European Journal of Plant Pathology

, Volume 127, Issue 1, pp 137–148 | Cite as

The effect of different carbon sources on phenotypic expression by Fusarium graminearum strains

Original Research


Two Fusarium graminearum strains were cultured in glucose yeast extract peptone broth or minimal medium broth to measure the production of mycelial biomass, pH, mycotoxins, and aurofusarin pigment, when limited to single carbon sources (at 1%), including xylan, cellulose, starch, or glucose. A random complete block design with factorial arrangement and analysis of variance at a significance level of 0.01 were employed to test for treatment differences. Overall, the F. graminearum strains produced significantly more biomass, deoxynivalenol, and aurofusarin with xylan than with cellulose. No significant differences were found in terms of 15–acetyldeoxynivalenol production from the four carbon sources. The presence of significant interactions between the strains, carbon sources, and media led to the following specific differences. In yeast extract peptone broth, R-9828 strain yielded significantly more deoxynivalenol production with xylan than cellulose and R-9832 produced significantly more mycelium (biomass) with xylan than cellulose. R-9828 strain yielded significantly more deoxynivalenol production than the R-9832 strain. Also in yeast extract peptone broth, cellulose led to significantly higher pH values than other carbons, which might be due to the limited ability of the Fusarium strains to utilize cellulose as an energy source. Aurofusarin was the only expressed analyte to show a significant difference in minimal medium broth, and R-9832 produced significantly more aurofusarin with xylan than with cellulose in the broth. These results suggest that xylan may induce Fusarium growth and deoxynivalenol production to assist the infection process and may support the theory that F. graminearum invades through xylan in the cell walls of cereals.


Trichothecene Aurofusarin Xylan Cellulose pH Biomass 



Analysis of Variance


Absorbance Unit








carnation leaf agar




Fusarium head blight


glucose yeast extract peptone broth


high performance liquid chromatography


Minimal medium broth




photodiode array detector


random complete block design




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Copyright information

© KNPV 2010

Authors and Affiliations

  1. 1.Department of Dairy SciencesSouth Dakota State UniversityBrookingsUSA
  2. 2.Department of Veterinary and Microbiological SciencesNorth Dakota State UniversityFargoUSA

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