Transmission of Tomato chlorotic dwarf viroid by bumblebees (Bombus ignitus) in tomato plants
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Quantitative PCR revealed that Tomato chlorotic dwarf viroid (TCDVd) was present in substantial amounts in viroid-infected tomato flowers. Healthy tomato plants were arranged in two different glasshouses, and plants were mechanically inoculated with TCDVd. Bumblebees (Bombus ignitus) were then introduced into the glasshouses to reveal whether the viroid was transmitted from infected source plants to neighbouring healthy plants. TCDVd infection was found in neighbouring tomato plants more than 1 month after the introduction of the bees, some of which expressed symptoms, in both glasshouses. Thus, bumblebees transmitted TCDVd from tomato to tomato by pollination activities.
KeywordsAnther Pollination Potato spindle tuber viroid Quantitative PCR Rutgers
We are grateful to Dr. Taeko Takeuchi of Chiba Prefectural Agriculture and Forestry Research Centre, Japan for her advice. This work was supported in part by Research Project (No. 1976) for the Utilisation of Advanced Technologies in Agriculture, Forestry and Fisheries, of the Ministry of Agriculture, Forestry and Fisheries of Japan.
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