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European Journal of Plant Pathology

, Volume 128, Issue 1, pp 65–70 | Cite as

Genetic resistance to bacterial blight disease in Persian walnut

  • Jalal Soltani
  • Ali A. Aliabadi
Article

Abstract

Bacterial blight disease of Persian walnut (Juglans regia, L.), caused by Xanthomonas arboricola pv. juglandis (Xaj), leads to significant nut losses in northern, central and western areas of Iran. To identify the natural sources of resistance to disease in the endemic walnut genotypes of Iran, sixteen walnut genotypes, collected from different areas of Hamedan province, were inoculated with Xaj in a randomized complete block design with five replicates for each genotype. Two-year old genotypes were gently sprayed with a suspension of bacteria adjusted to approximately 2 × 109 cfu ml−1 of distilled water in May. Infected leaves were rated for disease 28 and 42 days after inoculation, using a 0 to 5 severity scale, based on the number, size and distribution of lesions on the leaves. Data analyses showed that there were variations among genotypes in response to pathogen. Upon inoculation by bacterial suspension genotype 94 showed the highest resistance to both disease incidence and its progress after 4–6 weeks of infection. Genotype 65 showed high susceptibility to disease and genotype 69 showed high susceptibilities both to disease incidence and its progress after 4–6 weeks of infection.

Keywords

Juglans regia Xanthomonas arboricola pv. juglandis Genetic variation Disease resistance 

Abbreviations

Xaj

Xanthomonas arboricola pv. Juglandis

Cfu

Colony forming units

Notes

Acknowledgements

We would like to thank Farid Beiki and Hossein Hokmabadi for their technical support and discussions. The researchers of Agricultural Office of Hamedan: Zarei, Faramarzi & Asadian, are appreciated for providing the walnut genotypes. Forests and Pastures Organization of Karaj, Tehran, is appreciated for providing the lab, the experimental field, and research facilities. This research was financed by MSRT of Iran.

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Copyright information

© KNPV 2010

Authors and Affiliations

  1. 1.Phytobacteriology section, Phytopathology DepartmentBu-Ali Sina University (BASU)HamedanIran
  2. 2.Alborz Research CenterForests and Pastures OrganizationKarajIran

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