. Plant Disease, 68, 900–901.
Sharma, M., Pathak, M., Rao, J. N., & Pande, S. (2006, January-February). Comparison of resistance screening techniques for Ascochyta blight and Botrytis gray mold in chickpea. (Paper presented in 58th Annual Meeting of Indian Phytopathological Society and National Symposium on Emerging Plant Diseases their Diagnosis and Management. University of North Bengal, Siliguri, West Bengal, India).
Singh, G., & Sharma, Y. R. (1998). Ascochyta blight of chickpea. In R. K. Upadhayay, K. G. Mukherji, & R. L. Rajak (Eds.), IPM system in agriculture: Pulses (pp. 163–195). New Delhi: Aditya Books Pvt. Ltd.
Trapero-Casas, A., & Kaiser, W. J. (1992). Influence of temperature, wetness period, plant age and inoculum concentration on infection and development of Ascochyta blight. Phytopathology, 82, 589–596.
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Sharma, M., Pande, S. & Rathore, A. Effect of growth stages of chickpea on the genetic resistance of Ascochyta blight. Eur J Plant Pathol 128, 325–331 (2010). https://doi.org/10.1007/s10658-010-9641-x
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DOI: https://doi.org/10.1007/s10658-010-9641-x